Navigation Links
New Insight Into the Controls on a Go-To Enzyme
Date:11/19/2008

he calpastatin molecule attach to calpain in the inhibition process," said Green, the report's senior author. "However, there was no overall picture of calpastatin that revealed how it was so precise in its attachment and potent in its function."

To obtain that overall picture, St. Jude researchers used the analytical technique of X-ray crystallography, with help from nuclear magnetic resonance (NMR) spectroscopy. In this widely used method of determining protein structure, researchers first crystallize a protein to be studied. Then, they direct X-rays through the crystal and deduce the protein structure from the diffraction pattern of those X-rays. To overcome the crystallization bottleneck, a lengthy and unpredictable variable in X-ray crystallography, the investigators used NMR spectroscopy to tailor the perfect enzyme-inhibitor complex.

Tudor Moldoveanu, Ph.D., a postdoctoral fellow in Green's laboratory, performed X-ray structural analysis on such a protein crystal that consisted of a critical part of the calpastatin molecule attached to calpain. The structural picture obtained of the two proteins clutched together clearly revealed why calpastatin so specifically attaches to calpain.

"Calpain has multiple domains, and what we saw was that calpastatin wraps itself around pretty much every domain of calpain," said Moldoveanu, the report's first author. This attachment not only blocks the portion of the enzyme called the active site, where calpain performs its snipping function, but also covers regions away from that site. Such a broad molecular embrace guarantees that calpastatin will potently and rapidly shut down calpain's function, Moldoveanu said. This broad embrace also guarantees that calpastatin will precisely recognize only calpains, rather than mistakenly attach to other similar enzymes in the cell.

Furthermore, the researchers discovered how calpastatin evades being che
'/>"/>

SOURCE St. Jude Children's Research Hospital
Copyright©2008 PR Newswire.
All rights reserved

Page: 1 2 3

Related medicine technology :

1. St. Jude Study Gives New Insights Into How Cells Accessorize Their Proteins
2. Nemours Center for Childhood Cancer Research Team at the Alfred I. duPont Hospital for Children Identifies the Function of a Biomarker Present in Prostate Cancer Cells: New Insight into Therapeutic Benefit for Patients with Advanced Prostate Cancer
3. New Data Offers Insights Into the Potential Role for TYKERB(R) (Lapatinib) in Non-Cytotoxic Chemotherapy-Based Treatment Regimens
4. Apieron, Inc. Begins Customer Shipments of the Insight(TM) eNO System
5. Genmab A/S - Company Announcement: Novel Insights into HuMax-EGFr Mechanisms of Action Published in PNAS
6. Not Only Lipids and Inflammation: Insight Into a New Cause of Heart Attack and Other Vascular Disease
7. PDHI Launches ConXus Rewards, Announces Partnership With Hallmark Insights to Support Incentive-Driven Wellness Programs
8. Chemstar Corporation Announces a New Powerful, One-Step Product That Effectively Controls Listeria, Salmonella, E. coli and Fly Larvae in Floor Drains
9. RAD001 Combined with Sandostatin LAR(R) Depot and as Monotherapy Controls Growth of Rare Pancreatic Neuroendocrine Tumors
10. Enobia Pharma Initiates Clinical Testing of Enzyme Replacement Therapy to Treat Hypophosphatasia
11. Genzyme and Isis Begin Second Phase 3 Trial Of Mipomersen
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2015)... , March 31, 2015 ... "Life Science and Chemical Instrumentation Market (Chromatography, Electrophoresis, DNA ... Flow Cytometer, Incubator, Fume Hood, Centrifuge) - Global ... Life Science and Chemical Instrumentation Market is expected ... of 6.9% from 2014 to 2019. ...
(Date:3/31/2015)... , March 31, 2015  Mark Farrah Associates ... Medicare Advantage (MA) plans in their recent analysis ... people were enrolled in Medicare Advantage plans as ... 1.3 million members year-over-year.  54.3 million people are ... Medicare and Medicaid Services (CMS), with an unpenetrated ...
(Date:3/31/2015)... , March 31, 2015  On May ... will convene in Santa Monica, CA ... on Lung-Sparing Therapies for Malignant Pleural Mesothelioma (MPM), ... exposure to asbestos and commonly affects the lining ... co-hosted by UCLA and the Pacific Mesothelioma Center ...
Breaking Medicine Technology:Life Science and Chemical Instrumentation Market Worth $48.84 Billion by 2019 2Life Science and Chemical Instrumentation Market Worth $48.84 Billion by 2019 3Life Science and Chemical Instrumentation Market Worth $48.84 Billion by 2019 4Life Science and Chemical Instrumentation Market Worth $48.84 Billion by 2019 5Medicare Advantage Enrollment Reaches 17 Million According to Mark Farrah Associates 2Cutting-Edge Research to Be Presented at 5th Annual International Symposium on Lung-Sparing Therapies for Malignant Pleural Mesothelioma in Santa Monica 2Cutting-Edge Research to Be Presented at 5th Annual International Symposium on Lung-Sparing Therapies for Malignant Pleural Mesothelioma in Santa Monica 3Cutting-Edge Research to Be Presented at 5th Annual International Symposium on Lung-Sparing Therapies for Malignant Pleural Mesothelioma in Santa Monica 4
(Date:3/31/2015)... 31, 2015 Students, teachers, and the ... education in a drastically different way as current and ... and training content is being delivered and received. , ... technology in an unprecedented way with education becoming individualized, ... co-founder and CEO, My Mobile University , This ...
(Date:3/31/2015)... Dr. St. Ledger recently returned from yet another trip ... Here, he participated in the Worn Dentition Workshop: a ... in cases of severely worn teeth, as well as the ... correlation to GERD and Sleep Apnea. , Dr. ... After the workshop he attended, he mentored for three days ...
(Date:3/31/2015)... 2015 Researchers at UC Davis ... humans: how human livers heal. The difference centers ... regeneration. Normally, mouse PPARa is far more active ... to quickly regenerate damaged livers. However, the research ... can boost the regenerative effects of human PPARa. ...
(Date:3/31/2015)... Princeton, NJ (PRWEB) March 31, 2015 ... a white paper entitled, “The Value of an ... report continues to play a critical role in ... health systems. With the appropriate understanding and review, ... decision support and strategic planning. , This white ...
(Date:3/31/2015)... With intravenous (IV) infusion therapy, medication ... Patients, typically, are prescribed IV infusion therapy when their ... no longer effective. Illness often leads to dehydration, ... electrolyte imbalances. Infusion therapy benefits the patient’s recovery ... treating dehydration, IV infusion therapy can be used to ...
Breaking Medicine News(10 mins):Health News:LTT Global Communications Launches First Asia Holistic Online Learning Platform 2Health News:LTT Global Communications Launches First Asia Holistic Online Learning Platform 3Health News:The dentists at Upper Montclair Dental Associates are happy to announce exciting new happenings at their practice. 2Health News:UC Davis Study Finds Protein May Improve Liver Regeneration 2Health News:UC Davis Study Finds Protein May Improve Liver Regeneration 3Health News:UC Davis Study Finds Protein May Improve Liver Regeneration 4Health News:Intravenous Infusion Therapy Now Available at Urgent Care of Texas Centers; Hospital-Level Care in an Outpatient Setting. 2
... University from the National Institutes of Health,s Fogarty International ... in Health Informatics. The new center will ... informatics programs at IU and the Regenstrief Institute with ... Africa at Moi University and Moi Teaching and Referral ...
... drug by adolescents, with almost 42% of high school ... use may result in a number of serious consequences ... of cancer. As such, it is critical to prevent ... scientists have been trying to establish the best means ...
... , ... Medicare, and Medicaid Plans Recognized Among The Nation,s Top 25, , ... Albany, N.Y. (Vocus) November 16, 2009 -- Underscoring ... was ranked as the top-rated health plan in New York state, according to the ...
... , Accepts Report of Blue Ribbon Advisory Panel ... 21st century care, Secretary of Veterans Affairs Eric K. Shinseki ... Veterans Affairs (VA) and the schools that train the nation,s ... of higher education for health care professionals have been essential ...
... African Services Committee Applauds City for Its Efforts to ... YORK, Nov. 16 African Services Committee is praising New ... putting the second-generation female condom into the hands of women ... enthusiastically endorse New York City,s efforts to scale up access ...
... , , FRANKLIN, Tenn., Nov. 16 ... point of agreement by all parties is that some form ... that now is the worst time for government-mandated change because ... industry. One Franklin Tennessee-based software company is defining that innovation ...
Cached Medicine News:Health News:Indiana University receives NIH grant to improve health care in East Africa 2Health News:Indiana University receives NIH grant to improve health care in East Africa 3Health News:The straight dope: Studies link parental monitoring with decreased teen marijuana usage 2Health News:CDPHP Rated Top Plan In New York State By U.S. News & World Report/NCQA America's Best Health Insurance Plans 2009-10* 2Health News:CDPHP Rated Top Plan In New York State By U.S. News & World Report/NCQA America's Best Health Insurance Plans 2009-10* 3Health News:Secretary Enhancing Partnerships with Medical Schools 2Health News:NYC's African Diaspora Community Welcomes the Second-Generation Female Condom (FC2) 2Health News:NYC's African Diaspora Community Welcomes the Second-Generation Female Condom (FC2) 3Health News:Health Care Robots: Next Generation Decision-Making Software, Eliminates Manual Systems Labor 2Health News:Health Care Robots: Next Generation Decision-Making Software, Eliminates Manual Systems Labor 3
Portless Sheath Introducers...
Introducer Sheath Designed for Vascular Access...
Used to introduce balloon, electrode, closed or non-tapered end and,other catheters....
... Driver Stent design, utilizing an ... limitations of stainless steel stents. ... thinner struts, for unparalleled flexibility ... Driver Coronary Stent Systemwith thin ...
Medicine Products: