Navigation Links
Researchers find 2 new methods to determine ALK status
Date:7/1/2013

DENVER The implementation of personalized health care in cancer relies on the identification and characterization of cancer biomarkers and the availability of accurate detection systems and therapies for those biomarkers. Anaplastic lymphoma kinase (ALK), a tyrosine kinase, is a more recently characterized cancer biomarker in nonsmall-cell lung cancer (NSCLC). To identify NSCLC patients with ALK gene rearrangement in clinical trials, researchers have used the methods known as fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC). While IHC is a less complex and less costly technology than FISH, both methods present challenges.

Now research published in the August issue of the Journal of Thoracic Oncology (JTO), describes the development and evaluation of two new methodologies.

To improve IHC assay sensitivity, the researchers incorporated the novel, nonendogenous hapten 3-hydroxy-2-quinoxaline and tyramide amplification into a diaminobenzidine and horseradish peroxidasebased assay. The new detection system proved to be very useful for detecting low levels of ALK protein expression in NSCLC.

They also developed a brightfield IHCin situ hybridization combination assay (geneprotein assay) for the concurrent visualization of ALK protein and ALK gene arrangement. This allows the concurrent visualization of ALK gene and ALK protein status in single cells, allowing more accurate ALK status determination even in heterogeneous specimens.

The authors say, "this tool for simultaneously assessing both ALK protein expression (IHC) and ALK gene rearrangement (ISH) in NSCLC will be valuable for research on the mechanisms driving ALK-dependent malignancies and as a model of new diagnostic approach for identifying patients who might benefit from ALK-targeted therapies. More generally, it also provides proof of concept for the development of new methodologies for the simultaneous assessment of gene structure and protein-expression status in a single cell.


'/>"/>

Contact: Kristal Griffith
Kristal.Griffith@iaslc.org
720-325-2952
International Association for the Study of Lung Cancer
Source:Eurekalert

Related medicine news :

1. CWRU researchers trace inner-city womens health issues to childhood traumas
2. Penn Medicine researchers discover link between fear and sound perception
3. UCI researchers awarded $2.27 million to create novel diabetes treatments
4. MS researchers determine that brain reserve independently protects against cognitive decline in MS
5. Notre Dame and Harper researchers developing novel method to test for HPV and oral cancers
6. PhD Thesis Writing Expands the Scope of its Services for Doctoral Researchers
7. Researchers Identify New Genes Linked to the Origins of Alzheimer’s, Says Cure Alzheimers Fund
8. Researchers identify master coordinator for aortic rupture
9. Fate of the heart: Researchers track cellular events leading to cardiac regeneration
10. Moffitt Cancer Center researchers identify genetic variants predicting aggressive prostate cancers
11. Timely treatment after stroke is crucial, UCLA researchers report
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... ... June 27, 2016 , ... Quality metrics are proliferating in cancer care, ... remain in the eye of the beholder, according to experts who offered insights and ... Journal of Managed Care. For the full issue, click here . , For ...
(Date:6/26/2016)... ... June 26, 2016 , ... ... they have been diagnosed with endometriosis. These women need a treatment plan to ... a comprehensive approach that can help for preservation of fertility and ultimately achieving ...
(Date:6/25/2016)... ... June 25, 2016 , ... First Choice Emergency Room , the ... Ogunleye, as the Medical Director of its new Mesquite-Samuell Farm facility. , “We ... new Mesquite location,” said Dr. James M. Muzzarelli, Executive Medical Director of First Choice ...
(Date:6/24/2016)... CA (PRWEB) , ... June 24, 2016 , ... A ... procedures that most people are unfamiliar with. The article goes on to state that ... but also many of these less common operations such as calf and cheek reduction. ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Global law ... magazine’s 2016 Legal Elite. The attorneys chosen by their peers for this recognition are ... , Seven Greenberg Traurig Shareholders received special honors as members of this year’s Legal ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... Research and Markets has announced the addition of the ... to 2022" report to their offering. ... financial data derived from varied research sources to present unique ... on the market during the next five years, including a ... markets, regional and country level analysis. The report provides a ...
(Date:6/23/2016)... 23, 2016 Research and Markets has ... 52" report to their offering. ... creates a favourable commercial environment for MedImmune to enter. The ... that will serve to drive considerable growth for effective anti-influenza ... to cap sales considerably, but development is still in its ...
(Date:6/23/2016)... June 23, 2016 , , ... July 7, 2016 , , , , LOCATION: , , ... , , , EXPERT PANELISTS:  , , , Frost & ... Analyst, Christi Bird; Senior Industry Analyst, Divyaa Ravishankar and Unmesh Lal, ... The global pharmaceutical industry is witnessing an exceptional era. Several new ...
Breaking Medicine Technology: