Navigation Links
Capturing circulating cancer cells could provide insights into how disease spreads
Date:12/11/2012

ANN ARBORA glass plate with a nanoscale roughness could be a simple way for scientists to capture and study the circulating tumor cells that carry cancer around the body through the bloodstream.

Engineering and medical researchers at the University of Michigan have devised such a set-up, which they say takes advantage of cancer cells' stronger drive to settle and bind compared with normal blood cells.

Circulating tumor cells are believed to contribute to cancer metastasis, the grim process of the disease spreading from its original site to distant tissues. Blood tests that count these cells can help doctors predict how long a patient with widespread cancer will live.

As important as the castaway cells are, scientists don't know a lot about them. They're rare, at about one per billion blood cells. And they are not all identical, even if they come from the same tumor. Existing tools for isolating them only catch certain types of cellsthose that express specific surface proteins or are larger than normal blood cells.

For example, the commonly used, FDA-approved CellSearch system uses antibody- coated magnetic beads to seek out tumor cells and bind to them. But not all circulating tumor cells express the proteins these antibodies recognize. It is possible that the most dangerous ones, known as cancer stem or progenitor cells, may have shed that tell-tale coat, thereby evading approaches that rely on antibodies.

The researchers say their system could likely trap these stealth cancer stem cellsa feat no research team has accomplished yet.

"Our system can capture the majority of circulating tumor cells regardless of their surface proteins or their physical sizes, and this could include cancer progenitor or initiating cells," said Jianping Fu, assistant professor of mechanical engineering and biomedical engineering and a senior author of a paper on the technique published online in ACS Nano.

Fu and his engineering colleagues teamed up with U-M senior cancer researcher and breast cancer clinician Dr. Sofia Merajver and her team. This multidisciplinary group believes that while the device could one day improve cancer diagnosis and prognosis, its first uses would be for researchers to isolate live circulating tumor cells from blood specimens and study their biological and physical properties.

"Understanding the physical behavior and nature of these circulating tumor cells will certainly help us understand better one of the most difficult questions in cancer biologythe metastatic cascade, that is, how the disease spreads," Fu said. "Our system could provide an efficient and powerful way to capture the live circulating tumor cells and use them as a surrogate to study the metastatic process."

But capturing them, as challenging as it has proven to be, is only the beginning, said Merajver, who has spent the last 18 years studying cell signaling and the physical properties of highly aggressive cancer cells.

"The application of integrative biology is necessary to put together the story of how these cells behave in time to achieve successful metastases and thereby discover the routes to suppressing this deadly development," Merajver said. "Our collaboration with the Fu lab exemplifies the innovation needed for the war against cancerteam science from the lab all the way to the clinic."

In their experiments, the researchers used a standard and inexpensive microfabrication technique called "reactive ion etching" to roughen glass slides with a nanoscale resolution. Then, they spiked different blood samples with cancer cells derived from human breast, cervical and prostate tissues. When they poured the samples over the glass plates, the nanorough glass surfaces captured an average of 88 percent to 95 percent of the cancer cells.

Fu suggests why.

"Blood cells are intrinsically floating," Fu said. "Cancer cells including circulating tumor cells derived from solid tumors are presumably adherent cells. They can escape from the primary tumor while maintaining certain adhesion properties that allow them to attach and establish another tumor."

In other studies, researchers have noticed that circulating tumor cells tend to stick to rough surfaces. But the rough surfaces in those studies were coated with capture antibodies. These new nanorough surfaces do not require capture antibodies.

"Our method presents a significant improvement as it can be applied in principle to any cancer cell that comes from solid tumors," Fu said.

The paper is titled "Nanoroughened Surfaces for Efficient Capture of Circulating Tumor Cells without Using Capture Antibodies." The university is pursuing patent protection for the intellectual property and is seeking commercialization partners to help bring the technology to market.


'/>"/>

Contact: Nicole Casal Moore
ncmoore@umich.edu
734-647-7087
University of Michigan
Source:Eurekalert

Related medicine news :

1. Coffee May Lower Risk of Death From Mouth Cancer: Study
2. The current state of lung cancer treatment
3. Children born prematurely are at higher risk of esophageal inflammation, cancer
4. FDA OKs Expanded Use of Prostate Cancer Drug
5. Prostate cancer now detectable by imaging-guided biopsy
6. Targeted prostate biopsy has potential to improve diagnosis of prostate cancer
7. UCLA cancer scientists identify liposarcoma tumors that respond to chemotherapy
8. The Project for Natural Health Choices Inc. Issues an Advisory for Men to Consume Flavonoids in their Diet to Protect Against Prostate Cancer
9. To fight incurable metastatic breast cancer, resistance must be broken
10. Caffeinated coffee may reduce the risk of oral cancers
11. Abnormal DNA maintenance related to cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/26/2017)... ... September 26, 2017 , ... Atmosera , a premier ... as a Service (RMaaS) to automate the deployment of websites and applications ... e-tailers, and web development agencies who want to improve time-to-revenue with a reliable, ...
(Date:9/26/2017)... Barcelona, Spain (PRWEB) , ... September 26, 2017 ... ... exciting nutritional ingredient that is not only entirely new and different, it is ... extracts, announced today that the company has received an exclusive license agreement with ...
(Date:9/26/2017)... ... September 26, 2017 , ... After ... R. Elliott HERO Campaign for Designated Drivers proudly announced the winners of the ... local bars and restaurants took on the challenge of competing in three categories: ...
(Date:9/26/2017)... ... September 26, 2017 , ... Data Integrity: , The Key ... Dec. 5-6, 2017 – Arlington, VA, http://www.fdanews.com/fdadataintegrity , Consider ... the firm’s electronic records may not be trustworthy and reliable … a more detailed ...
(Date:9/26/2017)... ... September 26, 2017 , ... Dr. Kovatis, ... of the Leg, Ankle, and Foot Section of the Department of Orthopedic Surgery ... orthopedic surgeon at Hackensack UMC performing total ankle replacements. Because of this, Dr. ...
Breaking Medicine News(10 mins):
(Date:9/13/2017)... 2017   OrthoAtlanta has been named the official ... Committee (AFHC) for the 2018 College Football Playoff (CFP) National ... Mercedes-Benz Stadium in Atlanta, Georgia . OrthoAtlanta ... In" campaign, participating in many activities leading up to, and ... ...
(Date:9/12/2017)... MOUNTAIN LAKES, N.J. , Sept. 12, 2017  Consumer reviews ... Embrace Hearing as the number one company for hearing ... Oticon™, ReSound™ and fifteen other brands. ... Embrace Hearing Named #1 by Consumers For Hearing Aids ... Embrace Hearing is an online store that ...
(Date:9/9/2017)... ... ... – Monday, September 18 th .The Brain Tumor Foundation (BTF) begins ... the public.Where:  BTF,s Mobile MRI Unit – a ... Washington, D.C.What:BTF brings its nationwide initiative, the Road to Early Detection Sponsor-A-City ...
Breaking Medicine Technology: