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Building a biochemistry lab on a chip
Date:2/12/2013

plications that require parallel, time-, and space multiplex reactions on a single integrated circuit. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products.

"This technology makes it possible to do cell lysing and nucleic acid amplification reactions within these individual droplets the droplets are the reaction vessels or cuvettes that can be individually heated," Salm added.

"We also demonstrate that ssDNA probe molecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection," said Bashir, who is director of the Micro and Nanotechnology Laboratory at Illinois. "This platform enables many applications in droplets including hybridization of low copy number DNA molecules, lysing of single cells, interrogation of ligandreceptor interactions, and rapid temperature cycling for amplification of DNA molecules.

"Notably," Bashir added, "our miniaturized heater could also function as dual heater/sensor elements, as these silicon-on-insulator nanowire or nanoribbon structures have been used to detect DNA, proteins, pH, and pyrophosphates.

By using microfabrication techniques and incorporating the unique design of transistor-based heating with individual reaction volumes, 'laboratory-on-a-chip' technologies can be scaled down to 'laboratory-on-a-transistor' technologies as sensor/heater hybrids that could be used for point-of-care diagnostics."


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Contact: Rashid Bashir
rbashir@illinois.edu
217-333-3097
University of Illinois College of Engineering
Source:Eurekalert

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