Navigation Links
Yeast Protein Production System Features High Yields and One-Step,,,Purification

Figure 5

Conclusions

Stratagene's new ESP yeast protein expression and purification system provides protein production in a eukaryotic organism, retaining many posttranslational modifications of recombinant proteins. The main advantage of the ESP system over other eukaryotic expression systems is quick, easy expression and purification of recombinant proteins. The S. pombe system offers highyield production with options for either inducible or constitutive expression. The kit includes easytotransform E. coli and yeast competent cells, glutathione agarose beads for protein purification, yeast media and timesaving, wellcharacterized procedures.

Acknowledgments

We thank Paul Russell and Janet Letherwood of the Research Institute of Scripps Clinic for discussions and suggestions; Joe Sorge, Peter Vaillancourt, ChaoFeng Zheng, Rebecca Mullinax, Kerstien Padgett and other members of the research and development department at Stratagene for assistance and suggestions; Bryan Macilko and Allison Fowler for media preparations.

REFERENCES

  1. Sipiczki, M. (1989) In Molecular Biology of Fission Yeast (A. Nisim,P. Young, and B.F. Johnson, eds). Academic Press, San Diego, Calif.

  2. Smith, D.B., and Johnson, K.S. (1988) Gene 67: 3140.

  3. Maundrell, K. (1990) J. Biol. Chem. 265: 1085710864.

  4. Forsburg, S. (1993) Nucleic Acids Res. 21: 29552956.

  5. Hopp, H.P., et al. (1988) Biotechnology 6: 12041210.


'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:5/22/2015)... , May 22, 2015 According ... Product (1D & 2D Gel Electrophoresis, Agarose & Polyacrylamide ... Reagents, Informatics), by Application & by End User - ... Market is expected to reach around $1.98 Billion by ... period 2015 to 2020. Browse 226 ...
(Date:5/22/2015)... Knowledgent , the data and ... the potential of big data analytics in the life ... to help life sciences and healthcare organizations understand the ... critical business insights. , The life sciences and healthcare ... by patient profiling, compliance and regulatory requirements, and scientific ...
(Date:5/21/2015)... 2015 /PRNewswire/ - SQI Diagnostics Inc. ("SQI" or the ... develops and commercializes proprietary technologies and products for advanced ... for the second fiscal quarter ended March 31, 2015. ... to advance business with our existing customers and to ... and CEO of SQI. "The delivery of a fully ...
(Date:5/21/2015)... 21, 2015 Specialty Pharmacy Times ... been admitted to BPA Worldwide as a business ... track audience data for Specialty Pharmacy Times based ... “By becoming a member of BPA Worldwide, Specialty ... our clients with the most reliable, unequaled data,” ...
Breaking Biology Technology:Electrophoresis Market Worth $1.98 Billion by 2020 2Electrophoresis Market Worth $1.98 Billion by 2020 3Electrophoresis Market Worth $1.98 Billion by 2020 4New White Paper Explores the Potential of Big Data Analytics in Life Sciences and Healthcare 2SQI Diagnostics Reports Second Quarter Financial Results 2SQI Diagnostics Reports Second Quarter Financial Results 3SQI Diagnostics Reports Second Quarter Financial Results 4SQI Diagnostics Reports Second Quarter Financial Results 5SQI Diagnostics Reports Second Quarter Financial Results 6SQI Diagnostics Reports Second Quarter Financial Results 7SQI Diagnostics Reports Second Quarter Financial Results 8Specialty Pharmacy Times Joins BPA Worldwide 2
... the flow of light is essential in today,s telecommunications-based ... Laboratory of Photonics and Quantum Measurements have discovered a ... discovery, they built a device in which a beam ... controlled by a second, stronger light beam. The device ...
... Rensselaer Polytechnic Institute Professor James Jian-Qiang Lu ... technical achievements toward the design and realization of ... in the Department of Electrical, Computer, and Systems ... prestigious William D. Ashman Achievement Award for 2010 ...
... ANNAPOLIS, Md., Nov. 11, 2010 PharmAthene, Inc. (NYSE ... medical countermeasures against biological and chemical threats, today reported ... nine months ended September 30, 2010. For ... $6.2 million compared to $6.8 million in the same ...
Cached Biology Technology:All-optical transistor 2Rensselaer Polytechnic Institute professor James Lu garners award for research on 3-D computer chips 2PharmAthene Reports Third Quarter 2010 Financial and Operational Results 2PharmAthene Reports Third Quarter 2010 Financial and Operational Results 3PharmAthene Reports Third Quarter 2010 Financial and Operational Results 4PharmAthene Reports Third Quarter 2010 Financial and Operational Results 5PharmAthene Reports Third Quarter 2010 Financial and Operational Results 6PharmAthene Reports Third Quarter 2010 Financial and Operational Results 7
(Date:4/14/2015)...  HYPR Corp. today announced it has joined ... tm , an industry consortium transforming online authentication ... technology and collaborate to deliver open specifications for ... and private, and easier to use. ... sensitive user information and eliminates the dependency on ...
(Date:4/10/2015)... -- Research and Markets ( http://www.researchandmarkets.com/research/cjk4gb/security ) ... Profiles - NEC" report to their offering. ... continue to supply a range of IT security management ... focus on the development of a Big Data and ... the Asia-Pacific region is a ...
(Date:4/2/2015)... 2, 2015 In the ... far outperforms the strongest competitors, showing outstanding performance ... the system is capable of a throughput of ... up to be ten times faster than most ... performance.      (Photo: http://photos.prnewswire.com/prnh/20150402/738673-a ...
Breaking Biology News(10 mins):HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3NEC Security Competitive Profile 2015 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3
... (October 5, 2010) -- Protein molecules inside cells ... forces exerted by all the other molecules in ... models are necessarily built around much greater driving ... Journal of Chemical Physics investigates the ...
... State University researchers have discovered a rare oasis of life ... bottom of Yellowstone Lake. A colony of moss, worms ... the water is inky black, about 90 degrees Fahrenheit, and ... in the September issue of Geobiology. The vent is ...
... Oct. 4, 2010 -- Supercomputer simulations at the Department of ... nucleic acids could have contributed to the origins of life. ... who directs ORNL,s Center for Molecular Biophysics and holds a ... to probe an organic chemical reaction that may have been ...
Cached Biology News:Montana State team finds rare oasis of life on floor of Yellowstone Lake 2Montana State team finds rare oasis of life on floor of Yellowstone Lake 3Montana State team finds rare oasis of life on floor of Yellowstone Lake 4Powerful supercomputer peers into the origin of life 2
... (OSM) is a pleiotropic cytokine produced by ... cells. OSM inhibits the growth of some ... smooth muscle and Kaposis sarcoma cell proliferation, ... density lipoprotein receptor expression on hepatoma cells. ...
... trinor Prostaglandin F2α isopropyl ester (15(R)-17-phenyl ... latanoprost-related isomer containing both a double ... (β) hydroxyl group at C-15. Similar ... ester is a potential impurity in ...
ACS Reagent Grade C 19 H 14 O 5 S Formula Weight: 354.38 PRODUCT SPECIFICATIONS Form: Rust/red crystalline powder Clarity of Solution: Passes test Visual Transition Interval: pH 6.8 - Yellow pH 8.2...
... Mouse Anti Nop1p/Fibrillarin (monoclonal) ... nucleolar protein of bakers yeast, Saccharomyces ... is essential for yeast viability and ... human homologue of Nop1p is fibrillarin ...
Biology Products: