Navigation Links
Yeast Protein Production System Features High Yields and One-Step,,,Purification

Figure 5

Conclusions

Stratagene's new ESP yeast protein expression and purification system provides protein production in a eukaryotic organism, retaining many posttranslational modifications of recombinant proteins. The main advantage of the ESP system over other eukaryotic expression systems is quick, easy expression and purification of recombinant proteins. The S. pombe system offers highyield production with options for either inducible or constitutive expression. The kit includes easytotransform E. coli and yeast competent cells, glutathione agarose beads for protein purification, yeast media and timesaving, wellcharacterized procedures.

Acknowledgments

We thank Paul Russell and Janet Letherwood of the Research Institute of Scripps Clinic for discussions and suggestions; Joe Sorge, Peter Vaillancourt, ChaoFeng Zheng, Rebecca Mullinax, Kerstien Padgett and other members of the research and development department at Stratagene for assistance and suggestions; Bryan Macilko and Allison Fowler for media preparations.

REFERENCES

  1. Sipiczki, M. (1989) In Molecular Biology of Fission Yeast (A. Nisim,P. Young, and B.F. Johnson, eds). Academic Press, San Diego, Calif.

  2. Smith, D.B., and Johnson, K.S. (1988) Gene 67: 3140.

  3. Maundrell, K. (1990) J. Biol. Chem. 265: 1085710864.

  4. Forsburg, S. (1993) Nucleic Acids Res. 21: 29552956.

  5. Hopp, H.P., et al. (1988) Biotechnology 6: 12041210.


'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:9/30/2014)... ROCKVILLE, Md. , Sept. 30, 2014 /PRNewswire/ ... "RegeneRx") today announced that it has received a ... Aging by Actin-Sequestering Peptides.  This patent includes claims ... and numerous other actin-sequestering peptides for these purposes.  ... About RegeneRx Biopharmaceuticals, Inc. ( www.regenerx.com ) ...
(Date:9/30/2014)... OriginOil Inc. (OTC/QB: OOIL), developer of ... clean up large quantities of water, announced today that ... on the launch of the company's CLEAN-FRAC™ industrial grade ... partner STW Resources . , Immediately upon startup, ... (bpd) and achieved a 99.8% reduction in Turbidity on ...
(Date:9/30/2014)... iLab Solutions announced the results of ... which focuses on core facility operations, growth and utilization ... today. , In its 4th year running, a number ... the year-over-year decrease in the percentage of revenue that ... feeling the constraints of this trend. When asked, “What ...
(Date:9/30/2014)... 30, 2014 SoundConnect, ... company is dedicated to supporting business communication solutions, ... Julie Power, a carrier services specialist ... solutions stays connected and productive at all times. ... movement of businesses to cloud communications services. ...
Breaking Biology Technology:RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2OriginOil, Inc. Report to Shareholders: Blue Gold in West Texas 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 3SoundConnect Expands Carrier Services Division 2
... DIEGO, Aug. 8 Halozyme Therapeutics, Inc.,(Nasdaq: ... targeting the extracellular matrix, today reported financial,results for ... 2008., "As we,ve previously stated, we continue ... programs that target large markets. Now we are,beginning ...
... -- China Sky One,Medical, Inc. ("China Sky One ... a,manufacturer, marketer and distributor of pharmaceutical, medicinal and,diagnostic ... conduct a,conference call at 10:00 a.m. Eastern Time ... 2008 financial results., Joining Mr. Yan-qing Liu, ...
... 8 Insmed Inc. (Nasdaq:,INSM), a developer of follow-on biologics and ... 30, 2008., Recent Company Highlights, -- Follow-on Biologics ... bioequivalence of INS-19, the ... factor (G-CSF), to Neupogen(R), an FDA-approved G-CSF product for ...
Cached Biology Technology:Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 2Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 3Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 4Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 5Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 6Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 7Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 8Halozyme Therapeutics Reports 2008 Second Quarter Financial Results 9China Sky One Medical, Inc. Announces Conference Call to Discuss Second Quarter 2008 Results 2China Sky One Medical, Inc. Announces Conference Call to Discuss Second Quarter 2008 Results 3Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 2Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 3Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 4Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 5Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 6Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 7Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 8Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 9Insmed Announces Financial Results for Second Quarter and Six-Months Ended June 30, 2008 10
(Date:9/30/2014)... conducted by the Ocean Health Index rates the ... overall health. In addition, for the first time, ... ocean regions beyond national jurisdiction (high-seas areas) ... safeguarding biodiversity and providing sustainable food sources. , ... a partnership led by scientists from UC Santa ...
(Date:9/30/2014)... Ecker, a Salk professor and Howard Hughes Medical ... have been named recipients in the 2014 round ... (NIH) through the BRAIN (Brain Research through Advancing ... The grant, announced September 30, provides more than ... over three years. , The BRAIN Initiative, launched ...
(Date:9/30/2014)... almost any aquatic environment on Earth, but when the ... pushed to the limit. The amount of time needed ... how different species cope in the future, reveals a ... published in the scientific journal Proceedings of the ... thanks to increasing levels of greenhouse gases in the ...
Breaking Biology News(10 mins):Rating the planet's oceans 2Rating the planet's oceans 3Salk scientists receive $3 million for BRAIN Initiative grant 2Shape up quickly -- applies to fish, too! 2
... When neurons started dying in Clive Svendsen,s lab dishes, ... cells the same type lost in patients with ... that the University of Wisconsin-Madison stem cell biologist had ... lab, using stem cells derived from a patient. ...
... left Africa over 60,000 years ago in a migration that ... population that exist outside Africa today. Now, researchers ... that exodus. By tracing variations in the X chromosome and ... probably outnumbered women in that migration. The scientists expect that ...
... Deep inside the Frasassi cave system in Italy and more ... ropes of microbes growing in the cold water, according to ... microbiology paradise. Many different types of organisms live in the ... professor of geosciences. "We are trying to map which organisms ...
Cached Biology News:Patient-derived induced stem cells retain disease traits 2Patient-derived induced stem cells retain disease traits 3Unusual microbial ropes grow slowly in cave lake 2
...
... is the research-grade spectrofluorometer with a minimum ... S/N ratio 200 or greater for the ... especially designed to keep in mind in ... kinetics, stopped-flow, titration, or anisotropic measurements. ,The ...
... Microarrays designed for genome-wide microRNA expression ... and powerful Paraflo microfluidic on-chip synthesis ... part of our comprehensive microRNA Expression ... The Arabidopsis microRNA Microarray contains ...
... Microarrays designed for genome-wide microRNA expression profiling ... Paraflo microfluidic on-chip synthesis platform. These microarrays ... microRNA Expression Profiling Service. Probe ... all known cow microRNAs (108 unique mature ...
Biology Products: