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Yeast Protein Production System Features High Yields and One-Step,,,Purification

Figure 5

Conclusions

Stratagene's new ESP yeast protein expression and purification system provides protein production in a eukaryotic organism, retaining many posttranslational modifications of recombinant proteins. The main advantage of the ESP system over other eukaryotic expression systems is quick, easy expression and purification of recombinant proteins. The S. pombe system offers highyield production with options for either inducible or constitutive expression. The kit includes easytotransform E. coli and yeast competent cells, glutathione agarose beads for protein purification, yeast media and timesaving, wellcharacterized procedures.

Acknowledgments

We thank Paul Russell and Janet Letherwood of the Research Institute of Scripps Clinic for discussions and suggestions; Joe Sorge, Peter Vaillancourt, ChaoFeng Zheng, Rebecca Mullinax, Kerstien Padgett and other members of the research and development department at Stratagene for assistance and suggestions; Bryan Macilko and Allison Fowler for media preparations.

REFERENCES

  1. Sipiczki, M. (1989) In Molecular Biology of Fission Yeast (A. Nisim,P. Young, and B.F. Johnson, eds). Academic Press, San Diego, Calif.

  2. Smith, D.B., and Johnson, K.S. (1988) Gene 67: 3140.

  3. Maundrell, K. (1990) J. Biol. Chem. 265: 1085710864.

  4. Forsburg, S. (1993) Nucleic Acids Res. 21: 29552956.

  5. Hopp, H.P., et al. (1988) Biotechnology 6: 12041210.


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