Navigation Links
Yeast Protein Production System Features High Yields and One-Step,,,Purification

ides sufficient thiamine to repress the nmt1 promoter. Expression is induced by harvesting the cells and growing them in EMM broth without thiamine for approximately 18 hours.

Figure 2

The chicken calmodulin gene was inserted into the pESP-1 vector and expressed in S. pombe. Figure 2 shows the cell lysate samples derived from repressed and induced cells containing chicken calmodulin. When cells were grown under induced conditions, the crude lysate shows a dominant band at the size expected for a GST-calmodulin fusion protein (43 kDa) (figure2, lane 4). In the lysate of cells grown under repressed conditions, this band was absent. (Figure 2, lane 1) When the samples were subjected to one-step purification of the GST fusion protein by using GST affinity resin, the induced GST-calmodulin fusion protein was depleted in the glutathione resin flowthrough sample (Figure 2, lane 5) and was eluted to near homogeneity in the glutathione elution fraction (Figure 2, lane 6). No detectable GST fusion protein was observed when lysate derived from repressed cells was subjected to the same purification procedure (Figure 2, lane 3). Figure 3 shows Western blot analysis of the GST-calmodulin fusion protein in cell lysates derived from repressed and induced cells. The fusion protein was not detected in cells when the promoter was repressed but was abundant in cells when the promoter was induced. We have also observed that constitutive, high-level expression of GST fusion proteins is possible by inoculating the cells directly into the induction medium (EMM), which significantly simplifies the overall procedure. However, proteins
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:10/20/2014)... October 20, 2014 Convey Computer ... a team from Iowa State University won first place ... Using a Convey HC-2ex, the team’s solution achieved the ... faster than the second place finisher. , Experts from ... upon the month long challenge, using a variety of ...
(Date:10/20/2014)... Oct. 20, 2014 PureTech , a ... big healthcare problems, announced today the closing of ... participation from Invesco Perpetual, a $120 Billion group ... drive PureTech,s existing pipeline forward and to advance ... the scientific creativity to really go for the ...
(Date:10/19/2014)... 2014 OCTOBER 20-22, 2014: ... ABIM will take place at the ... ABIM 2014 is now available at ... companies and organizations from all over the ... on the latest products and developments on ...
(Date:10/19/2014)... The report "Chocolate, Cocoa Beans, Lecithin, ... Geography Trend and Forecast (2011 - 2016)," analyzes ... and studies the major market drivers, restraints, and opportunities ... Asia. , The global chocolate market is expected to ... 2016 at an estimated CAGR of 2.7% from 2011 ...
Breaking Biology Technology:Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4
... 21-1/2 hours of great entertainment and powerful appeals, the final ... Lewis MDA Telethon which raised $58,919,838 in contributions and ... Muscular Dystrophy Association.   "I,m heartened by the ... they themselves are likely struggling financially," said Lewis.  "People see ...
... COLUMBIA, Md., Sept. 3 The Maryland Stem Cell ... Stem Cell Research Symposium in partnership with the National ... Standards and Technology (NIST) on Wednesday, Sept. 22, 2010. ... Groundbreaking Life Science Research," will be held at the ...
... - 2015) -... -- DALLAS, Texas, September 3, 2010 ... ... ... Results are keyed by longUrl, so we need to grab the first one. for (var r in data.results) ...
Cached Biology Technology:MDA Telethon Exceeds Expectations 2Maryland Stem Cell Research Commission to Host Third Annual Maryland Stem Cell Research Symposium 2Maryland Stem Cell Research Commission to Host Third Annual Maryland Stem Cell Research Symposium 3New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 2New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 3New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 4New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 5New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 6New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 7New Report - U.S. Digestive Health Ingredients Markets (2010 - 2015) - MarketsandMarkets 8
(Date:10/15/2014)... have linked increased resistance to bacterial pneumonia in female ... hormone estrogen. , Females are naturally more resistant to ... scientists has shown that increased resistance to bacterial pneumonia ... oxide synthase 3 (NOS3). They also show that this ... female sex hormone estrogen. , The team, lead by ...
(Date:10/15/2014)... awarded the 44th Rosenstiel Award for Distinguished Work in ... exploring the mechanisms of genomic instability and its implications ... the second alumnus to win the Rosenstiel Award; the ... , Alt is the Charles A. Janeway Professor of ... and an investigator at the Howard Hughes Medical Institute ...
(Date:10/14/2014)... tumors scavenge and hoard copper that is an essential ... a fatal weakness. , Researchers at Duke Medicine ... by delivering a trove of copper along with a ... the mineral, leaving non-cancer cells healthy. , The combination ... other uses, could soon be tested in clinical trials ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Prostate cancer's penchant for copper may be a fatal flaw 2
... would overheat and die. In a recent paper in the journal ... team of researchers explored the ultimate origin of this sticky, stinky ... his team used a system to make all of the sweat ... green fluorescent protein (GFP), which is visible under ultraviolet light. ...
... the first evidence that coral itself may play an ... discovered that the coral animalnot just its algal symbiontmakes ... in many ways, ranging from cellular protection in times ... clouds to form. These findings have been published ...
... combination of the herbicide atrazine and a fungal disease ... a University of South Florida laboratory, which has been ... Biologist Jason Rohr said the new findings show that ... when the frogs were challenged with a chytrid fungus, ...
Cached Biology News:USC researcher learns how to break a sweat 2Coral chemicals protect against warming oceans 2Early-life exposure of frogs to herbicide increases mortality from fungal disease 2
... have been many modifications to the original formulas ... solutions still play an important role in tissue,culture. ... pH and osmotic balance in the medium and ... inorganic ions, is as valuable today as when ...
Request Info...
... LXQ linear ion trap mass spectrometer enables ... laboratory., The LXQ linear ion trap mass ... proteomic applications, delivering the fast cycle time ... ion trap at an attractive price. ...
... Safe DNA gel stain was developed specifically for ... bromide for staining DNA in agarose or acrylamide ... mutagenic than ethidium bromide but SYBR Safe stain's ... bromide. SYBR Safe stain comes as a premixed ...
Biology Products: