Navigation Links
Yeast Protein Production System Features High Yields and One-Step,,,Purification

s of the S. pombe host strain and reagents for protein induction and purification.

The pESP-1 Expression Vector

figure 1

Designed for expression in S. pombe, the pESP-1 vector (figure 1) is composed of the following parts: (1) a ColE1 origin of replication and an ampicillin resistance (Ampr) fragment, which allow vector replication and antibiotic selection in E. coli; (2) the ars1 fragment, which provides an origin of replication for the vector to replicate autonomously in S. pombe; (3) a LEU2-d gene from Saccharomyces cerevisiae, which serves as a selection marker for transforming the expression vector into S. pombe cells (strain SPQ01) and (4) the expression cassette, which contains the S. pombe nmt1 promoter, a translational start site, a GST protein-tag sequence, a multiple cloning site and the nmt1 transcription termination signal. Insertion of the gene of interest into the multiple cloning site results in an N-terminal fusion with the GST peptide, which facilitates one-step purification of the GST fusion protein.2

Figure 3

The nmt1 promoter of S. pombe is tightly repressed in the presence of thiamine (vitamin B1) in the growth medium and is highly activated upon its removal.3 When activated, the nmt1 promoter has been shown to be one of the strongest promoters in S. pombe.4 For this reason, the nmt1 promoter has been widely used to study gene function in S. pombe. Figure 3 shows Western blot analysis of the GST-calmodulin fusion protein in the pESP-1 vector that has been expressed in cells grown under
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. A New C-Terminal GST Vector for Protein Production in S. pombe
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
10. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:10/19/2014)... -- NextCODE Health, which enables clinicians and researchers to use ... announced the launch of its new genomic data analysis and ... Society of Human Genetics (ASHG) annual meeting in ... apply for free beta access, visit www.nextcode.com . A ... viewed here . The Exchange: ...
(Date:10/19/2014)... Asia-Pacific Speech Analytics market report defines and segments the ... of revenue. This market is estimated to grow from ... at a Compound Annual Growth Rate (CAGR) of 23.9% ... of the Asia-Pacific Speech Analytics market report to get ... provides a glimpse of the segmentation in the market, ...
(Date:10/19/2014)... October 19, 2014 The report ... & Homeland Security), Technology, Payload, Type & by ... segments the global unmanned ground vehicle market into ... forecasting of market sizes. The report also identifies ... opportunities impacting it along with the adoption trends. ...
(Date:10/18/2014)... The Asia-Pacific Bio-based Advanced Phase Change ... advanced phase change material market in Asia-Pacific with ... the TOC of the Asia-Pacific Bio-based Advanced Phase ... of the in-depth analysis provided. This also provides ... bio-based advanced phase change material market, and is ...
Breaking Biology Technology:The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 4
... , , Phase Lock Gel may be used in ... total RNA from cell and tissue samples. Increased yields are , ... , without interphase contamination. Below is a protocol outlining ... Reagent and Phase Lock Gel-Heavy. , For further details ...
... , , , , , , ... Eppendorf 5 Prime, Inc. , , , ... It is not convenient in all instances to have blood drawn for gDNA extraction. ... to extract gDNA from , saliva with only slight modifications ...
... , , , , , ... 5 Prime, Inc. , , , , , ... are the least invasive way of collecting gDNA from humans, , ... pathogens. Buccal swabs are quickly becoming the method of choice when , ...
Cached Biology Technology:Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock , Gel-Heavy 2Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock , Gel-Heavy 3Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini , Kit 2Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini , Kit 3Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini , Kit 4Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini , Kit 5Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini , Kit 6Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood , Mini Kit 2Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood , Mini Kit 3Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood , Mini Kit 4Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood , Mini Kit 5
(Date:10/15/2014)... our ability to assess the pandemic risk from strains ... we must not allow ourselves to become complacent that ... international consortium of scientists. , Influenza pandemics arise when ... yet to develop widespread immunity – spreads in the ... the past 100 years, the worst of which – ...
(Date:10/15/2014)... NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) ("NXT-ID" or ... commerce market releases photos and video of the recent opening bell ... th . Gino Pereira , CEO of ... Mr. Chad A. Verdi rang the bell.  ... and employees "for their work and dedication in bringing the world,s ...
(Date:10/14/2014)... been millions of years since T. rex ... Ohio University scientists is breathing life back into dinosaurs ... snouts. The research has important implications for how dinosaurs ... enhance the sense of smell and cool their brains. ... University doctoral student Jason Bourke, lead author of the ...
Breaking Biology News(10 mins):Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 3Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3
... Triticum aestivum ) is one of the "big three" globally ... people. Fully 35% of the world,s 7 billion people depend ... of scientists, including a group from Cold Spring Harbor Laboratory ... genome. The study reveals the evolution of bread ...
... conducting a detailed study of the potential environmental and ... drilling process known as fracking. In hydraulic fracturing, ... deep underground to break apart rock and free trapped ... decades, recent technical advances have helped unlock vast stores ...
... Picture two charged particles in a vacuum. Thanks to ... force these particles exert upon one another, and therefore ... simple medium say, water and the calculation ... water, which in turn may slow, speed, or otherwise ...
Cached Biology News:Bread wheat's large and complex genome is revealed 2Bread wheat's large and complex genome is revealed 3Fracking in Michigan: U-M researchers study potential impact on health, environment, economy 2Fracking in Michigan: U-M researchers study potential impact on health, environment, economy 3Fracking in Michigan: U-M researchers study potential impact on health, environment, economy 4Outside a vacuum: Model predicts movement of charged particles in complex media 2
Large-capacity refrigerated incubator chamber holds up to 4 independently-operated shakers. Programmable heat, refrigeration, humidifcation are standard. Photosynthetic operation, optional....
...
... Advion's NanoMate 100 robotic system and ... full advantage of the capabilities of electrospray ... the NanoMate with MS allows users to ... small molecule and peptide mapping applications, or ...
... Mixes are optimised for SYBR Green I assays ... Quantitative PCR, with the exception of template and ... optimal levels of active SYBR Green I dye ... reaction buffer that enables detection of low copy ...
Biology Products: