Navigation Links
XL10-Gold Cells Supplied in New Single-Reaction Format

hat a heat pulse of 54C for 60 seconds was optimal to achieve an efficiency of 1 x 109 transformants/g of supercoiled DNA in the single-tube format.

The XL10-Gold strain exhibits a faster growth rate and produces larger colonies than other commonly used hosts like DH5a and XL2-Blue. To quantitate this difference, we performed a standard growth curve for these strains harboring the pBluescript plasmid. Results in Figure 1 show that XL10-Gold cells grew demonstrably faster than DH5a and XL2-Blue cells.

Figure 1

Conclusions

Stratagene now provides SoloPack Gold supercompetent cells (1 x 109 transformants/g supercoiled DNA) and competent cells (1 x 108 transformants/g supercoiled DNA) created from our XL10-Gold strain in a handy single-reaction format. The XL10-Gold strain exhibits the Hte phenotype for high transformation efficiency and provides robust growth to produce larger colonies than other commonly used hosts. Combine the XL10-Gold strain with the SoloPack format, and streamline the transformation process while retaining high efficiency.

Genotype

XL10-Gold strain: Tetr D(mcrA)183 D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F proAB lacI qZDM15 Tn10 (Tetr) Amy Camr]

The XL10-Gold strain is tetracycline and chloramphenicol resistant.

REFERENCES
  1. Jerpseth, B., Callahan, M., and Greener, A. (1997) Strategies 10: 37-38.

  2. Jerpseth, M., et al. (1998) Strategies 11: 3-4.


  3. '"/>


Source:


Page: All 1 2 3 4

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
6. Simple Isolation of RNA from Tissue and Cultured Cells
7. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
8. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Gold Cells Supplied New Single Reaction Format

(Date:8/19/2014)... (PRWEB) August 19, 2014 ... to raise $200,000 to fund a corporate lab ... , This development is a critical component ... of care to the implementation of personalized medicine ... of practicing medicine. , Indiegogo contributions will support ...
(Date:8/19/2014)... La Jolla, CA (PRWEB) August 19, 2014 ... the toll Parkinson’s disease can take on an individual. ... tremoring extremities, decreased facial expression, problems swallowing and severe ... due to the dying off of dopamine producing neurons ... retain themselves from a disease that slowly and progressively ...
(Date:8/19/2014)... Research and Markets ... "Spectroscopy Equipment and Accessories - Global Strategic ... This report analyzes the ... in US$ Thousands by the following Product ... NMR-EPR Spectrometers, Near Infrared (NIR) Spectrometers, IR ...
(Date:8/19/2014)... 2012, the European Union (EU) mandated use of the ICH-recommended ... – responding to the increasing need to understand and manage ... many regions, the main objectives of the PBRER are to:, ... of new or emerging information on the risks of a ... potential benefits of a drug; and , Allow ...
Breaking Biology Technology:Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 2As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2Top Five Best Practices for Developing Your Periodic Benefit Risk Evaluation Report (PBRER), New Webinar Hosted by Xtalks 2
... SOUTH SAN FRANCISCO, Calif. , April 29 ... creating new therapeutics discovered using cellular reprogramming and directed ... been awarded a three-year $1.5 million "Basic ... (CIRM). The grant will be used to fund the ...
... April 29 HEALTHeCAREERS Network , North America ,s ... , , ... , , , ... Angiography and Interventions (SCAI) recently joined the Network, launching the SCAI ...
... CHICAGO , April 29  Scotland ... attracted many of the world,s leading pharma and biotech ... May 3-6 in Chicago .  Heading ... Scottish research and the nation,s unique integrated industry approach ...
Cached Biology Technology:iPierian Awarded Basic Biology Grant by California Institute of Regenerative Medicine to Study Cellular Reprogramming 2iPierian Awarded Basic Biology Grant by California Institute of Regenerative Medicine to Study Cellular Reprogramming 3Healthcare Web site Expands Reach to Cardiology Professionals 2Healthcare Web site Expands Reach to Cardiology Professionals 3Scotland Announces New Life Science Leadership and Investments at BIO 2010 2Scotland Announces New Life Science Leadership and Investments at BIO 2010 3Scotland Announces New Life Science Leadership and Investments at BIO 2010 4
(Date:8/20/2014)... by researchers at the University of Missouri School of ... life-threatening heart condition caused by muscular dystrophy. , ... Ph.D., the leading author of the study and assistant ... of Molecular Microbiology and Immunology. "This is just a ... a treatment for people with this devastating heart condition, ...
(Date:8/20/2014)... State University researchers have developed methods for electronically manipulating ... electrical signals moths use to control those muscles. The ... moths, or "biobots," for use in emergency response. , ... we can control the movement of moths for use ... Dr. Alper Bozkurt, an assistant professor of electrical and ...
(Date:8/20/2014)... and POINT ROBERTS, Washington , August ... a Key Role in Addressing Security Concerns in the Payment ... global news source covering leading sectors including biometrics, issues commentary ... Jason Peaslee , Managing Partner at Thrive ... Gino Pereira , CEO of NXT-ID, Inc. (OTCBB: NXTD) ...
Breaking Biology News(10 mins):Gene therapy protects mice from lethal heart condition, MU researchers find 2Research paves way for development of cyborg moth 'biobots' 2Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 2Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 3Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 4Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 5Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 6Digital Wallets for a Digital World - the Players and the Concerns for Consumer Adoption 7
... explore one of the most critical health/environment intersections how ... diabetes and contribute to the problems of obesity Michigan ... by the Environmental Protection Agency. A five-year, $8 million ... Professor of pathobiology and diagnostic investigation in the College of ...
... Starting rehabilitation sooner following knee arthroplasty surgery could ... physical therapy within 24 hours of surgery can improve ... well as cut hospital stays, according to new research ... SAGE. Mindful of the trend towards discharging patients ...
... CA "Pluripotent" stem cellswhich have the potential to mature ... for their role in treating a vast array of human ... a team of Scripps Research Institute scientists has created a ... for researchers to determine whether their cell lines are normal ...
Cached Biology News:Hold your breath: Air pollution plays role in cardiac, metabolic diseases 2Rehabilitation within a day of knee replacement pays off 2Scripps Research scientists develop new test for 'pluripotent' stem cells 2