solopack Gold cells reduce the time and effort needed for transformationswithout compromise!
Anna Waesche Jonathan Goodwin Amy Duncan Bruce
Jerpseth Alan Greener
Stratagene introduces competent cells from the XL10-Gold strain* in an easy-to-use, single-reaction format that yield the supercompetent efficiency of 1 x 10 9 transformants/g of supercoiled DNA or the competent efficiency of 1 x 10 8 transformants/g of supercoiled DNA. The new Epicurian Coli SoloPack Gold supercompetent and competent cells exhibit the Hte phenotype for high transformation efficiency and exhibit robust growth to produce large colonies. The SoloPack format saves time by providing a convenient transformation format.
Transformation of competent E. coli cells is crucial for cloning a gene, expressing a protein, or constructing a plasmid library. In many of these instances, transformation is the rate-limiting step. Working with an E. coli strain that efficiently transforms ligated DNA in a convenient format is, therefore, desirable. Stratagene developed the XL10-Gold strain, containing the Hte phenotype,** to produce the highest transformation efficiencies for ligated DNA. This phenotype is observed in cells that carry an uncharacterized mutation that has been shown to improve the efficiency of transforming ligated plasmids and large DNA molecules relative to strains of equal competency (as measured by supercoiled pUC DNA transformation).1,2
In any transformation reaction, competent cells must be thawed and aliquotted
into Falcon 2059 polypropylene reaction tubes. To speed up the
transformation process, we designed the SoloPack format so that the entire
transformation reaction, including post heat-shock expression, is performed in a
single tube. Th