Navigation Links
XL10-Gold Cells Supplied in New Single-Reaction Format


solopack Gold cells reduce the time and effort needed for transformationswithout compromise!

Anna Waesche Jonathan Goodwin Amy Duncan Bruce Jerpseth Alan Greener
Stratagene

Stratagene introduces competent cells from the XL10-Gold strain* in an easy-to-use, single-reaction format that yield the supercompetent efficiency of 1 x 10 9 transformants/g of supercoiled DNA or the competent efficiency of 1 x 10 8 transformants/g of supercoiled DNA. The new Epicurian Coli SoloPack Gold supercompetent and competent cells exhibit the Hte phenotype for high transformation efficiency and exhibit robust growth to produce large colonies. The SoloPack format saves time by providing a convenient transformation format.

Transformation of competent E. coli cells is crucial for cloning a gene, expressing a protein, or constructing a plasmid library. In many of these instances, transformation is the rate-limiting step. Working with an E. coli strain that efficiently transforms ligated DNA in a convenient format is, therefore, desirable. Stratagene developed the XL10-Gold strain, containing the Hte phenotype,** to produce the highest transformation efficiencies for ligated DNA. This phenotype is observed in cells that carry an uncharacterized mutation that has been shown to improve the efficiency of transforming ligated plasmids and large DNA molecules relative to strains of equal competency (as measured by supercoiled pUC DNA transformation).1,2

In any transformation reaction, competent cells must be thawed and aliquotted into Falcon 2059 polypropylene reaction tubes. To speed up the transformation process, we designed the SoloPack format so that the entire transformation reaction, including post heat-shock expression, is performed in a single tube. Th
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
6. Simple Isolation of RNA from Tissue and Cultured Cells
7. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
8. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Gold Cells Supplied New Single Reaction Format

(Date:1/14/2014)... , Jan. 14, 2014 The largest international ... medicinal plants and therapeutic derivatives thereof has endorsed ... and researchers about the challenges of adulterated herb ... ) The Society for Medicinal ...
(Date:1/14/2014)... 2014 iLab Solutions, the leader in core ... Director of Product Strategy. In this role, Michelle will work ... to guide in the development of iLab products. Her main ... possible benefit to the scientific community by offering the most ...
(Date:1/14/2014)... WESTBOROUGH, Mass. , Jan. 14, 2014  RXi ... on discovering, developing and commercializing innovative therapies addressing ... announced that it received the Notice of Allowance ... on its unique self-delivering RNAi compounds (sd-rxRNA®), for ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
Breaking Biology Technology:World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... DIEGO, Jan. 3, 2012  Amylin Pharmaceuticals, Inc. (Nasdaq: ... Morgan Healthcare Conference in San Francisco on Monday, January 9, ... Daniel M. Bradbury, president and chief executive officer of ... presentation and break-out session will be webcast through the "Investors" ...
... Renowned biochemist Michael A. Marletta, PhD, assumed the post ... January 1, succeeding Richard A. Lerner, MD, who led ... (Photo:  http://photos.prnewswire.com/prnh/20120102/DC28698 ) "I,m excited ... knowledge in the biosciences and applying that knowledge to ...
... BioNeutral Group, Inc. (OTCBB: BONU), a specialty life ... received $400,000 in connection with the Preferred Stock ... ("Vinfluence"), New South Wales, Australia.  Pursuant to the ... initial closing and a second funding of $200,000, ...
Cached Biology Technology:Michael A. Marletta Takes Office as New President of Scripps Research Institute 2BioNeutral Group Receives $400,000 in Equity Financing 2BioNeutral Group Receives $400,000 in Equity Financing 3
(Date:4/23/2014)... who have had a stroke, often suffer motor deficits ... study conducted in Taiwan, that will be published in ... is currently freely available on-line as an unedited early ... one group of stroke victims had their own peripheral ... and a similar group did not, those who received ...
(Date:4/23/2014)... the energy landscape in the U.S., but in ... the energy industry, residents and agricultural interests over ... degraded water quality is a potential risk unless ... in the ACS journal Environmental Science & ... point out that a major criticism of extracting ...
(Date:4/23/2014)... Climate fiction, or simply cli-fi, is a newly coined term ... global warming. New research from University of Copenhagen shows how ... to simulate the potential consequences of climate change and imagine ... than scientific data on changes in the atmosphere; it is ... by the books we read and the films we see. ...
Breaking Biology News(10 mins):Autologous stem cell therapy improves motor function in chronic stroke victims 2How to avoid water wars between 'fracking' industry and residents 2Fiction prepares us for a world changed by global warming 2Fiction prepares us for a world changed by global warming 3
... as calcified, inert structures, but researchers at Columbia University ... important novel function of the skeleton. Theyve shown for ... organ that helps control our sugar metabolism and weight ... development of type 2 diabetes. The research, ...
... passed on from mother to child is decided very early ... ovary. In a cell division process that is unique to ... egg before it is fertilised. Using a powerful microscope, researchers ... how the molecular machinery functions that is responsible for chromosome ...
... Haven, Conn. It is not just whats in your genes, ... between species at least in yeast according to a ... Weve known for a while that the protein coding genes of ... author Michael Snyder , the Cullman Professor of Molecular ...
Cached Biology News:Research shows skeleton to be endocrine organ 2A unique arrangement for egg cell division 2Evolution is driven by gene regulation 2
This monoclonal antibody specifically recognizes Y. pestiscapsular F1 antigen. It does not cross-react with Y. pseudotuberculosisor Y. enterocolitica....
... was first identified as the cellular homolog of ... and Jun D have been shown to be ... which are involved in dimerization and DNA binding, ... transcriptional activation, diverge. Antigen: ...
IHC Zinc Fixative - , 1000 ml Consult technical datasheet for details....
... (IC) Fixation Buffer, used in conjunction ... 00-8333), can be used to fix ... intracellular staining of cytokines and other ... have been specially formulated for reducing ...
Biology Products: