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WHOLE GENOME SEQUENCING

entation of high throughput genomics, our background in building a highly scalable sequencing pipeline and our experience managing data from largescale sequencing projects enables us to offer complete genome sequencing with industry-leading quality and the highest level of sample and data integrity.

LIBRARY CONSTRUCTION STRATEGIES
Agencourt has experienced personnel dedicated to shotgun library construction and verification. We use unique library construction strategies to provide more random libraries with increased genome coverage which are customized for the specific needs of each project.

FINISHING AND ASSEMBLY
Agencourt offers high quality finishing. Our quality standards are among the highest in the industry, with error rates well below 1 in 10,000 nucleotides in the assembled contigs. Our finishing strategies can include custom primer walking or transposon insertion methodologies. We use both commercial and proprietary bioinformatics software packages for finishing and assembly including Phred, PHRAP and GAP 4. Paired end sequencing data allows us to resolve repeat sequences and to maximize the level of contig ordering.

ANNOTATION
Highly experienced annotation personnel have the ability to bring the project to full completion with in-depth sequence annotation including nucleotide and amino acid-based homology searches. Several automated annotation options are available including Ab initio gene prediction of genomic sequences, homology searches using BLAST algorithms, and protein domain searches using hidden Markov models.

PROTEIN-PROTEIN INTERACTION MAPPING
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