Navigation Links
Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells

evels of expression of exogenous proteins typical of transiently transfected cells. It was therefore necessary to optimize our transfection experiments in order to produce enough positively transfected cells for data analysis. In an attempt to overcome these difficulties, we compared the effectiveness of two liposome-based transfection reagents in SW13 cells using pEGFP-C3 as a control vector.

Transient Transfection of the pEGFP-C3 Vector into SW13 Cells

SW13 cells (8 x 104) were plated in a 24-well culture dish and allowed to attach overnight so that the cell density was between 60% and 80% confluent. In order to compare the relative efficiencies of the two liposome-mediated reagents, we used the manufacturers recommendations for concentration of reagent and DNA as a starting point for our experiments. Additional transfections were performed that used more or less of either the reagent or DNA. All procedures were performed according to the manufacturers recommendations, including the use of serum-free media for LipofectAMINE reagent-mediated transfections and serum-enriched media when adding LipoTAXI transfection reagent and DNA solutions to SW13 cells. Cells were exposed to the DNA-liposome complex for 5 hours at 37C in a humidified incubator with 5% CO2. Serum-containing medium was added to the cells, and the cells were incubated overnight. Cells were then fixed with 4% paraformaldehyde at room temperature for 25 minutes and rinsed with deionized, distilled water. Coverslips with attached cells were mounted in glycerol containing 2.5% 1,4-diazabicyclo(2.2.2)octane (DABCO) then viewed and photographed using a Nikon Microphot FX light microscope. Transfection efficiency was determined by counting fluorescent and total cells from six random fields for each condition.

Figure 1 shows fluorescent images of
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
4. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
5. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
6. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
7. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
8. Efficient Transfection of Neurospora Crassa
9. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:
(Date:12/19/2014)... 18, 2014 iLab Solutions, the global ... of Siri Bryant as the new Director of Institutional ... Core Implementations. These two new leadership positions were created ... by ensuring that iLab continues to meet the needs ... four years, iLab has been deployed at over 450 ...
(Date:12/19/2014)... BURLINGTON, Mass. , Dec. 18, 2014  Decision ... dental biomaterial market will grow moderately through ... implant procedures and growing awareness of dental biomaterials all ... underdeveloped markets such as China ... incomes will bring dental procedures within reach for a ...
(Date:12/17/2014)... December 16, 2014 The Activated ... 2013, which is why IBISWorld updated its original industry ... to benefit from an intensified focus on environmental policy. ... new emission standards for power plants and a range ... to IBISWorld Industry Analyst Sarah Kahn, “a range of ...
(Date:12/17/2014)... 17, 2014 Ipsen Biopharmaceuticals, Inc., ... ADR: IPSEY), today announced that Somatuline® ... Somatuline®) was approved by the U.S. Food and ... neuroendocrine tumors (GEP-NETs) in adult patients with unresectable, ... disease to improve progression-free survival (PFS). , “The ...
Breaking Biology Technology:iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 2Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 8
... are being held to solicit public sentiment on Assembly ... 45 . The bills were introduced to the state legislature ... and parthenogenesis (the process of manipulating the genetic material of ... material from any other cell, in a way that causes ...
... just go bad. Not so often, components that supply power ... case beginning at 11:57 p.m. Monday night with TDS Internet ... and downed dial-up and DSL users en masse, TDS Telecom ... restored at 2:40 a.m. to all users. , ,TDS Internet ...
... determined to give heavy email users something they lack: time and ... a heavy email user, talks about the inspiration for his company, ... world. , , WTN: How much email do you get everyday? ... messages a day from various email accounts with about 70% of ...
Cached Biology Technology:Public Hearing Held for controversial anti-cloning bills 2Public Hearing Held for controversial anti-cloning bills 3Public Hearing Held for controversial anti-cloning bills 4TDS Suffers 29-State System Outage 2TDS Suffers 29-State System Outage 3WTN Interview: Alex Bratton: EmailRX's founder on viruses and spam 2WTN Interview: Alex Bratton: EmailRX's founder on viruses and spam 3
(Date:12/10/2014)... RALEIGH, N.C. , Dec. 9, 2014  Valencell, ... announced it is seeing a staggering demand from its ... demand, the Company revealed, is not solely coming from ... industries as well. "A wearable is only ... a result, accuracy is the ultimate driver in long-term ...
(Date:12/10/2014)... Dec. 9, 2014 CIE San Diego has ... provides the connective tissue that enhances care coordination among ... easily share client-level information; earned a second $1 million ... organizations serving seniors aging in community and; will be ... December 11 th 4-6p. CIE San ...
(Date:12/10/2014)... 2014     ...   Jifflenow, a leading ... for business-to-business (B2B) events, today announced a partnership ... near-field communication (NFC), Bluetooth low energy (BLE), and ... , Jifflenow will integrate its meeting ...
Breaking Biology News(10 mins):Wearable Technology Products Demand Highly Accurate Biometric Technology 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3
... BOULDER, Colo., September 11, 2012 BiOptix announced continued support ... in BioWest 2012, a leading life sciences showcase ... City Center. "BiOptix continues to believe in the ... Association, noted Rick Whitcomb, President and CEO of ...
... screening technique developed by researchers at the RIKEN Plant ... novel immune-priming compounds in Arabidopsis plants. Discovery ... plant growth or crop yield, establishes the new technique ... crops from damaging pathogens. Plant activators, compounds that ...
... Mich.A strip of glass covered in hairy nanoparticles can ... system, and other toxic metals in fluids. ... Northwestern University and the University of Michigan found that ... common form of mercury pollution, at unprecedentedly small concentrations. ...
Cached Biology News:BiOptix Participates in Leading Life Sciences Event BioWest 2012 2Screening technique uncovers 5 new plant activator compounds 2Measuring mercury levels: Nano-velcro detects water-borne toxic metals 2Measuring mercury levels: Nano-velcro detects water-borne toxic metals 3
Rabbit polyclonal to KIAA1276 ( Abpromise for all tested applications). entrezGeneID: 27146 SwissProtID: Q9ULE4...
Rabbit polyclonal to IL23 P19 ( Abpromise for all tested applications). Antigen: Synthetic 16 amino acid peptide from near the amino terminus of human IL23 P19 Entrez Gene ID: 51561 Swiss...
Monoclonal Mouse Anti-Human Cytokeratin 16 (48 kD)...
PFM8 Antibody...
Biology Products: