Navigation Links
TransMessenger Transfection Reagent Handbook

ng a particular cell type/RNA combination.

Cell density at transfection
Table 1 lists the recommended number of adherent cells to seed per culture well the day prior to transfection of single-stranded RNA. The optimal confluency for transfection of adherent cells with single-stranded RNA is 8090%, while the optimal confluency for transfection of adherent cells with siRNA is 5080%. The optimal confluency should be determined for every new cell type to be transfected and kept constant in future experiments. This is achieved by counting cells prior to seeding and by keeping the time period between seeding and transfection (minimum 24 hours) constant. This will ensure that the cell density is not
too high and that the cells are in optimal physiological condition on the day of transfection.

Table 1. Recommended number of cells to seed the day before transfection of single-stranded RNA * Be sure that cells are seeded a minimum of 24 hours before transfection of RNA. Actual cell number used depends on cell type and size. Sufficient cells should be seeded such that the culture is 8090% confluent on the day of transfection. The volume of medium used is not critical. Use a volume suitable for the cell culture format. Amount of RNA
The amount of RNA used is a critical factor for efficient transfection. Toxic effects may arise if too much RNA is used, while conversely, expression levels may be too low if insufficient RNA is used. Therefore, the amount of RNA transfected should be optimized for every new RNA and/or new cell type used. The recommended amount of RNA for transfection using 6-well plates i
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:4/24/2015)... April 24, 2015 Seoul Semiconductor ... for various applications in the lighting market. ... installations of this technology in emerging markets such ... a wide range of applications such as streetlight ... decorative lighting. , First launched in 2005 the ...
(Date:4/23/2015)... , April 23, 2015 /CNW/ - A new and ... award from North America,s ... Comparison won Silver in the "Sustainability, service" category ... The Brandcheck "Competitor Comparison" is a service that ... American and Global peers. This benchmarking process shows ...
(Date:4/23/2015)... SHANGHAI , April 23, 2015 WuXi ... open-access R&D capability and technology platform company serving the ... China and the ... release financial results for the first quarter of 2015 ... May 13, 2015 (which will be Thursday morning, May ...
(Date:4/23/2015)... Prospects for Leading Companies in OTC, Rx Pharmaceuticals ... We predict the global healthcare packaging market ... will grow at a CAGR of 6.3% between 2015 and ... and increasing functional requirements for healthcare packaging products are some ... packaging market. A global ageing population, changing lifestyles and ...
Breaking Biology Technology:Acrich Technology from Seoul Semiconductor Adopted in Installations Worldwide 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 3Healthcare Packaging Market Forecast 2015-2025 2Healthcare Packaging Market Forecast 2015-2025 3Healthcare Packaging Market Forecast 2015-2025 4Healthcare Packaging Market Forecast 2015-2025 5Healthcare Packaging Market Forecast 2015-2025 6Healthcare Packaging Market Forecast 2015-2025 7Healthcare Packaging Market Forecast 2015-2025 8Healthcare Packaging Market Forecast 2015-2025 9Healthcare Packaging Market Forecast 2015-2025 10
... IgG1 Using UNOsphere S and CHT , ... , Henry Lai, PhD, and Samuel G Franklin, PhD, ... USA , , ... a variety of sources have been purified , using ...
... Agencourt offers primer walking sequencing of PCR products, ... submit your template in either raw or , ... Select from either single stranded coverage for confirmation sequencing , ... clone validation , purposes. , ...
... , RNAClean is a ... isolates RNA or cDNA from in vitro transcription, cDNA or RNA ... As gene expression profiling , analysis and ... effective, automated purification reagent is required. Traditional purification , ...
Cached Biology Technology:Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 2Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 3Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 4Primer Walking 2RNA & cDNA Purification Using RNAClean 2RNA & cDNA Purification Using RNAClean 3RNA & cDNA Purification Using RNAClean 4RNA & cDNA Purification Using RNAClean 5
(Date:3/19/2015)... March 19, 2015  NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, announces its biometric payment ... clip that aired this week on Washington DC,s ... In a segment "The Next Great Thing", host ... way to pay, and ,a really big breakthrough in mobile ...
(Date:3/17/2015)... , March 17, 2015 ... company specializing in clinical study management systems, is ... in attendance at ARENA,s Outsourcing in Clinical Trials ... This event provides an excellent forum for MedNet ... flexible and affordable software-as-a-service (SaaS) eClinical technology platform. ...
(Date:3/17/2015)... YORK , March 17, 2015  MecklerMedia ... the largest exhibition for service robots ever held ... RoboUniverse takes place on May 11-13, 2015 at ... and exhibitors for the event include: Acorn Product ... Control; EZ-Robot; Infranor; Kinova Robotics; Littler; NewBotic Corporation; ...
Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 2NXT-ID's Wocket Smart Wallet Featured on Fox News Segment, "The Next Great Thing" 3Connect With MedNet Solutions At Outsourcing In Clinical Trials Southeast 2MecklerMedia's RoboUniverse New York Announces Sponsors and Exhibitors, May 11-13, 2015 2
... hearing loss is the most common sensory disorder among the ... cellular processes govern or contribute to the loss. Now ... Wisconsin and three other institutions have identified a protein that ... and lead to age-related hearing loss. The findings help ...
... MADISON, WI, NOVEMBER 9, 2009 -- Agricultural ... a result of their presence in wastes (sewage biosolids ... periods of time. Regulations and guidelines for tolerable concentrations ... on the assumption that the toxic effects of the ...
... Sciences today announced it will give its highest science ... more than anyone to document one of the most ... the world,s authority on grasshoppers and a world authority ... for scientific excellence on Thursday, Nov. 12, at the ...
Cached Biology News:'Escaped' proteins add to hearing loss in elderly, UF researchers find 2'Escaped' proteins add to hearing loss in elderly, UF researchers find 3Additive copper-zinc interaction affects toxic response in soybean 2Noted entomologist to receive science excellence award 2Noted entomologist to receive science excellence award 3
... oligodeoxynucleotides are random primers ... the 5' end with ... dyes and they are ... spot morphology, hybridization potential ...
EDTA, disodium salt, 1 kg. Ethylene diaminetetraacetic acid.Dihydrate. Assay: 99.0-101.0%, nuclease-free.Risks: 36, 37, 38 ; Safety Precautions: 22, 36, 37. Category: Nucleotides & Enzymes & Biochemi...
Mycoplasma tested...
Alkaline Phosphatase Stabilizer Solution, 1 L...
Biology Products: