Navigation Links
TransMessenger Transfection Reagent Handbook

ng a particular cell type/RNA combination.

Cell density at transfection
Table 1 lists the recommended number of adherent cells to seed per culture well the day prior to transfection of single-stranded RNA. The optimal confluency for transfection of adherent cells with single-stranded RNA is 8090%, while the optimal confluency for transfection of adherent cells with siRNA is 5080%. The optimal confluency should be determined for every new cell type to be transfected and kept constant in future experiments. This is achieved by counting cells prior to seeding and by keeping the time period between seeding and transfection (minimum 24 hours) constant. This will ensure that the cell density is not
too high and that the cells are in optimal physiological condition on the day of transfection.

Table 1. Recommended number of cells to seed the day before transfection of single-stranded RNA * Be sure that cells are seeded a minimum of 24 hours before transfection of RNA. Actual cell number used depends on cell type and size. Sufficient cells should be seeded such that the culture is 8090% confluent on the day of transfection. The volume of medium used is not critical. Use a volume suitable for the cell culture format. Amount of RNA
The amount of RNA used is a critical factor for efficient transfection. Toxic effects may arise if too much RNA is used, while conversely, expression levels may be too low if insufficient RNA is used. Therefore, the amount of RNA transfected should be optimized for every new RNA and/or new cell type used. The recommended amount of RNA for transfection using 6-well plates i
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:3/3/2015)... 2015 HubCast® Inc. ( http://www.hubcast.com ), ... the expansion of its Global Print Supply Chain with ... the world’s largest cities across 75 countries globally. ... countries providing global enterprises ease, speed, reach, quality, cost ... online order to real-world delivery at postal address around ...
(Date:3/3/2015)... March 3, 2015 Kindred Biosciences, Inc. (NASDAQ: ... improving the lives of pets, announced today that it will ... March 12, 2015 after the market close. The Company will ... day. Interested parties may access the call ... 756-4262 internationally, and using conference ID 98449349.  ...
(Date:3/3/2015)... DUBLIN , Mar. 03, 2015 Research and ... addition of Jain PharmaBiotech,s new report "RNAi - ... , Because of its ability to silence ... been adopted as the research tool to discriminate gene ... RNAi can be designed to target every gene in ...
(Date:3/3/2015)... , March 3, 2015  Synthetic Biologics, Inc. (NYSE ... and diseases, with a focus on protecting the microbiome, announced ... scheduled to present at the 27th Annual ROTH Conference being ... Laguna Niguel in Dana Point, CA. ... March 10, 2015 at 1:00 p.m. (Pacific Time). ...
Breaking Biology Technology:HubCast Expands Global Print Supply Chain: Launches New Product Suite 2HubCast Expands Global Print Supply Chain: Launches New Product Suite 3HubCast Expands Global Print Supply Chain: Launches New Product Suite 4Global RNAi Market Report 2015 - Technologies, Markets and Companies 2014-2024 2Global RNAi Market Report 2015 - Technologies, Markets and Companies 2014-2024 3Synthetic Biologics to Present at the 27th Annual ROTH Conference 2
... repairing damaged human tissue doesn,t wrinkle up when it ... of California, San Diego marks a significant breakthrough in ... of native human tissue. Shaochen Chen, professor ... Diego Jacobs School of Engineering, hopes future tissue patches, ...
... Mass., May 26, 2011 New England Biolabs (NEB), ... reagents for the life science industry, extends its global ... France, located at Bioparc Genopole in Evry, just south ... Ellard, President and CEO of NEB Inc., was on ...
... 2011 Addressing the issue of data overload from next-generation ... ) today launched its LifeScope™ 2.0 Genomic Analysis Software ... genomic sequencing instruments to study human disease such as cancer. ... A single next generation sequencing instrument can generate nearly 200 ...
Cached Biology Technology:Nanoengineers invent new biomaterial that more closely mimics human tissue 2New England Biolabs Opens New Subsidiary Office in France 2Life Technologies Simplifies, Organizes and Speeds Analysis of Genomic Data Through Launch of New LifeScope Bioinformatics Software 2Life Technologies Simplifies, Organizes and Speeds Analysis of Genomic Data Through Launch of New LifeScope Bioinformatics Software 3Life Technologies Simplifies, Organizes and Speeds Analysis of Genomic Data Through Launch of New LifeScope Bioinformatics Software 4Life Technologies Simplifies, Organizes and Speeds Analysis of Genomic Data Through Launch of New LifeScope Bioinformatics Software 5
(Date:2/5/2015)... Jan. 26, 2015 NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile commerce ... ( http://www.nxt-id.com/ ) as part of its 2015 marketing ... Company launched its new consumer website for Wocketwallet.com earlier ... CEO of NXT-ID said, "Our new corporate website naturally ...
(Date:2/5/2015)... 2015 New Market Research Reports ... Forecasts To 2020 has Been Added to GrandViewReseach.com Report ... expected to reach USD 5.10 billion by 2020, according ... IR cameras help identify the site of tissue damage ... surging demand in medical imaging applications. They have been ...
(Date:2/5/2015)... , Jan. 28, 2015 Research and ... the addition of the "Global Biometrics Market ... Regions and Countries " report to their offering. ... Asia-Pacific is anticipated to ... 2015, owing to increasing government spending towards IT ...
Breaking Biology News(10 mins):NXT-ID, Inc. Launches New Corporate Website As Part Of Ongoing 2015 Branding Initiatives For Wocket Smart Wallet 2NXT-ID, Inc. Launches New Corporate Website As Part Of Ongoing 2015 Branding Initiatives For Wocket Smart Wallet 3IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 2Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 3
... used to eradicate weeds. But a study published today ... chemical may be eradicating much more than that. , ... Roundup®, the second most commonly applied herbicide in the ... experiment is one of the most extensive studies on ...
... is the most lifeless spot on the face of the Earth, ... of NASA scientists there earlier this month. , The University of ... they'd built could detect life in that crusty, arid land, then ... on the planet Mars. , In a place that hadn't seen ...
... to select and develop compounds that act on specific ... mathematical algorithm that predicts the precise effects a given ... chemical processes. Using this tool, drug developers can design ... protein targets, eliciting therapeutic responses free of unwanted side ...
Cached Biology News:Roundup®highly lethal to amphibians, finds University of Pittsburgh researcher 2Life detection instrument passes key test on road to Mars 2Life detection instrument passes key test on road to Mars 3Computational Tool Predicts How Drugs Work In Cells, Advancing Efforts To Design Better Medicines 2