Navigation Links
TransMessenger Transfection Reagent Handbook

followed throughout the procedure.

General handling
Proper microbiological, aseptic technique should always be used when working with RNA. Hands and dust particles may carry bacteria and molds, and are the most common sources of RNase contamination. Always wear latex or vinyl gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin. Change gloves frequently and keep tubes closed. Ensure that all glassware, plasticware, and solutions (including water and culture medium) are RNase-free.

Disposable plasticware
The use of sterile, disposable, plastic tubes throughout the procedure is recommended. These tubes are generally RNase-free and do not require pretreatment to inactivate RNases.

DNA contamination
No currently available purification method can guarantee that RNA is completely free of DNA, even when no DNA is visible on an agarose gel. For RNA transfection, digestion of the purified RNA with RNase-free DNase is recommended. The RNA can then be repurified using the RNeasy Kit.

Optimizing RNA Transfection

To achieve optimal transfection efficiency for every cell typeRNA combination, we recommend optimizing a number of parameters:

The amounts of TransMessenger Transfection Reagent, RNA, and TransMessengerRNA complexes
The cell number/confluency
The length of exposure of cells to TransMessengerRNA complexes

Once the parameters yielding maximum transfection efficiency have been determined, they should be kept constant in every experiment usi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/15/2014)... The Pittcon Organizing Committee is pleased to announce that ... an e-Journal and producer of Food Labs Conference ... for the co-location of Food Labs Conference to be held ... registration fee to attend the two-day Food Lab Conference, March ...
(Date:1/15/2014)... England and SAN JOSE, California ... international biotechnology company developing antibody-drug conjugates for cancer, today announced ... Board of Directors. Dr Reynolds has over 20 years, development ... Medical Officer at Seattle Genetics. "I am delighted ...
(Date:1/15/2014)... Houston, TX (PRWEB) January 15, 2014 ... Alzheimer’s disease, and about 1 in 3 seniors will ... ( source ). These jaw-dropping figures have shocked many ... function and, hopefully, help prevent these tragic age-related cognitive ...
(Date:1/15/2014)... January 15, 2014 2013 was a ... Brain State Technologies®. They saw continued independent research led ... who were awarded a $1 million grant from the ... “Brain and Behavior” a peer reviewed journal, Amy Grant ...
Breaking Biology Technology:Pittcon Announces Second Year Partnership With Food Safety Tech 2Pittcon Announces Second Year Partnership With Food Safety Tech 3Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3
... For a successful bioscience company there are many moving ... knowledgeable workforce to suppliers and market access, each of ... today,s modern scientific challenges. Yet, leveraging these solutions in ... R&D are high. Complexity in the supply chain can ...
... 2011 NeoStem, Inc. ("NeoStem" or the "Company") (NYSE ... with product and service revenues, global research and development ... U.S. adult stem cells, China adult stem cells, and ... today announced steps forward in their partnership designed to ...
... June 16, 2011 /PRNewswire-Asia/ -- WuXi AppTec announced today that ... Outsourcing Enterprises in China for 2011 by China,s Ministry of ... Service Outsourcing Cooperation Conference, held in Nanjing on June 10, ... WuXi AppTec, was named a Top Ten Contributor to China,s ...
Cached Biology Technology:Ohio's Bioscience Industry Thrives With World-Class Industry Collaboration 2Ohio's Bioscience Industry Thrives With World-Class Industry Collaboration 3Ohio's Bioscience Industry Thrives With World-Class Industry Collaboration 4The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 2The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 3The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 4The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 5The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 6The Pontifical Council for Culture and NeoStem Announce Steps Forward in their Partnership to Advance Adult Stem Cell Research 7
(Date:4/17/2014)... Australian National University (ANU), have developed a new ... variability over the past 5.3 million years., The ... surrounding ice ages over the past two million ... carbon dioxide levels, global temperatures and sea levels., ... (UoS) and the National Oceanography Centre (NOC) in ...
(Date:4/17/2014)... of our ideas about domestication derive from Charles ... by British animal-breeding practices during the 19th century, ... improvement. , It is from Darwin that ... of captive animals from wild species and total ... But animal management in this industrial setting has ...
(Date:4/16/2014)... in museum collections two new species of yellow-shouldered bats have been ... The Field Museum of Natural History and described in the open ... to the genus Sturnira are part of a recent ... the third one still waiting to be officially announced. , Up ...
Breaking Biology News(10 mins):Ancient sea-levels give new clues on ice ages 2The story of animal domestication retold 2The story of animal domestication retold 3The story of animal domestication retold 4Two new species of yellow-shouldered bats endemic to the Neotropics 2
... Scientists and engineers from around the world will convene ... discuss recent advances in optics and photonics -- the ... -- affecting renewable energy and environmental research. Journalists ... Photonics Congress: Renewable Energy & the Environment, which will ...
... rats, European researchers have proved that eating strawberries reduces ... mucous membrane. Published in the open access journal Plos ... of stomach ulcers. A team of Italian, Serbian ... strawberries have in a mammal stomach that has been ...
... brain protein that regulates gene expression may play a role ... disabling psychiatric disease. As reported in the latest issue of ... for Bipolar Disorders, levels of SP4 (specificity protein 4) were ... samples from patients with bipolar disorder. The study suggests that ...
Cached Biology News:Space debris, more efficient LEDs, and thinner, cheaper solar cells 2Space debris, more efficient LEDs, and thinner, cheaper solar cells 3Space debris, more efficient LEDs, and thinner, cheaper solar cells 4Space debris, more efficient LEDs, and thinner, cheaper solar cells 5Strawberries protect the stomach from alcohol 2Gene regulatory protein is reduced in bipolar disorder 2Gene regulatory protein is reduced in bipolar disorder 3
...
Quinolinate phosphoribosyltransferase...
This antibody is specific for human J-chain as defined by ELISA assay using purified human immunoglobulin fragments as solid phase target antigen....
Tissue-Stat ( Tissue Adherent for Mounting Formalin-Fixed and Frozen Tissue Sections.)...
Biology Products: