Navigation Links
TransMessenger Transfection Reagent Handbook

followed throughout the procedure.

General handling
Proper microbiological, aseptic technique should always be used when working with RNA. Hands and dust particles may carry bacteria and molds, and are the most common sources of RNase contamination. Always wear latex or vinyl gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin. Change gloves frequently and keep tubes closed. Ensure that all glassware, plasticware, and solutions (including water and culture medium) are RNase-free.

Disposable plasticware
The use of sterile, disposable, plastic tubes throughout the procedure is recommended. These tubes are generally RNase-free and do not require pretreatment to inactivate RNases.

DNA contamination
No currently available purification method can guarantee that RNA is completely free of DNA, even when no DNA is visible on an agarose gel. For RNA transfection, digestion of the purified RNA with RNase-free DNase is recommended. The RNA can then be repurified using the RNeasy Kit.

Optimizing RNA Transfection

To achieve optimal transfection efficiency for every cell typeRNA combination, we recommend optimizing a number of parameters:

The amounts of TransMessenger Transfection Reagent, RNA, and TransMessengerRNA complexes
The cell number/confluency
The length of exposure of cells to TransMessengerRNA complexes

Once the parameters yielding maximum transfection efficiency have been determined, they should be kept constant in every experiment usi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/22/2015)... 22, 2015 Dr. Greg Leyer of UAS ... Scripps Natural Supplements Pre-Conference seminar on probiotics in San Diego, ... annual continuing education conference for health care professionals. This year’s ... topic of probiotics in health. Dr. Leyer spoke about the ...
(Date:1/22/2015)... Diagenode, Inc., a leading global ... and complete solutions for epigenetics research, recently launched ... the need for manual processing. The new ChIPettor ... of histones or transcription factors and a semi-automated ...
(Date:1/22/2015)... 22. 2015  Varian Medical Systems (NYSE: VAR ), ... for its commitment to sustainability with inclusion on a prestigious ... highest ranked healthcare equipment company among the Corporate Knights Global ... Economic Forum at Davos, Switzerland . ...
(Date:12/25/2014)... report “Service Quality Management and Telco Customer Experience Management ... Advancements, Worldwide Forecasts & Analysis (2014-2019)” defines and segments ... revenues. , Browse 152 market data tables, 19 ... “Service Quality Management (SQM) and Telco Customer Experience Management ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Varian Honored Among World's 100 Most Sustainable Corporations 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... Plasma Protein Therapeutics Association (PPTA) recommends that the Centers ... to improve drug reimbursement under the Hospital Outpatient Perspective ... Panel on Ambulatory Payment Classification (APC) Groups meeting, PPTA ... hospitals that purchase drugs under the 340B program from ...
... 2011 Reportlinker.com announces that ... is available in its catalogue: ... Diagnostic Technologies: Assessment of Current ... Potential Market Applications ...
... 18, 2011 Creabilis SA, a clinical ... dermatology, inflammation and pain, announced today the expansion of ... experienced international pharma executives. Alex Leech joins as Vice ... Senior Vice President, Head of Development, and Peter Spargo ...
Cached Biology Technology:PPTA Urges CMS for Appropriate Hospital Outpatient Reimbursement for Plasma Protein Therapies 2PPTA Urges CMS for Appropriate Hospital Outpatient Reimbursement for Plasma Protein Therapies 3PPTA Urges CMS for Appropriate Hospital Outpatient Reimbursement for Plasma Protein Therapies 4Tumor Marker Diagnostic Technologies: Assessment of Current and Emerging Technologies, And Their Potential Market Applications 2Creabilis Expands Senior Management Team 2Creabilis Expands Senior Management Team 3
(Date:12/22/2014)... Holiday Season may be the brightest ever for the ... long anticipated floodgates for consumer biometrics may finally be ... tablets, and wearable mobile devices that incorporate biometrics will ... nearly 4.8 billion biometric devices by 2020.   ...
(Date:12/19/2014)... , Dec. 18, 2014   LaunchKey , the first ... post-password and Internet of Things era, today announced the ... venture round was led by Metamorphic Ventures with participation ... Partners, VegasTechFund, and others.  LaunchKey has raised $4 million ...
(Date:12/17/2014)... Valencell, a leader in performance biometric data sensor ... leaders such as Intel, Jabra, Atlas, and Scosche to ... These products will be showcased at CES 2015, taking ... . "Our partners choose PerformTek biometrics, ...
Breaking Biology News(10 mins):First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2
... tactile learning book, "Touch the Earth," on Monday, April 19, ... on the National Mall organized by the Earth Day Network. ... published last month, with its authors and illustrators on hand ... a multimedia approach to teach middle school students about the ...
... climate change, a new book by Claire L. Parkinson, ... Center in Greenbelt, Md., can provide some answers. ... Beware the Big Fix," will be published this week ... warming is such a big deal, summarizes 4.6 billion ...
... the Carnegie Institution,s Department of Global Ecology, is among ... the humanities and the arts, business, public affairs, and ... new class of members of the American Academy of ... most prestigious honorary societies, cited Field for his research ...
Cached Biology News:'Touch the Earth' to display at Earth Day on National Mall 2NASA scientist publishes new book: 'Coming Climate Crisis? Consider the Past, Beware the Big Fix' 2Carnegie's Chris Field elected to American Academy of Arts & Sciences 2
... introduced QPatch HT is taking automated patch clamping ... With its ability to generate thousands of high ... new screening paradigms in ion channel drug discovery ... same high quality afforded by conventional patch clamping ...
... aptamer screening and binding optimization and built ... on-chip synthesis platform. These microarrays are available ... Microarray Service. Probe Content ... greater than 1500 known aptamer sequences. Add ...
... provides a comprehensive service for custom ... peptide microarrays synthesized on Paraflo microfluidic ... proteins/antibodies or serum samples. This comprehensive ... designs, binding assays using sample(s) provided ...
... aptamer screening and binding optimization and built ... on-chip synthesis platform. Thousands of customer specified ... Sciences can provide assistance with custom sequence ... of our comprehensive DNA/RNA Aptamer Microarray Service. ...
Biology Products: