Navigation Links
TransMessenger Transfection Reagent Handbook

Cells have been
passaged too
many times Cells that have been passaged a large number of times tend to change their growth behavior, morphology, and transfectability. When cells with high passage numbers are used for replicate experiments, decreased transfection efficiencies may be observed in later experiments. We recommend using cells with a low passage number (<50 splitting cycles). No or very small
gene silencing
effect observed
after siRNA
transfection
Design of siRNA
sub-optimal The design of an siRNA can have a large effect on its gene silencing efficiency. QIAGEN offers expert advice on siRNA design for maximum gene silencing effect. For more information visit www.qiagen.com/sirna. Incubation time
post-transfection too
short The gene silencing effect observed on the protein level is dependent on a proteins expression level and its rate of turnover within the cell. Perform a time course experiment to determine the optimal time point for analysis. Problems with
experimental design RNAi effects may not be seen for some genes targeted with certain siRNAs in some cell types. If possible, repeat experiments using a different cell type and/or siRNA. Include where possible both positive and negative controls in your experiments. QIAGEN offers siRNA that has been functionally tested for s
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:7/23/2014)... 2014 DuPont Pioneer announced today that ... president of Agricultural Biotechnology (ABT), effective July 21, 2014. ... most recently as president, chief executive officer and board ... in the seed and crop protection industry to this ... Schickler , president of DuPont Pioneer. “I am looking ...
(Date:7/23/2014)... BRUSSELS , July 23, 2014 regulated ... its research and development pipeline with positive topline ... brivaracetam . This study was designed to evaluate ... (100 and 200 mg/day, without titration) compared to ... patients with partial-onset seizures, not fully controlled despite ...
(Date:7/23/2014)... 23, 2014  GrowBLOX Sciences, Inc. (OTCQB: GBLX), a medical ... filing for two medical marijuana dispensary locations in ... to the Clark County, Nevada dispensary ... as previously announced.  GrowBLOX Sciences, CEO Craig ... new applications for dispensaries in the City of ...
(Date:7/23/2014)... China Cord Blood Corporation (NYSE: CO ) ("CCBC" or ... blood collection, laboratory testing, hematopoietic stem cell processing, and stem cell ... catering to the mother and child segment, today announced that the ... Singapore , Hong Kong , ... the Philippines and Malaysia ...
Breaking Biology Technology:DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 3Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 2Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 3Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 4Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 5Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 6Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 7GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 2GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 3China Cord Blood Corporation and Cordlife Group Limited Announce Collaboration to Aid Asia-based Patients with Cord Blood Stem Cell Therapy 2China Cord Blood Corporation and Cordlife Group Limited Announce Collaboration to Aid Asia-based Patients with Cord Blood Stem Cell Therapy 3China Cord Blood Corporation and Cordlife Group Limited Announce Collaboration to Aid Asia-based Patients with Cord Blood Stem Cell Therapy 4
... Partners announced,today that it has initiated coverage on ... promising drug development pipeline for treating a,variety of ... on two,drug development programs; the first is MN-221 ... estimated 2,000,000 visits to the emergency room,a year ...
... Third Party Administrators,Alliance (TPAA) announced today the appointment ... the nation,s first alliance of third party,administrators and ... to its members., Oelschlager brings hands-on experience ... role, assets he believes will further the,organization,s reach ...
... PRXL ), a leading global biopharmaceutical services,organization, ... Goldberg, M.D.,to Chief Operating Officer (COO). Dr. Goldberg ... Perceptive Informatics at,PAREXEL. He will continue to oversee ... von Rickenbach, Chairman and Chief Executive Officer of ...
Cached Biology Technology:Vista Partners Initiates Coverage on MediciNova Inc. (Nasdaq: MNOV) 2The TPAA Names Mark Oelschlager President 2PAREXEL Promotes Dr. Mark A. Goldberg to Chief Operating Officer 2PAREXEL Promotes Dr. Mark A. Goldberg to Chief Operating Officer 3PAREXEL Promotes Dr. Mark A. Goldberg to Chief Operating Officer 4
(Date:7/24/2014)... into the potato genes that best adapt to the ... rainfall and increased extremes of hot and cold temperatures.The ... order to create new potato varieties that will adapt ... to find out how the current potato varieties will ... and lower temperatures. , This research is part of ...
(Date:7/24/2014)... primordial soup in which life arose have been maintained in ... East Anglia. , Research published today in the Journal ... and very likely also in animals still perform ancient reactions ... some four billion years ago. , The primordial soup ... as a result of the combination of metals, gases from ...
(Date:7/24/2014)... led by the University of York has carried out ... Western Indian Ocean. , The results, reported in ... revolution in the management of marine protected areas, with ... km in the region now under local community ... the seas and coasts designed to protect wildlife from ...
Breaking Biology News(10 mins):Neiker-Tecnalia is researching the potato genes that best adapt to climate change 2Western Indian Ocean communities play vital role in conservation 2
... revealed a previously unknown mechanism in the body which ... responses and control of blood pressure, pain and appetite. ... help fight health problems connected with these functions in ... and University College London found that lactate essentially ...
... New York, NYFebruary 10, 2014In a study published ... team led by Ken Shepard, professor of electrical ... Lars Dietrich, assistant professor of biological sciences at ... the basis of modern computers and communications devices, ...
... change unfolds over the next century, plants and animals will ... climate. A new study provides an innovative global map of ... up with a changing climate. The findings appear in the ... a UC Santa Barbara National Center for Ecological Analysis and ...
Cached Biology News:New research sheds light on how the body regulates fundamental neuro-hormone 2Chips that listen to bacteria 2Maps show expected redistribution of global species due to climate change 2Maps show expected redistribution of global species due to climate change 3
... MasterAmp™ Taq DNA Polymerase is a ... aquaticus. Having optimal activity at temperatures ... thermostable enzyme used for PCR and ... purpose. The enzyme has an intrinsic ...
... 4100 high pressure dual piston pump and the ... and utra low drift. The WaveQuest ... detection and provides superior stability. Hence ... and for monitoring to the highest of sensitivities. ...
Rabbit anti Xanthine Oxidase Xanthine Oxidase, Bovine Buttermilk...
... This CLS number ... number, created to easily ... If showing no availability ... the old Sigma-Aldrich number ...
Biology Products: