Navigation Links
TransMessenger Transfection Reagent Handbook

Cells have been
passaged too
many times Cells that have been passaged a large number of times tend to change their growth behavior, morphology, and transfectability. When cells with high passage numbers are used for replicate experiments, decreased transfection efficiencies may be observed in later experiments. We recommend using cells with a low passage number (<50 splitting cycles). No or very small
gene silencing
effect observed
after siRNA
transfection
Design of siRNA
sub-optimal The design of an siRNA can have a large effect on its gene silencing efficiency. QIAGEN offers expert advice on siRNA design for maximum gene silencing effect. For more information visit www.qiagen.com/sirna. Incubation time
post-transfection too
short The gene silencing effect observed on the protein level is dependent on a proteins expression level and its rate of turnover within the cell. Perform a time course experiment to determine the optimal time point for analysis. Problems with
experimental design RNAi effects may not be seen for some genes targeted with certain siRNAs in some cell types. If possible, repeat experiments using a different cell type and/or siRNA. Include where possible both positive and negative controls in your experiments. QIAGEN offers siRNA that has been functionally tested for s
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:8/28/2014)... (PRWEB) August 28, 2014 SPIE Fellow ... Physics and Optical Science and Director of the Center ... North Carolina at Charlotte, and co-founder and Chairman of ... serve as the 2015 Vice President of SPIE, the ... President Philip Stahl announced recent election results at the ...
(Date:8/28/2014)... are important devices in spintronics, an electronic which ... electrons but also on their spin and the ... electric into magnetic signals and vice versa. Recently, ... the Institute of Physics at Johannes Gutenberg University ... Prague, and Japan, has for the first time ...
(Date:8/28/2014)... 2014 Sterlitech is proud to announce ... its stable of products . These additional ... and include membrane filters with surface charges. ... demand for our membrane process testing equipment, and correspondingly, ... sheet membranes,” explains Sterlitech President Mark Spatz. “The ...
(Date:8/28/2014)... this month, a report was created by Colorado ... Force on the performance of vehicles using an eco-friendly, ... fuel made from mustard seeds, offered great potential as ... friendly future. In fact, every branch of the U.S. ... foreign oil over the next few years. They will ...
Breaking Biology Technology:Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2A new, tunable device for spintronics 2A new, tunable device for spintronics 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 2Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 3Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 4Can Small Farmers and a New Seed Based Biofuel “Green” the World? Major Universities and Even the U.S. Air Force Seem to Think So 5
... U.S. Asthma Control, RESEARCH TRIANGLE PARK, N.C. and ... with asthma and more than a quarter of,children with ... have their disease well controlled, according to the Asthma ... of the American,Thoracic Society meeting in Toronto., The ...
... May 20 PreViser Corporation,( http://www.previser.com ), a ... assessment technology to the dental profession,announced today the ... (OHI) Ltd ( http://www.previser.co.uk ) of Birmingham, England ... Kingdom and the,Republic of Ireland., OHI Ltd ...
... living computers by genetically altering bacteria. The findings of ... of Biological Engineering, demonstrate that computing in living cells ... applications including data storage and as a tool for ... from the biology and the mathematics departments of Davidson ...
Cached Biology Technology:Asthma Uncontrolled in More Than 40 Percent of Adults With the Disease, New Landmark Survey Shows 2Asthma Uncontrolled in More Than 40 Percent of Adults With the Disease, New Landmark Survey Shows 3PreViser and Oral Health Innovations Ltd Form UK Partnership to Provide Oral Health Scoring and Risk Analysis 2Researchers bring new meaning to the term 'computer bug' 2
(Date:8/29/2014)... University of Texas Medical Branch at Galveston has gone on ... completely protect monkeys against a lethal dose of Ebola virus ... the disease is severe. , Thomas Geisbert, professor of microbiology ... discussing advances in Ebola treatment research. The filoviruses known as ... of pathogens, with fatality rates of up to 90 percent. ...
(Date:8/29/2014)... connections in transcription factor networks (TFNs) to evaluate the ... in PLOS Computational Biology in August. ... a network,s resilience against mutations. , "In simulations, ... models has a greater effect on robustness than varying ... Dov A. Pechenick, PhD, lead author and former researcher ...
(Date:8/29/2014)... This news release is available in German . ... of chemical signals between organisms is considered the oldest ... social interactions between conspecifics, for example, the sexual attraction ... trigger social responses and to coordinate reproductive behavior in ... at the University of the Algarve in Faro, Portugal, ...
Breaking Biology News(10 mins):Leading Ebola researcher at UTMB says there's an effective treatment for Ebola 2Assortativity signatures of transcription factor networks contribute to robustness 2Ready for mating at the right time 2Ready for mating at the right time 3
... The most common cause of a mare,s reduced fertility is ... caused by the bacteria , Streptococcus equi subspecies ... negative for streptococci have a dormant infection without showing clinical ... to reduce the fertility of the mares," says Professor Anders ...
... Researchers at the Johns Hopkins University in Baltimore, Md., ... have found that nonself-donated cells (allografts) better survive implantation ... grafts are injected into the striatum (STR) of the ... region. In their study, all FM grafts were ...
... being passive wallflowers, are "secret gardeners" that actively engineer their ... team of scientists from Duke University and the University of ... that the distribution of plants within a marsh is a ... that,s where conditions like soil aeration and salinity best meet ...
Cached Biology News:Researchers make racehorses fertile 2Impact of stem cell transplantation location in brain a crucial factor for cell survival 2Marsh plants actively engineer their landscape 2
Goat polyclonal to MLH3 Immunogen: Synthetic peptide: RQSLQQSMPPCEPP (Human) from C Terminus of the protein sequence according to NP_055196....
Polyclonal Antibody to SHPRH...
Rabbit polyclonal to TetR ( Abpromise for all tested applications). Antigen: Synthetic peptide: AQKLGVEQPTLYWHVKNKR, corresponding to amino acids 31 - 49 of TetR...
Biotin anti-mouse Ly-49C/F/I/H...
Biology Products: