Navigation Links
TransMessenger Transfection Reagent Handbook

Influence of the RNA Factors such as the size of the RNA and special features contained within it (e.g., IRES, cap, or poly-A tail) can influence its stability and/or the expression rate. Some RNA features may not be compatible with every cell type. Sub-optimal cell
density If cell density at the time of TransMessenger RNA complex addition is not at an optimal
level, cells may not be in the optimal growth phase. This can lead to insufficient uptake of
complexes into the cells or inefficient processing of the RNA of interest. For adherent cells, the optimal confluency for transfection is 8090% (for single-stranded RNA) or 5080% (for siRNA). Be sure to seed cells a minimum of 24 h prior to transfection. Cell typeRNA
combination RNA species with features such as a cap, poly-A tail, or IRES will not necessarily be processed in every cell type. For example, an RNA with an IRES may not be translated in COS-7 and HeLa cells. If no expression is observed change the cell type or, if possible, the RNA. Poor RNA quality RNA used for transfection should be of high quality. Impurities present in RNA preparations can potentially lower transfection efficiency. Degradation of RNA leads to decreased expression levels. RNA should be checked for degradation before transfection. We recommend purifying RNA using RNeasy Kits, Oligotex mRNA Kits, or QIAGEN DNA/RNA Kits. Rep
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/15/2014)... SAN JOSE, California , January 15, 2014 ... antibody-drug conjugates for cancer, today announced the appointment of Thomas ... Reynolds has over 20 years, development experience gained in the ... Genetics. "I am delighted to welcome Tom at ...
(Date:1/15/2014)... 2014 More than 5 million Americans ... in 3 seniors will die with Alzheimer’s or another ... figures have shocked many Americans into looking for ways ... these tragic age-related cognitive disorders. Jonathan Weisman, president of ...
(Date:1/15/2014)... Scottsdale, Arizona (PRWEB) January 15, 2014 ... growth for Scottsdale’s Brain State Technologies®. They saw continued ... University Medical Center who were awarded a $1 million ... papers published in “Brain and Behavior” a peer reviewed ...
(Date:1/14/2014)... 14, 2014 Date: Friday, April 11, 2014 ... Country Club, 1360 Almshouse Road, Warrington, Pa. , ... solely dedicated to finding a cure for hepatitis B and ... host its annual Crystal Ball on Friday, April 11 at ...
Breaking Biology Technology:Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
...  PharmAthene, Inc. (NYSE Amex: PIP ), a biodefense ... today that management will present at the ROTH 24th Annual ... 5:30 pm PT at the Ritz-Carlton Laguna Niguel in Dana ... , Monday, March 12, 2012 at 5:30 pm Place : ...
... Production of energy from the difference between salt water ... but now, using an ammonium bicarbonate salt solution, Penn ... with energy extracted from the salt-water fresh-water gradient to ... each having limitations, and putting them together," said Bruce ...
... SAN DIEGO, Mar.1, 2012 /PRNewswire-iReach/ -- FirstMark, ... meeting of its Scientific Advisory Board, that was held ... with distinguished backgrounds in cardiovascular medicine and research. The ... guidance for further clinical development and validation of FirstMark,s ...
Cached Biology Technology:Unique salt allows energy production to move inland 2Inaugural Meeting of the FirstMark Scientific Board of Advisors 2Inaugural Meeting of the FirstMark Scientific Board of Advisors 3
(Date:4/22/2014)... the University of Montana,s Wildlife Biology Program, co-wrote a research ... National Academy of Sciences on how streamwater chemistry varies ... Lowe and ... of Washington, the Cary Institute of Ecosystem Studies, the University ... examined 664 water samples collected every 10 meters along 32 ...
(Date:4/22/2014)... 2014) Biotechnology scientists must be aware of the ... licensing guidelines, according to a new paper from Rice ... the current issue of the journal Regenerative Medicine ... U.S. Supreme Court ruling in the case Association for ... genes are unpatentable. The court case and rulings garnered ...
(Date:4/22/2014)... expert will present a poster titled "A ... 7th International Nanotoxicology Congress to be held April ... Sharma will outline a strategy consistent with the 2007 ... Toxicity Testing in the 21st Century: A Vision and ... involving human cells and cell lines for mechanistic pathwaybased ...
Breaking Biology News(10 mins):New research focuses on streamwater chemistry, landscape variation 2New patenting guidelines are needed for biotechnology 2New patenting guidelines are needed for biotechnology 3
... for Free-Electron Laser Science have devised a novel way to boil ... concept, which has not yet been demonstrated in practice, could heat ... Celsius in just half a picosecond (a trillionth of a second). ... one picosecond is to a second what one second is to ...
... like Alzheimer,s, Parkinson,s and Creutzfeldt-Jakob disease. But amyloid ... the development of new composite materials for the ... even make objects invisible. Researchers from Chalmers ... about amyloid in an article published in the ...
... Caribou in southern and eastern Canada may disappear from most ... takes the toll on their habitat that scientists predict in ... Nature Climate Change . Scientists looked at reservoirs of ... to stable habitats. They found that caribou populations in the ...
Cached Biology News:Ultrafast heating of water -- This pot boils faster than you can watch it 2Ultrafast heating of water -- This pot boils faster than you can watch it 3Alzheimer substance may be the nanomaterial of tomorrow 2Climate change threatens genetic diversity, future of world's caribou 2
... from non-demolished blood that is ... The donors are not medicated ... maintained on an antibiotic free ... : 85 mg/ml (by Refractometry).Strain: ...
Alkaline Phosphatase (E. coli C75), 100 units. Removes 5'-phosphates from DNA. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzymes, Alkaline Phosphatases....
... cDNA Labeling Kit with CyScribe GFX ... generation and purification of Cy3- and ... for complete cDNA labeling and purification.Flexible ... for performing labeling reactions with either ...
... CyScribe GFX Purification Kit, 24 reactions. ... Cy3- and Cy5-labeled cDNA.Optimized reagents are ... and purification.Flexible and optimized protocols can ... with either Cy3- or Cy5-labeled nucleotides.Reliable ...
Biology Products: