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TransMessenger Transfection Reagent Handbook

her cytotoxicities, or lower gene-silencing effects than expected are observed. Comments and suggestions are listed in the order in which they should be considered. Observation Possible cause Comments and suggestions
Low transfection
efficiency
Sub-optimal
TransMessenger
Reagent:RNA ratio If the ratio of TransMessenger Transfection Reagent to RNA is sub-optimal, the overall
charge of the complexes may be negative, neutral, or strongly positive, which can lead to
inefficient adsorption to the cell surface. Optimize the TransMessenger Transfection
Reagent to RNA ratio according to the optimization guide. Insufficient
TransMessenger
RNA complex If the transfection efficiency is lower than expected and cytoxicity is acceptably low, increase the overall amount of TransMessengerRNA complex added to the cells. Refer to the pipetting scheme in Table 2. Sub-optimal
incubation time for
protein expression Different cell types achieve maximal expression levels at different times post-transfection. This should be kept in mind when determining the length of incubation after transfection. If the time point of maximal expression is not known for a particular cell typeRNA combination, a time course experiment may be necessary.
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