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TransMessenger Transfection Reagent Handbook

interference (RNAi), siRNA, and Transmessenger Transfection Reagent
Optimizing siRNA Transfection
Guidelines for Transfection of siRNA Duplexes Using TransMessenger Transfection Reagent
Troubleshooting Guide
References
Appendix

Kit Contents

0.5 ml TransMessenger Transfection Reagent (1 mg/ml), 0.5 ml Enhancer R (1 mg/ml), 1 x 15 ml Buffer EC-R, sufficient for 60 transfections in 6-well plates, or 80 transfections in 12-well plates.

Storage and Stability

TransMessenger Transfection Reagent, Enhancer R, and Buffer EC-R are supplied as readyto-use solutions and are shipped at ambient temperature without loss in stability. However, they should be stored at 28C upon arrival. The solutions are stable for 1 year at 28C. In contrast to many liposome-based reagents, TransMessenger Transfection Reagent is not sensitive to oxygen, and so does not require storage under an inert gas. Additionally, TransMessenger Transfection Reagent does not need to be stored on ice during the transfection procedure.

Quality Control

Endotoxin levels are <10 EU/ml as determined using a Kinetic-QCL test (BioWhittaker, Inc.). TransMessenger Transfection Reagent is tested by transfection of a CAT-encoding RNA construct into NIH/3T3 cells to ensure lot-to-lot consistency. Microbial limit tests g
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