Navigation Links
TransMessenger Transfection Reagent Handbook

to be transfected and kept constant in future experiments. This is achieved by counting cells prior to seeding and by keeping the time period between seeding and transfection (minimum 24 hours) constant. This will ensure that the cell density is not too high and that the cells are in optimal physiological condition on the day of transfection.

Table 4. Pipetting scheme for optimization of siRNA transfection in 24-well plates

Guidelines for Transfection of siRNA Duplexes Using TransMessenger Transfection Reagent

The procedure below is based on the TransMessenger Reagent standard procedure and RNAi studies using targets described by Elbashir et al. (2). This procedure is provided as a starting point for optimization of siRNA transfection in mammalian cells using TransMessenger Transfection Reagent. For specific cell type and targets, optimal conditions could be different from those described here. Please be sure to read the background information and the protocol notes on pages 9 and 10 of this handbook before starting. 1. The day before transfection, seed 50,000 to 100,000 cells (depending on cell type and the time point of analysis) per well of a 24-well plate in 0.5 ml appropriate growth medium containing serum and antibiotics.
Make sure that cells are in good condition and are seeded 24 h before transfection. Cells should be 5080% confluent on the day of transfection. 2. Incubate cells under their normal growth conditions (typicall
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:9/30/2014)... Proove Biosciences , the commercial and research ... new genetic test built upon research from their published ... will provide physicians with an objective way to understand ... stratified between individuals. With this new test, physicians will ... genetic predisposition that will affect their pain tolerance. , ...
(Date:9/30/2014)...  RegeneRx Biopharmaceuticals, Inc. (OTC: RGRX) ("the Company" ... a Canadian Patent for Inhibition or Reversal of ... claims using thymosin beta 4, various fragments and ... purposes.  The patent will expire in 2021. ... ) RegeneRx is focused on ...
(Date:9/30/2014)... 2014 Fast, semi-automatic creation ... in documents ChemAxon , a ... services for life science research, launches ChemCurator, an ... structures from documents. Markush structures are ... generating and understanding Markush structures is a difficult ...
(Date:9/30/2014)... OriginOil Inc. (OTC/QB: OOIL), developer of Electro ... up large quantities of water, announced today that in ... the launch of the company's CLEAN-FRACâ„¢ industrial grade demonstrator ... STW Resources . , Immediately upon startup, the ... and achieved a 99.8% reduction in Turbidity on flowback ...
Breaking Biology Technology:Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2OriginOil, Inc. Report to Shareholders: Blue Gold in West Texas 2
... Area Technical College is launching a major effort to ... colleges and high schools. The initiative will feature new ... better prepare qualified technicians to work in the fast ... Foundation (NSF) grant - the second such grant MATC ...
... - Alfalight, Inc. , a Madison-based manufacturer ... markets, has been awarded $1.4 million in funding for ... program, sponsored by the Defense Advanced Research Projects ... is to increase a laser's power conversion efficiency -- ...
... Days of summer. Business slows. Indeed, in Europe, where I ... of suspended animation that will last until the first of ... attention. Work's grasp weakens. , ,Or does it? , ,After ... Monday morning mountain in my inbox and a to-do list ...
Cached Biology Technology:Alfalight garners $1.4 million defense funding for diode program 2Leaving it all behind 2
(Date:9/30/2014)... environment on Earth, but when the climate changes and ... limit. The amount of time needed to adjust to ... cope in the future, reveals a new study from ... scientific journal Proceedings of the Royal Society B ... levels of greenhouse gases in the atmosphere. The greenhouse ...
(Date:9/30/2014)... goes wrong with your muscles because of age, disease or ... is. That,s where a new research report published in the ... comes in. In the report, a team of scientists ... help scientists "see" which genes are active in an organ ... activity and that men have approximately 400 more active genes ...
(Date:9/30/2014)... 30, 2014) People who suffer from asthma may ... control their asthma besides properly taking medications and avoiding ... in the Annals of Allergy, Asthma and Immunology ... Allergy, Asthma and Immunology (ACAAI), asthma sufferers can learn ... The woman described in the Annals article improved her ...
Breaking Biology News(10 mins):Shape up quickly -- applies to fish, too! 2Scientists identify which genes are active in muscles of men and women 2Asthma symptoms kicking up? Check your exposure to air pollution 2
... HARBOR, N.Y. Many proteins do not function ... associate to form larger structures called protein complexes. ... ( www.cshprotocols.org/TOCs/toc5_09.dtl ) features a set of methods ... An additional featured article details the generation of viral ...
... Society (ACS) Weekly PressPac from the Office of ... peer-reviewed journals and Chemical & Engineering News ... American Chemical Society as the source for this ... ARTICLE #1 FOR IMMEDIATE RELEASE Gene ...
... the introduction of genetic material into a patient,s cells resulting ... years, it has been shown that gene therapy is a ... for which there is no attractive alternative therapy. Gene ... other diseases that affect heart, brain and even for cancer. ...
Cached Biology News:Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols 2American Chemical Society's weekly PressPac -- April 29, 2009 2American Chemical Society's weekly PressPac -- April 29, 2009 3American Chemical Society's weekly PressPac -- April 29, 2009 4American Chemical Society's weekly PressPac -- April 29, 2009 5American Chemical Society's weekly PressPac -- April 29, 2009 6Moving gene therapy forward with mobile DNA 2
SHEEP ANTI FLECAINIDE...
MOUSE ANTI HUMAN B-CELLS (FMC7):FITC Immunogen: HRIK cells - Human B-Lymphoblastoid line...
... are ideal for use in refrigerators, freezers, or liquid ... vibrant colors allow for color coding to project, user, ... drainage or promote cool air circulation. Supplied with 10 ... tubes. Box dimensions: 13 x 13 x ...
RABBIT ANTI BACTERIAL HISTIDASE...
Biology Products: