Navigation Links
TransMessenger Transfection Reagent Handbook

web site contains siRNA transfection protocols and the most up-to-date information and literature on QIAGEN reagents for RNAi. Visit the Transfection Tools site for answers to your RNAi questions www.qiagen.com/transfectiontools/.

Optimizing siRNA Transfection

To achieve the best results in siRNA transfection we recommend optimizing the following parameters.

Amount of siRNA and Enhancer R
The amount of siRNA used is a critical factor for efficient transfection and gene silencing. The recommended starting amount for transfection of siRNA in 24-well plates is 0.8 g. A pipetting scheme for optimizing the transfection of siRNA in adherent cells in a 24-well format is provided in Table 4 on page 16. Always use a 1:2 ratio of nucleic acid (g) to Enhancer R (l).

Ratio of TransMessenger Transfection Reagent to siRNA
The ratio of TransMessenger Transfection Reagent to siRNA should be optimized for every new cell type and siRNA combination used. As a starting point for optimization we recommend using an siRNA to TransMessenger Transfection Reagent ratio (g:l) of 1:5 when using 24-well plates. To optimize siRNA transfection in 24-well format, prepare separate transfection mixtures using:
The starting point siRNA and TransMessenger Reagent quantities that are given in the protocol on page 16
Half the listed quantities and ratios
Double the listed ratios and quantities.

Cell density at transfection
The optimal confluency for transfection of adherent cells with siRNA is 5080%. The optimal confluency should be determined for every new cell type
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/23/2015)... January 23, 2015 Portal Solutions , ... workplaces on Microsoft Office 365 and SharePoint platforms , ... Award program for being one of the region’s fastest-growing mid-sized ... employee growth. , “To be recognized a second year ...
(Date:1/22/2015)... today announces the firm brokered a long-term lease totaling 202,492 ... company, at Two Ledgemont Center in Lexington, Massachusetts ... and Brian Cohen , senior vice president, represented Shire ... Hayden Ave. Photo - http://photos.prnewswire.com/prnh/20150122/170765 ...
(Date:1/22/2015)... San Diego, California (PRWEB) January 22, 2015 ... R Promotional Offer to their comprehensive portfolio of ... Offer allows customers to purchase a Centrifuge 5424/5424 R ... Pipette Bundle of either Eppendorf Research plus or Eppendorf ...
(Date:1/22/2015)... 2015 Diagenode, Inc., a leading ... Bioruptor® and complete solutions for epigenetics research, recently ... alleviating the need for manual processing. The new ... ChIP of histones or transcription factors and a ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... Bowman Carney &,Williams, PLLC announces an investigation into ... CGPI ). On February 26, 2008,CollaGenex announced that ... an affiliate of Galderma Pharma S.A. Under the ... per share in cash for each,share owned. This ...
... WILL FOCUS ON COLLECTION, PROCESSING AND STORAGE OF EQUINE ... BONE MARROW AND PLACENTAL TISSUE STEM CELLS, RANCHO ... ), a leading supplier of innovative products and,services that ... its,wholly-owned subsidiary, Vantus, Inc., has signed a formal agreement ...
... 27 NuVasive, Inc. (Nasdaq:,NUVA), a medical device company ... the spine, announced today,that Kevin O,Boyle, Executive Vice President ... Canaccord Adams, Small-Cap Orthopedics,Conference at The Mission Bay Conference ... 4, 2008 at 8:10 a.m. PT., A live ...
Cached Biology Technology:ThermoGenesis Subsidiary Vantus Announces Stem Cell Agreement With UC Davis Center For Equine Health 2ThermoGenesis Subsidiary Vantus Announces Stem Cell Agreement With UC Davis Center For Equine Health 3ThermoGenesis Subsidiary Vantus Announces Stem Cell Agreement With UC Davis Center For Equine Health 4ThermoGenesis Subsidiary Vantus Announces Stem Cell Agreement With UC Davis Center For Equine Health 5NuVasive to Present at Canaccord Adams' Small-Cap Orthopedics Conference 2NuVasive to Present at Canaccord Adams' Small-Cap Orthopedics Conference 3
(Date:12/11/2014)... blood pressure plays a role in human health has been ... for high blood pressure – was first described as a ... used in measuring blood pressure was invented in 1896. ... its triggers and its effects. In fact, recent findings have ...
(Date:12/10/2014)... YORK , Dec. 8, 2014 You,ve been here before: ... can,t remember your password, site key or the answer to your ... teacher? Today, Hoyos Labs , a digital ... put an end to the frustration that comes with usernames, passwords ...
(Date:12/10/2014)... 2014  Wake Forest Baptist Medical Center today announced plans for ... Funding for this $50 million capital project is part of ... summer. The medical education building will be ... complex, adjacent to 525@vine in Wake Forest Innovation Quarter. Construction ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... of researchers from the Department of Biochemistry and Molecular ... Autònoma de Barcelona has discovered that tetanus toxin, which ... against psychological disorders such as depression, anxiety and anorexia, ... as Parkinson's disease. , Tetanus toxin is a neurotoxin ...
... grain sorghum , Trials have begun in Kansas on ... of many plastics, drugs, solvents and food additives. Developed ... form of the bacteria E. coli that metabolizes glucose ... to make key chemical intermediates like succinate is a ...
... scientist from the University of Manchester has discovered the first ... up to 20 million years old. , Two droplets of ... amber which also contains the spider - Filistatidae - a ... The droplets are the first identified examples of spider blood ...
Cached Biology News:Tetanus toxin found to have therapeutic properties 2Bacteria are key to 'green' plastics, drugs 2Bacteria are key to 'green' plastics, drugs 3Spider blood found in 20 million year old fossil 2
Anti-Rat KAPPA/LAMBDA:HRP, Clone MARK-1/MARL-15, Monoclonal Antibody...
MOUSE ANTI RAT KAPPA/LAMBDA:HRP Immunogen: MARK-1: Rat kappa light chains (IR202 and IR968)MARL-15: Rat lambda light chains (RH58, IR31)...
Request Info...
Request Info...
Biology Products: