Navigation Links
TransMessenger Transfection Reagent Handbook

lows transfection efficiency to be easily followed.

Designing an siRNA
The design of an siRNA is a critical factor in its ability to mediate gene-specific silencing. QIAGEN provides an siRNA oligonucleotide synthesis service that offers expert advice on the design of an siRNA to achieve optimal gene-silencing effects. For more information visit www.qiagen.com/sirna.

Measuring the gene-silencing effect
The gene-silencing effect can be monitored at the protein level by western blotting, immunofluorescence, gene functional analysis, or FACS. Silencing can also be monitored at the mRNA level by real-time RT-PCR. QIAGEN offers the QuantiTect SYBR Green RT-PCR Kit and the QuantiTect Probe RT-PCR Kit for highly sensitive and specific two-step and one-step real-time RT-PCR analysis. All expression data should be compared to levels of a housekeeping gene to exclude the possibility of non-specific effects.

General recommendations
The guidelines and recommendations below are based on RNAi experiments performed in 24-well plates. Please keep in mind that these are only guidelines, and the efficiency of transfection can be dependent on many different parameters, such as siRNA quality, cell type, passage number, and confluency of the cells at the time of transfection. Variations of these parameters should be considered if varying the amounts and ratios of siRNA and TransMessenger Reagent used does not lead to the desired results. If you would like to discuss optimization of your conditions please contact our Technical Services Department (see inside front cover).

RNAi information online
The QIAGEN Transfection Tools
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:3/27/2015)... , March 27, 2015  CASI Pharmaceuticals, Inc. ... the acquisition, development and commercialization of innovative therapeutics addressing ... market with a commercial focus on China ... and 12 months ended December 31, 2014.  ... million), or ($0.05) per share, for the three months ...
(Date:3/26/2015)... N.J., March 26, 2015  Roka Bioscience, Inc. ... focused on providing advanced testing solutions for the ... results for the three months and full year ... 2014 Financial Results:Revenue for the quarter ended December ... for the fourth quarter of 2013 and $1.5 ...
(Date:3/26/2015)... 26, 2015 /PRNewswire/ - SQI Diagnostics Inc. ("SQI" or ... dealing with certain matters, including the appointment of auditors, ... March 26, 2015 (the "Meeting") was adjourned, as previously ... at 11:00 a.m. ( Toronto time). ... from that previous disclosed. The Meeting will reconvene at ...
(Date:3/26/2015)... 26, 2015  BioNano Genomics, Inc., the leader in ... as chief commercial officer. Salyer has more than ... teams in life sciences. At BioNano, Salyer will oversee ... genome-mapping platform to assemble a comprehensive view of complex ... date, 32 institutions located in the United ...
Breaking Biology Technology:CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 3CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 4CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 5CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 6Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 2Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 3Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 4Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 5Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 6Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 7SQI Diagnostics Inc. Announces Adjournment of Meeting 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3
... veteran brings business development and transaction expertise, ... today,announced the appointment of Paul Truex to its ... CEO of Anthera Pharmaceuticals. Previously he,served as President ... $81 million of institutional capital (including $21000000 from ...
... geographic scales ranging from vast climatic regions of the Earth ... of soil nano-particles in a pinch of soil. , ... properties vary from the summit of a hill down to ... less than a quarter inch in diameter, there is a ...
... 30 /Xinhua-PRNewswire-FirstCall/ -- China,Pharma Holdings, Inc. ("China Pharma" ... and markets generic and branded,bio-pharmaceutical products in China, ... its recently announced financing, issuing five million shares ... per share and three-year warrants,to purchase an aggregate ...
Cached Biology Technology:Trius Appoints Paul Truex to Board of Directors 2Sophisticated soil analysis for improved land use 2China Pharma Holdings, Inc. Closes $10 Million Financing 2China Pharma Holdings, Inc. Closes $10 Million Financing 3
(Date:3/23/2015)... Mar. 23, 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... the growing mobile commerce market, announces its biometric payment technology, ... on CNBC television starting March 30 th . ... in New York markets. ... are excited about our new ad campaign following the recent ...
(Date:3/20/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/rswjb4/hand_geometry ) has announced ... Business Report" report to their offering. This ... Thousands. The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... Annual estimates and forecasts are provided for the ...
(Date:3/20/2015)... , Mar. 19, 2015 Research and Markets ... "Iris Biometrics - Global Strategic Business Report" report to ... for Iris Biometrics in US$ Thousands. The report provides separate ... Japan , Europe , ... , and Latin America . ...
Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... multiple aspects of life on Earth, including extinctions ... to walk on land, sirenians (manatees) in the ... and Edwards Aquifer development as seen through cave ... decomposition, degassing, climate change, tectonics, volcanics, current velocity ...
... 31 March 2009] Researchers at the Swedish medical university Karolinska ... brain,s frontal and parietal parts, to increase the understanding of ... billions of neurons in the brain interact. One of the ... mechanism in the brain,s neuronal network that restricts the number ...
... reason to hate leftovers. A research study appearing in ... Leukocyte Biology ( http://www.jleukbio.org ) sheds light on ... from the United States and The Netherlands show that ... it worse when leftover remnants of bacteria cell walls, ...
Cached Biology News:April 2009 Geology and GSA Today media highlights 2April 2009 Geology and GSA Today media highlights 3April 2009 Geology and GSA Today media highlights 4April 2009 Geology and GSA Today media highlights 5April 2009 Geology and GSA Today media highlights 6April 2009 Geology and GSA Today media highlights 7April 2009 Geology and GSA Today media highlights 8April 2009 Geology and GSA Today media highlights 9April 2009 Geology and GSA Today media highlights 10April 2009 Geology and GSA Today media highlights 11Computer simulations explain the limitations of working memory 2Bad mix of bacterial remnants and genetics leads to arthritis 2
Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts....
... Monoclonal Anti-β-Tubulin IV Recognizes ... C-terminal sequence of β-tubulin isotype ... tubulin isotypes is observed. ascites ... the C-terminal sequence of β-tubulin ...
Porcine Aortic Endothelial Cells (PAOEC) (>500,000 cells)...
Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) (>500,000 cells)...
Biology Products: