Navigation Links
TransMessenger Transfection Reagent Handbook

n. Incubation time is determined by the assay and RNA used.
Note: Compared to DNA transfection, optimal expression levels may be obtained earlier following transfection with RNA. Cells transfected with a chloramphenicol acetyltransferase (CAT) reporter RNA are typically incubated for approximately 24 h post-transfection to obtain maximal levels of protein expression. Table 3. Starting points for optimizing the transfection of adherent cells in different formats * We recommend using medium without serum or antibiotics to avoid possible RNase contamination.
If transfections are performed in 96- or 48-well plates, dilute TransMessenger Transfection Reagent with Buffer EC-R to a total volume of 10 l or 20 l, respectively, before addition to the RNAEnhancer R mixture prepared in step 3.

RNA interference (RNAi), siRNA, and TransMessenger Transfection Reagent

Scientists at QIAGEN have successfully performed RNAi experiments using TransMessenger Transfection Reagent and a gene-specific siRNA (1). Below are some guidelines based on information from current literature (25), and our own experiences.

Controls for RNAi experiments
Controls should always be included in RNAi experiments. An siRNA with a scrambled oligonucleotide sequence can be used as a negative control. Library siRNAs that are directed against human gene products and have been shown to deliver gene-specific silencing in RNAi experiments are available from the QIAGEN siRNA Oligonucleotide Synthesis Service. Fluorescently labeled siRNA al
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:8/28/2015)... 2015 According to a new ... Storage Systems, Thawing Equipment, Alarms), by Biospecimen (Human Tissue, ... End User - Global Forecast to 2020", published by ... USD 3,731.03 Million by 2020 from USD 2,150.48 Million ... 2015 and 2020. Browse 74 Tables ...
(Date:8/28/2015)... ... ... Since the initial launch of FireflySci Inc. (FFS) in early 2015, the company continues to ... such as the Fonz in a lab coat and Large Marge have been posted on ... , FireflySci is proud to be the only cuvette manufacturer is the world ...
(Date:8/27/2015)... , Aug. 27, 2015 The National ... patient,s eyes—and life—were saved by doctors at Vanderbilt University ... with NNFF, NovaBay Pharmaceuticals and Dr. John Crew ... at Seton Medical Center in Daly ... used a breakthrough approach, pioneered by Dr. Crew, that ...
(Date:8/27/2015)... ... 27, 2015 , ... Inc. Magazine released its annual list of the 5,000 ... in growth for the three years through 2014. Being named to the Inc. 5000 ... in the country. , “We are thrilled to make the Inc. 5000 list ...
Breaking Biology Technology:Biopreservation Market Worth 3,731.03 Million USD by 2020 2Biopreservation Market Worth 3,731.03 Million USD by 2020 3Biopreservation Market Worth 3,731.03 Million USD by 2020 4Biopreservation Market Worth 3,731.03 Million USD by 2020 5Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3
... 10 a.m.A summary of the National Nanotechnology Initiative (NNI) ... Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the ... report is a supplement to the Presidents Budget for ... budget request, as well as highlights of planned activities ...
... Sigma-Aldrich and Atlas Antibodies Establish Exclusive Distribution ... ... (Nasdaq:,SIAL) today announced an exclusive partnership with Atlas Antibodies to,distribute Prestige ... and cell biology research communities.,Initially consisting of 1800 antibodies, the library ...
... - Gross Margins Increased from 37% to ... - First Six Months Revenues Increased 82% to $14.9 million and Net Income ... - Growth Driven by New Distribution Partners and Increased Demand for ... Products, CHENGDU, China, ...
Cached Biology Technology:National Nanotechnology Initiative releases its fiscal year 2009 budget and highlights 2Sigma-Aldrich Announces New Prestige Antibodies(TM) Line for Proteomics and Cell Biology Researchers 2Sigma-Aldrich Announces New Prestige Antibodies(TM) Line for Proteomics and Cell Biology Researchers 3Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 2Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 3Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 4Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 5Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 6Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 7Tianyin Pharmaceutical Co., Inc. Announces Second Quarter 2008 Financial Results 8
(Date:8/6/2015)... 6, 2015  Today, U.S. Congressman Mike ... ™ to learn firsthand how Silicon Valley technologies ... is a world leader in multi-factor identity management ... helping federal agencies and commercial organizations achieve new ... "The vast majority of network breaches can ...
(Date:8/5/2015)... , August 5, 2015 ... titled "Facial Recognition Market - Global Industry Analysis, Size, ... global market for facial recognition is forecast to reach ... by increased demand for surveillance systems by civil and ... number of crimes and terrorist activities across the globe ...
(Date:8/3/2015)... 3, 2015 Synaptics, Inc. (Nasdaq: SYNA ... announced that members of the executive management team will ... Global Technology Leadership Forum on Tuesday, August 11, 2015 ... held at the Sonnenalp Resort in Vail, ... include forward-looking information. An audio webcast of the event ...
Breaking Biology News(10 mins):Rep. Honda to Visit Crossmatch in Redwood City 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 3Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 4Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 5Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 6
... -- Stevens Institute of Technology presents Research & Entrepreneurship ... , Schaefer Athletic Center on Stevens campus. R&E ... its array of startup companies and student based research ... Institutes, focus on entrepreneurship and success in engineering solutions ...
... Virginia Tech animal scientist, who in 1957 began breeding ... juvenile body weight, has provided scientists around the world ... traits like growth and reproduction traits that molded ... 8,000 years ago. Paul Siegel, University Distinguished Professor ...
... an advance toward development of a urine test for detecting ... States. Such a test could eventually compliment or even reduce ... The study, which analyzes chemical differences in the urine of ... Journal of Proteome Research , a monthly publication. ...
Cached Biology News:Research & Entrepreneurship Day 2010: Engineering Innovation 2Poultry research leads to breakthrough in genetic studies of animal domestication 2
Custom LNA Oligonucleotide synthesis ,High quality Custom LNA Oligonucleotides are available for a variety of different specialty applications and innovative products. ,Please inquire for further...
... Get better net results - with a ... and data management! The ChemStation Plus NDS ... control, data acquisition and data management, giving ... that meets your labs needs now and ...
... Solubility Service, We deliversoluble protein or ... technology yields the best soluble protein results ... each clone submitted , Powerful results on ... protein Solubility Optimization Service (SOS) uses proprietary ...
... The Schleicher & Schuell BioScience Arraying ... dedicated to custom protein and nucleic ... project teams, comprised of R&D, Engineering, ... closely with collaborators every step of ...
Biology Products: