Navigation Links
TransMessenger Transfection Reagent Handbook

n. Incubation time is determined by the assay and RNA used.
Note: Compared to DNA transfection, optimal expression levels may be obtained earlier following transfection with RNA. Cells transfected with a chloramphenicol acetyltransferase (CAT) reporter RNA are typically incubated for approximately 24 h post-transfection to obtain maximal levels of protein expression. Table 3. Starting points for optimizing the transfection of adherent cells in different formats * We recommend using medium without serum or antibiotics to avoid possible RNase contamination.
If transfections are performed in 96- or 48-well plates, dilute TransMessenger Transfection Reagent with Buffer EC-R to a total volume of 10 l or 20 l, respectively, before addition to the RNAEnhancer R mixture prepared in step 3.

RNA interference (RNAi), siRNA, and TransMessenger Transfection Reagent

Scientists at QIAGEN have successfully performed RNAi experiments using TransMessenger Transfection Reagent and a gene-specific siRNA (1). Below are some guidelines based on information from current literature (25), and our own experiences.

Controls for RNAi experiments
Controls should always be included in RNAi experiments. An siRNA with a scrambled oligonucleotide sequence can be used as a negative control. Library siRNAs that are directed against human gene products and have been shown to deliver gene-specific silencing in RNAi experiments are available from the QIAGEN siRNA Oligonucleotide Synthesis Service. Fluorescently labeled siRNA al
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/22/2015)... Controlled Substance Compliance Services (CSCS), a project managed ... legal requirements around using controlled substances in pharmaceutical research, ... their international operations expand and become increasingly complex, large ... a result of the first phase of the CSCS ...
(Date:1/22/2015)... 2015   Cypher Genomics, Inc., the leading ... SQNM ), the leading molecular diagnostics company, ... noninvasive prenatal tests (NIPT). Through this agreement, Sequenom ... to advance analysis of clinically-relevant fetal sub-chromosomal variants ...
(Date:12/25/2014)... 25, 2014 The report “Service Quality ... Type & Provider Type - Global Advancements, Worldwide Forecasts ... an in-depth analysis and forecasting of revenues. , ... 221 pages and in-depth TOC on “Service Quality Management ...
(Date:12/24/2014)... 24, 2014   BioLife Solutions , Inc. (NASDAQ: ... clinical grade hypothermic storage and cryopreservation freeze ... cells and tissues  ("BioLife" or the "Company"), today announced that ... May 4, 2015 (the "Annual Meeting"). Because the ...
Breaking Biology Technology:Global Compliance Service for Controlled Substances to Expand to China 2Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4BioLife Solutions Sets Date for Annual Meeting of Stockholders 2
... CuraGen Corporation,(Nasdaq: CRGN ) announced today the ... holders of the Company,s 4% Convertible,Subordinated Notes due ... retired a total of $50.9 million of the ... million, reflecting an aggregate discount of,approximately 15.1% off ...
... of five University of Akron researchers has published the ... humidity in PLoS ONE, an open-access, online journal for ... are Dr. Peter Niewiarowski, professor of biology; Stephanie Lopez, ... polymer science; Emily Hagan (undergraduate REU participant, Hiram); and ...
... May 15th at 5 PM ... ... today announced that the Company has postponed its live,webcast and conference call to ... results; this call was originally,scheduled for Tuesday, May 13, 2008, at 5:00 p.m. ...
Cached Biology Technology:CuraGen Repurchases $50.9 Million of 2011 Convertible Debt for $43.2 Million 2CuraGen Repurchases $50.9 Million of 2011 Convertible Debt for $43.2 Million 3
(Date:1/22/2015)... CHARLOTTE, N.C. , Jan. 22, 2015 Infinisource has ... introduction of the G2 model. The G2 sets a higher ... company,s iSolved human capital management solution. With plug-and-play installation, touch ... G2 time clock provides a robust time collection solution for ...
(Date:12/22/2014)... , Dec. 22, 2014 Research and Markets ... addition of the "The Global Watermarking and ... http://photos.prnewswire.com/prnh/20130307/600769 ... digital media watermarking and fingerprinting markets. Watermarking aims ...
(Date:12/19/2014)... Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has announced the addition ... Systems Market" report to their offering. ... security market is estimated to grow at a CAGR of ... a larger share in this market, Canada ...
Breaking Biology News(10 mins):Infinisource's NXG series sets new time clock standard 2The Global Watermarking and Fingerprinting Markets 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3
... On April 15, 2010 , Dr. Stipkala joined a panel ... hosted by the University of South Carolina ,s NanoCenter in ... capitalists from all over the Southeast who are active in the nanotechnology ... Dr. Stipkala and ...
... Bethesda, MD The Genetics Society of America is ... selected for poster awards at the 51st Annual Drosophila ... were selected for first, second and third place awards ... undergraduate. This was the first time that undergraduate student ...
... YORK (April 25, 2010) -- Researchers at the ... Psychiatry at Weill Cornell Medical College discovered that ... behaviors. The genetically altered mice, which behaved much ... disorder (OCD), could help scientists design new therapies ...
Cached Biology News:Dr. Jeremy M. Stipkala, Patent Attorney, Speaks on USC NanoCenter Panel 2Genetics Society of America announces award recipients at 51st Annual Drosophila Conference 2Weill Cornell researchers find that a single gene is responsible for OCD-like behaviors in mice 2Weill Cornell researchers find that a single gene is responsible for OCD-like behaviors in mice 3Weill Cornell researchers find that a single gene is responsible for OCD-like behaviors in mice 4
Request Info...
Recombinant Mouse Wnt-3a...
... 20 structurally and functionally related BMPs, which ... were originally identified as protein regulators of ... involved in embryogenesis and morphogenesis of various ... differentiation, chemotaxis and apoptosis of various cell ...
RABBIT ANTI HUMAN TRRAP (CT) Immunogen: Recombinant C-terminal kinase-related domain of human TRRAP....
Biology Products: