Navigation Links
TransMessenger Transfection Reagent Handbook

ure. Mix by pipetting up and down 5 times, or by vortexing for 10 s. 6. Incubate the samples for 10 min at room temperature (1525C) to allow transfectioncomplex formation. 7. While complex formation takes place, gently aspirate the growth medium from the plate, and carefully wash cells once with sterile PBS using 1.52 times the volume of medium used for cell seeding.
IMPORTANT:
Do not allow the cells to become dry. Minimize the time they are without medium. 8. Add 900 l cell growth medium without serum or antibiotics to the tube containing the transfection complexes. Mix by pipetting up and down twice, then immediately add the transfection complexes drop-wise onto the cells. Gently swirl the plate to ensure uniform distribution of the transfection complexes.
IMPORTANT:
Use medium without serum or antibiotics to avoid RNase contamination. 9. Incubate cells with the transfection complexes for 3 h under their normal growth conditions.
Note:
If no cytotoxic effects are observed, the incubation time can be increased up to 4 h. 10. Remove the complexes from the cells, wash cells once with PBS, then add 2 ml fresh medium containing serum and antibiotics to the cells. 11. Incubate cells under their normal growth conditions to allow for protein expressio
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:5/28/2015)... May 28, 2015 In two different ... L. Sherley, M.D., Ph.D., will present the company’s unique ... In a conference talk, he will describe Asymmetrex ... designs with computer simulation to produce the first-ever technology ... and quality over time in culture. When leading ...
(Date:5/27/2015)... DUBLIN , May 26, 2015 ... addition of the "Global Breast Cancer Monoclonal ... offering. Globally, Roche seems to dominate ... largest inventory. Its block buster molecule, Herceptin has ... years. It was found that breast cancer cells ...
(Date:5/27/2015)... The ALS Therapy Development Institute ... owned subsidiary, Anelixis Therapeutics , $1.5 million toward ... targets the innate immune system. The funding comes from ... from the 2014 social media phenomenon known as the ... million raised directly by the Institute through last year’s ...
(Date:5/27/2015)... THOUSAND OAKS, Calif. , May 27, 2015 /PRNewswire/ ... company, has been awarded a U.S. patent for a ... gene in areas such as research, product development and ... useful in regulating key biosynthetic processes that are the ... to offer other seed companies a commercial license to ...
Breaking Biology Technology:Asymmetrex Will Discuss the Importance of Adult Tissue Stem Cell Time with 3D Tissue Engineers at the 14th Annual World Preclinical Congress in Boston in June 2Asymmetrex Will Discuss the Importance of Adult Tissue Stem Cell Time with 3D Tissue Engineers at the 14th Annual World Preclinical Congress in Boston in June 3Global Breast Cancer Monoclonal Antibodies Market Outlook 2020 2Global Breast Cancer Monoclonal Antibodies Market Outlook 2020 3Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 2Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 3Ceres Awarded Patent for Innovation in Soybean 2Ceres Awarded Patent for Innovation in Soybean 3
... 28 /PRNewswire-FirstCall/ - Angiotech Pharmaceuticals, Inc. (NASDAQ: ... (NASDAQ: ATHX) announced positive results from its phase ... therapy product, administered to individuals following acute myocardial ... heart attack. The study results, which represent at ...
... CLEVELAND, Ohio , ... leading provider of medical education and simulation training,products for ... the company,s continued global expansion. Simbionix has increased,the size ... of its Corporate,Headquarters in Cleveland, OH , and ...
... Researchers have discovered mechanisms critical to interactions between hot ... fusion reactor, part of work aimed at developing coatings ... Fusion powers the stars and could lead to ... plant would produce 10 times more energy than a ...
Cached Biology Technology:Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 2Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 3Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 4Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 5Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 6Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 7Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 8Angiotech Pharmaceuticals and partner Athersys announce positive results from phase I study of MultiStem(R) in heart attack patients 9Simbionix Continues its Global Expansion 2Findings show promise for nuclear fusion test reactors 2Findings show promise for nuclear fusion test reactors 3
(Date:5/19/2015)... 2015 Fingerprint Cards (FPC) has received ... FPC1155 and FPC1035 from one if its module partners. Deliveries ... Q3 2015 and the sensors will be used by smartphone ... for 2015 hereby amount to 740 MSEK to date. In ... MSEK and a number of smaller orders not separately communicated ...
(Date:5/14/2015)... 14, 2015  Verificient Technologies, Inc., a ... online remote proctoring, announced a new alliance ... and creator of the Canvas Learning Management ... companies will benefit from the seamless integration ... As a fully integrated multifactor biometrics behavioral ...
(Date:5/8/2015)... Calif. , May 8, 2015 Synaptics Inc. ... solutions, today announced that members of the executive management team ... Morgan 43rd Annual Technology, Media and Telecom Conference Date: ... Hotel in Boston, MA ... 27, 2015 Time: 2:45pm ET Location: The New York Palace ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Verificient and Instructure Announce Alliance Partnership to Provide Online Proctoring for K-12 and Higher Education Institutions 2Synaptics to Present at Upcoming Investor Conferences 2
... consume foods purchased in vending machines are more likely to ... with being overweight, obese or at risk for chronic health ... research from the University of Michigan Medical School. The ... bars and other related sales that compete with USDA lunch ...
... PASADENA, Calif.Researchers at the California Institute of Technology ... carbon just one atom thickto visualize the structure of ... first direct images of how water coats surfaces at ... potentially unlimited number of other molecules, including antibodies and ...
... A new study from the Center for Interdisciplinary Chronobiological ... additional link between Light At Night (LAN) and cancer. ... out at the University of Haifa that also established ... ,environmental light pollution, which the study has shown is ...
Cached Biology News:Children who eat vended snack foods face chronic health problems, poor diet 2Caltech chemists develop simple technique to visualize atomic-scale structures 2Caltech chemists develop simple technique to visualize atomic-scale structures 3Connection between light at night and cancer revealed in additional study 2