Navigation Links
TransMessenger Transfection Reagent Handbook

ure. Mix by pipetting up and down 5 times, or by vortexing for 10 s. 6. Incubate the samples for 10 min at room temperature (1525C) to allow transfectioncomplex formation. 7. While complex formation takes place, gently aspirate the growth medium from the plate, and carefully wash cells once with sterile PBS using 1.52 times the volume of medium used for cell seeding.
IMPORTANT:
Do not allow the cells to become dry. Minimize the time they are without medium. 8. Add 900 l cell growth medium without serum or antibiotics to the tube containing the transfection complexes. Mix by pipetting up and down twice, then immediately add the transfection complexes drop-wise onto the cells. Gently swirl the plate to ensure uniform distribution of the transfection complexes.
IMPORTANT:
Use medium without serum or antibiotics to avoid RNase contamination. 9. Incubate cells with the transfection complexes for 3 h under their normal growth conditions.
Note:
If no cytotoxic effects are observed, the incubation time can be increased up to 4 h. 10. Remove the complexes from the cells, wash cells once with PBS, then add 2 ml fresh medium containing serum and antibiotics to the cells. 11. Incubate cells under their normal growth conditions to allow for protein expressio
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:8/29/2014)... , Aug. 29, 2014 /PRNewswire/ - iCo Therapeutics ... today reported financial results for the six months ... are expressed in Canadian dollars and presented under ... Quarter 2014 Highlights , Announced top-line ... (VA) primary endpoint for subjects enrolled in the ...
(Date:8/29/2014)... 2014 Three Lawrence Livermore researchers ... Hydrogen Production R&D Award for their research ... using sunlight. , Shared with collaborators from the ... of Nevada, Las Vegas (UNLV), the award recognizes ... photoelectrochemical solar-hydrogen production and corrosion processes. , These ...
(Date:8/29/2014)... 2014 A major challenge before new ... dense realm of Boston-Cambridge, is gaining visibility that can ... Sherley, the Director of Boston’s Adult Stem Cell Technology ... national visibility of his company an important priority since ... addition to a social media marketing campaign ...
(Date:8/28/2014)... SAN DIEGO, California, USA (PRWEB) August 28, 2014 ... at the Department of Physics and Optical Science and ... at the University of North Carolina at Charlotte, and ... has been elected to serve as the 2015 Vice ... photonics. , SPIE 2014 President Philip Stahl announced recent ...
Breaking Biology Technology:iCo Therapeutics Announces Second Quarter 2014 Financial Results 2iCo Therapeutics Announces Second Quarter 2014 Financial Results 3iCo Therapeutics Announces Second Quarter 2014 Financial Results 4Livermore Team Awarded for Hydrogen Production Research 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2
... ... specifically established to treat adolescents and adults and is today the largest of its kind ... , ... The Hertz Family Foundation of Santa Monica has announced that total contributions now exceed $2 ...
... , Opinion ... , WASHINGTON, Dec. 9 On Tuesday, December 8, 2009, ... The Decade-2 Education Forum in Washington, ... to America,s students and was sponsored by Amgen , a leading biotechnology ...
... Tenn., Dec. 9 Cumberland Pharmaceuticals Inc. (Nasdaq: ... agreement with DB Pharm Korea Co. Ltd. , a Korean-based ... in South Korea. Designed to treat pain and fever in the ... Administration and launched by Cumberland in the United States earlier this ...
Cached Biology Technology:Hertz Annual Appeal for Adult Congenital Heart Disease Center At UCLA Passes $2-Million Mark in Funds Raised Over Ten Years 2Hertz Annual Appeal for Adult Congenital Heart Disease Center At UCLA Passes $2-Million Mark in Funds Raised Over Ten Years 3Science Education Experts Discuss Solutions for Global Competitiveness at Scientific American Forum 2Science Education Experts Discuss Solutions for Global Competitiveness at Scientific American Forum 3Cumberland Pharmaceuticals Licenses Caldolor(R) (ibuprofen) Injection to DB Pharm Korea for Commercialization in South Korea 2Cumberland Pharmaceuticals Licenses Caldolor(R) (ibuprofen) Injection to DB Pharm Korea for Commercialization in South Korea 3Cumberland Pharmaceuticals Licenses Caldolor(R) (ibuprofen) Injection to DB Pharm Korea for Commercialization in South Korea 4
(Date:8/28/2014)... is now available from the Lady Bird Johnson Wildflower ... identifying harmful non-native plant, insect and other invasive species ... cause about $137 billion in economic and other damage ... native plants and more. , "By having streamlined tools ... species occur, we,re more likely to catch these threats ...
(Date:8/28/2014)... your neighbors than traditional cigarettes, but still release ... study from USC. , Scientists studying secondhand smoke ... exposure to harmful particles, with close-to-zero exposure to ... harmful metals in second-hand e-cigarette smoke were found ... contains high levels of polycyclic aromatic hydrocarbons ...
(Date:8/28/2014)... Ga. The support of peer groups and ... breastfeeding programs, according to recent University of Georgia ... in the Athens-Clarke County area determined that role ... outcomes of mothers of infants. , "Mothers who ... successful at breastfeeding," said study co-author Alex Anderson, ...
Breaking Biology News(10 mins):Mobile app makes ID of harmful plants, insects in Texas a snap 2Second-hand e-cig smoke compared to regular cigarette smoke 2Breastfeeding study shows need for effective peer counseling programs 2
... For clinicians, the ability to look routinely inside the body ... how it confronts disease is a distant dream. But in ... of researchers from the Howard Hughes Medical Institute (HHMI) at ... step toward realizing that vision by demonstrating the ability to ...
... insect forms—beetles, flies, dragonflies, mosquitoes, grasshoppers, ants, wasps, bees, ... all species known. The large number of insect species ... or more factors: a high rate of formation of ... new environments and exploit new ecological niches, and a ...
... No matter how healthy a life one leads, no person ... though the advances of the modern age may have extended ... genetic limits to longevity. One prominent theory of aging lays ... and proteins by “free radicals,?highly reactive molecules produced by the ...
Cached Biology News:Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method 2Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method 3Islands in the Genome Promote Speciation 2Navigating the brain for sense of direction as paradigm for higher cognitive functions 2
... Isothermal Block is an anodized aluminum, solvent-resistant ... and to provide efficient thermal transfer to ... design of the HT96 Isothermal Block replaces ... product design allowing for compatibility with robotic ...
Human monocyte-derived dendritic cells, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Dendritic Cells and EPCs...
...
...
Biology Products: