Navigation Links
TransMessenger Transfection Reagent Handbook

Procedure 1. The day before transfection, seed 46 x 105 cells (depending on the cell type) per well of a 6-well plate in 2 ml appropriate growth medium containing serum and antibiotics.
Note: Make sure that cells are in good condition and are seeded at least 24 h before transfection. Cells should be 8090% confluent on the day of transfection. 2. Incubate cells under their normal growth conditions (generally 37C and 5% CO2). 3. On the day of transfection, dilute 4 l Enhancer R in Buffer EC-R. Add 2 g RNA (minimum RNA concentration 0.1 g/l) and mix by vortexing for 10 s. The final volume should be 100 l.
For example, if the RNA concentration is 0.5 g/l, dilute 4 l Enhancer R in 92 l Buffer EC-R, then add 4 l RNA solution.
IMPORTANT:
Always mix Enhancer R with Buffer EC-R before adding RNA.
Always keep the ratio of RNA to Enhancer R constant.
Note:

The best results are achieved when RNA of the highest purity is used for transfection. RNA purified with RNeasy Kits and Oligotex mRNA Kits is highly recommended. 4. Incubate at room temperature (1525C) for 5 min, then spin down the mixture for a few seconds to collect drops from the top of the tube. 5. Add 8 l TransMessenger Transfection Reagent to the RNAEnhancer R mixt
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:8/20/2014)... GraphDB™ 6.0 from ... the enterprise replication cluster, faster loading speeds, higher ... Elasticsearch. This release happens to coincide with a ... GraphDB™ was formerly known as OWLIM. ... enterprise resilient RDF triplestore will also benefit from ...
(Date:8/19/2014)... Cellgen Diagnostics today ... fund a corporate lab for its genetic-based medical ... is a critical component in the move from ... implementation of personalized medicine – a more predictive, ... Indiegogo contributions will support Cellgen’s intent to develop ...
(Date:8/19/2014)... 2014 CSSi, the leader in patient recruitment ... formation of the company,s Medical and Clinical Advisory Board ... Dr. William E. Gannon, Jr. to the ... The MCAB, with Dr. Gannon,s leadership, will ... and set strategic goals for the advancement and development ...
(Date:8/18/2014)... 19, 2014 2014 Deep Research ... a professional and in-depth research report on the ... information, including its definition, classification, application, and ... This research covers the international market analysis, including ... analysis covering macroeconomic environment & economic situation analysis. ...
Breaking Biology Technology:Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 2CSSi Appoints Dr. William E. Gannon, Jr. to Newly Formed Medical and Clinical Advisory Board 3Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 2Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 3Linalool Industry & Diphenylamine Market (Global, China) 2014 Analysis Now Available at DeepResearchReports.com 4
... Microfluidic Systems (MFSI),along with its manufacturing ... invited to attend the U.S. House of,Representatives ... and Responding to Bioterrorism. MFSI will be ... The purpose,of the fair is to educate ...
... Memory,Pharmaceuticals Corp. (Nasdaq: MEMY ) today ... included an explanatory,paragraph in their opinion on the ... Report on Form 10-K for the year ended,December ... continue as a going,concern. The Company discusses this ...
... today,announced it has secured $20 million in venture ... includes Silicon Valley Bank,and Comerica Bank. Aerovance drew ... The remaining $10 million is available upon the ... interest and enthusiasm in Aerovance,and our clinical programs," ...
Cached Biology Technology:Memory Pharmaceuticals Receives Audit Opinion Containing Going Concern Qualification 2
(Date:8/20/2014)... WORCESTER, MA Using population-based screening outcomes of approximately ... states, including four researchers at the University of Massachusetts ... combined immunodeficiency (SCID) can be successfully implemented across public ... programs published in the Aug. 20 issue of the ... JAMA ) showed the rate of SCID in newborns ...
(Date:8/20/2014)... are athletes who have suffered concussions ready to return ... found that high school athletes who head back on ... a significant regression in their abilities to simultaneously walk ... seen in changes in their balance and/or altered walking ... of the 12 had returned to activity in less ...
(Date:8/20/2014)... plastic bags could one day be made out of ... are now reporting. The novel process they developed and ... its agricultural and plastic waste problems, appear in the ... S. Bayer and colleagues at the Italian Institute of ... In 2012, its production reached 288 million tons worldwide, ...
Breaking Biology News(10 mins):Newborn screening expansion offers early diagnosis and treatment to infants with SCID 2Newborn screening expansion offers early diagnosis and treatment to infants with SCID 3Recovery reversal seen in Oregon study of returning concussed athletes 2Recovery reversal seen in Oregon study of returning concussed athletes 3
... be processed unconsciously when the area of the brain ... down, according to research at Rice University in Houston. ... in the Proceedings of the National Academy of Sciences' ... than one pathway along which visual information can be ...
... have identified some of the key factors that prevent ... (MS), complications of premature birth, and other diseases and ... nervous system fails to repair itself and suggest ways ... be reversed. The research is published in the August ...
... an alien if you encountered it on the street tomorrow? ... rather was created in a laboratory right here on Earth ... Peter Ward has the beginnings of an answer. In a ... expanded "tree of life," or biological classification system, to account ...
Cached Biology News:Out of sight, out of mind? Not necessarily 2OHSU researchers discover potential mechanism to repair brain damage linked to Multiple Sclerosis 2OHSU researchers discover potential mechanism to repair brain damage linked to Multiple Sclerosis 3OHSU researchers discover potential mechanism to repair brain damage linked to Multiple Sclerosis 4New book expands biological classifications to account for 'alien' life 2New book expands biological classifications to account for 'alien' life 3
Acid phosphatase (Potato). Monospecific by IEP. Some cross reactivity between Acid phosphatases of other species may occur....
... Killer Cell Receptor-P1, clone 1C10 ... Hybridoma produced by the ... mice immunized (BALB/c X C57BL/6)F1 ... and mouse myeloma cellline. ...
Rabbit polyclonal to ZFP200 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGKSFNHKTNLNKHER (Human) Entrez Gene ID: 7752 Swiss Protein ID: P98182...
Rabbit polyclonal to Leucyl tRNA synthetase ( Abpromise for all tested applications). Antigen: L930-H1176 denatured human recombinant LRS. Entrez Gene ID: 3926 Swiss Protein ID: Q9P2J5...
Biology Products: