Navigation Links
TransMessenger Transfection Reagent Handbook

addition to the cells, wash the cells with PBS, and add fresh medium. If no cytotoxic effects are observed, the incubation time can be increased up to 4 hours.

Table 2. Pipetting scheme for optimizing the transfection of adherent cells in 6-well plates
Protocol for Transfection of Adherent Cells with RNA

The following protocol is for transfection of adherent cells using single-stranded RNA in one well of a 6-well plate. For transfection of siRNA, see page 14. As a starting point, use 2 g RNA for transfection in 6-well plates. Starting points for optimizing transfection in other formats are listed in Table 3 on page 13. See Table 1 on page 9 for the recommended number of cells to seed. Optimal transfection conditions should be determined for every cell typeRNA combination to obtain the highest transfection efficiency with TransMessenger Transfection Reagent.

Important notes before starting
We strongly recommend reading this handbook carefully before starting. Check the integrity and functionality of the RNA (see RNA quality). The RNA should be capable of being efficiently translated in the cell type used for transfection (e.g., IRES compatibility; see RNA structure).
The cells should be in optimal physiological condition on the day of transfection. Subculture the cells a minimum of 24 hours before transfection. The ptimal confluency for transfection is 8090%.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:9/2/2015)... DUBLIN , Sep. 02, 2015 ... ) has announced the addition of Jain PharmaBiotech,s ... and Companies" to their offering. ... variations, development of sequencing technologies, and their applications. ... well as companies developing them. Various applications of ...
(Date:9/2/2015)... 2015 Research ... the addition of Jain PharmaBiotech,s new report ... to their offering. The ... fluorescence in situ hybridization (FISH), comparative genomic ... includes application of nanobiotechnology, microarrays, real-time polymerase ...
(Date:9/2/2015)... -- Aytu BioScience, Inc. (OTCQB: AYTU), a specialty healthcare ... conditions, announced today that it has closed the final ... raising a total of $5.175 million, which includes proceeds ... Josh Disbrow , Chief Executive Officer of ... to be used to conduct clinical studies for both ...
(Date:9/2/2015)... ... 02, 2015 , ... Advancing in medical imaging processing technology ... monitoring in ICU to 3D skull printing analysis for Neurosurgery, ONYX ZEUS all-in-one ... with simultaneous access to high precision medical images and electronic health records. ...
Breaking Biology Technology:Global DNA Sequencing Technologies Report 2015: Profiles of 130 Companies Involved in Sequencing and their 140 Collaborations 2Global Cytogenetics Technologies Study 2015-2025 - Latest Markets and Companies Analysis 2Global Cytogenetics Technologies Study 2015-2025 - Latest Markets and Companies Analysis 3Aytu BioScience Completes $5.175 Million Private Placement 2Aytu BioScience Completes $5.175 Million Private Placement 3Surgical Workstation Powered by 6th Gen. Intel® Core™ Skylake Processors 2Surgical Workstation Powered by 6th Gen. Intel® Core™ Skylake Processors 3
... Fred Alger Management, Inc. (Alger),a leading asset ... M.D. as Vice President, Senior Analyst/Co-Portfolio Manager for,Alger ... and,pharmaceutical industries., David received his Doctor of ... was Chief Medical Resident. While in Pennsylvania David,served ...
... obsolete? EnviroChip™ white papers show how consumers like you can ... damaging your engine. , ... Denver (PRWEB) July 28, ... that EnviroChip™, a revolutionary fuel harmonizing microchip, can eliminate a consumer,s ...
... University of Pennsylvania have created a one-step, repeatable ... or motifs with adjustable features, size and shape ... took advantage of the elastic instability of a ... When exposed to a solvent, circular pores ...
Cached Biology Technology:David Farhadi, M.D. to Join Alger as Vice President, Senior Analyst/Co-Portfolio Manager 2Penn researchers demonstrate a flexible, 1-step assembly of nanoscale structures 2Penn researchers demonstrate a flexible, 1-step assembly of nanoscale structures 3
(Date:8/18/2015)... Aug. 17, 2015 Research and Markets ... the "Global Biometric Authentication & Identification Market: ... & Forecast, 2015-2020" report to their offering. ... identification systems market is expected to grow at ... 2020 and generate over $25 billion (approximately) by ...
(Date:8/12/2015)... 12, 2015  Synaptics Inc. (NASDAQ: SYNA ... today announced that four of its fingerprint ID ... officially been named FIDO Certified™ by the Fast ... certification, Synaptics, Natural ID™ fingerprint solutions are fully ... (UAF) standard and are interoperable among other products ...
(Date:8/6/2015)... and TELTOW, Germany , ... 2015 Siggraph Conference, SensoMotoric Instruments (SMI) shows ... reality , based on Epson,s Moverio BT-200 ... tracking platform. With this new solution, unprecedented quality and ... hands-free interaction with context-sensitive displays. For the first time, ...
Breaking Biology News(10 mins):Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3
... Sperm whales weigh up to 50 tons, and the smallest ... the same success story: They both have developed the ability ... Now Danish researchers show that the biosonar of toothed ... they live in very different environments and vary extremely in ...
... that glioma is derived from brain tumor stem cells, ... are still unclear. The potential of neural stem cells ... been considered, but has not been confirmed. A team ... Hospital of Nanjing Medical University, China found that doxycycline-induced ...
... active immunization in Alzheimer,s disease can increase the ... but it is easy to induce meningoencephalitis, which ... To enhance anti-Aβ antibody generation and induce a ... from the First Affiliated Hospital of China Medical ...
Cached Biology News:Bats and whales behave in surprisingly similar ways 2Bats and whales behave in surprisingly similar ways 3
... (PPase-2A) is a serine/threonine phosphatase isolated from ... as the heterodimer of 60kDa (A) and ... to dephosphorylate the a-subunit of phosphorylase kinase. ... broad substrate specificity and may play a ...
Immulon 2 HB 1x8 Strip assembled, C-bottom; high binding irradiated surface to provide increased binding affinity for hydrophilic proteins and complexes....
... The RVT405DDA Refrigerated Vapor Trap ... of high boiling solvents such as ... DDA™ systems. Its constant temperature refrigeration ... creates optimum cryopumping conditions for DMSO, ...
96 Well Plate EB, RE bottom; high binding irradiated surface to provide increased binding affinity for hydrophilic proteins and complexes. Guaranteed coefficient of variation (CVs)....
Biology Products: