Navigation Links
TransMessenger Transfection Reagent Handbook

s 2 g. A pipetting scheme for optimizing the transfection of adherent cells in 6-well plates is provided in Table 2, page 11. For transfection using other culture formats, please refer to Table 3

Amount of Enhancer R
The RNA (g) to Enhancer R (l) ratio of 1:2 given in the pipetting scheme on page 11 should not be changed. Efficient condensation of RNA with Enhancer R is determined by the mass quantity of RNA.

Ratio of TransMessenger Transfection Reagent to RNAEnhancer R mixture
The overall charge of the TransMessengerRNA complex is determined by the ratio of TransMessenger Transfection Reagent to RNAEnhancer R mixture. Optimal binding of TransMessengerRNA complexes to negatively charged groups (e.g., sialylated glycoproteins) on the cell surface requires a slightly positive net charge. The ratio of TransMessenger Transfection Reagent (l) to RNA (g) is an important factor to optimize for every new cell type and RNA used. As a starting point for optimization we recommend using an RNA:TransMessenger Transfection Reagent ratio of 2 g RNA to 8 l TransMessenger Reagent when using 6-well plates. A pipetting scheme for optimizing the transfection of adherent cells in 6-well plates is provided in Table 2. To optimize transfection in other culture formats, prepare separate transfection mixtures using:
The starting point RNA and TransMessenger Transfection Reagent quantities listed in Table 3,
Roughly half the listed ratios and quantities
Double the listed ratios and quantities.

Incubation period with TransMessengerRNA complexes
Remove TransMessengerRNA complexes 3 hours after their
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Related biology technology :

1. TransMessenger Transfection Reagent
2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
3. TransMessenger Transfection Reagent
4. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TransMessenger Transfection Reagent Handbook

(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA ... disease. One of these latent viruses is the Epstein ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... by the theory, a study found that RA patients ... (1). , What is Microcompetition? , Dr. Hanan ...
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... of high throughput research solutions, today announced that ... companies, has received delivery of Freeslate’s CM ... form screening. , Lupin, headquartered in Mumbai, ... quality, affordable generic and branded formulations and Active ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, ... p.m. , Location: Warrington Country Club, 1360 Almshouse Road, ... Foundation, the only national nonprofit organization solely dedicated to ... quality of life for those affected worldwide, will host ... Warrington Country Club in Warrington, Pa. , Each ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider ... major scientific, regulatory, business, and media events in ... , today announced its former associate Virginia Cox , ... office. Virginia Cox re-joins ... Commissioner for the Office of External Affairs at the U.S. ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
... Calif., Oct. 23 CV Therapeutics,Inc. (Nasdaq: ... data,from a prospectively identified analysis of patients with ... for presentation at the American,Heart Association (AHA) Scientific ... on November 4 - 7, 2007. Ranexa(R) ...
... RESTON, Va., Oct. 23 PRA International,(Nasdaq: PRAI ... senior,director of therapeutic expertise and a specialist in oncology ... research in oncology on Thursday, Nov. 8, 2007 at ... Dr. Berger will,recommend ways to mitigate risk in terms ...
... Flagship Ventures Backing Leader in Fluorescence In ... ... Medical, Inc., a leader in,fluorescence in vivo imaging from research through ... Ventures,joined Flagship Ventures to co-lead this $7 million investment round, which,also ...
Cached Biology Technology:CV Therapeutics Announces Presentations at American Heart Association Scientific Sessions 2007 2CV Therapeutics Announces Presentations at American Heart Association Scientific Sessions 2007 3PRA International to Present Audioconference on Oncology Research - Nov. 8 2VisEn Medical Raises $7 Million in Series B Financing 2VisEn Medical Raises $7 Million in Series B Financing 3
(Date:4/16/2014)... looks like one seamless surface; however, up close, that ... of cells in a variety of shapes and sizes. ... own unique forms, Caltech biologist Elliot Meyerowitz, postdoctoral scholar ... factors in pavement cells, which are puzzle-piece-shaped epithelial cells ... that these unusual shapes were the cell,s response to ...
(Date:4/16/2014)... densely packed array of finger-like projections called microvilli ... line our intestines., Vanderbilt University researchers have now ... which is critical for absorbing nutrients and defending ... the journal Cell , reveal a role ... increase our understanding of intestinal pathologies associated with ...
(Date:4/16/2014)... Geologic and soil processes are to blame for ... according to a study published recently in the ... 842 soil samples from all corners of Ohio showed ... screening level of concern recommended by the U.S. Environmental ... say the authors, who note that regulatory levels typically ...
Breaking Biology News(10 mins):For cells, internal stress leads to unique shapes 2Vanderbilt researchers discover how intestinal cells build nutrient-absorbing surface 2Vanderbilt researchers discover how intestinal cells build nutrient-absorbing surface 3Significant baseline levels of arsenic found in Ohio soils are due to natural processes 2Significant baseline levels of arsenic found in Ohio soils are due to natural processes 3Significant baseline levels of arsenic found in Ohio soils are due to natural processes 4
... IriTech, Inc., a leading provider of iris-based biometric ... the National Institute of Science and Technology (NIST) ... operational iris images. NIST,s results show IriTech,s patented ... factors critical for successful operation. , The report ...
... [PRESS RELEASE, 2 October 2009] A derivative of ... according to a study from the Swedish medical university ... journal Cell Stem Cell , can help scientists ... The study was led by Professor Ernest Arenas and ...
... meant invasive species have been able to populate parts of ... biodiversity there over thousands of years. Now, an international team ... on bird populations on Mediterranean islands. Despite the rat,s environmental ... over time by its enforced cohabitation with the rat. ...
Cached Biology News:NIST Tests Identify IriTech as Iris Recognition Leader 2NIST Tests Identify IriTech as Iris Recognition Leader 3Black rat does not bother Mediterranean seabirds 2
... ELISA Development kit contains the basic components ... to measure natural and recombinant canine Monocyte ... supernates and serum.1 Each kit contains sufficient ... 96-well plates, provided that the following conditions ...
Mouse monoclonal [LPL4A.1] to Plastin L ( Abpromise for all tested applications). SwissProtID: P13796...
Component of HyperMu™ Insertion Kit...
Recombinant Rhesus Macaque IFN-alpha...
Biology Products: