Navigation Links
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells


Integrated system simplifies and improves Northern blot analysis

Karen Dolter Jeff Braman
Stratagene

Streamline RNA analysis with a whole host of products from Stratagene. First, the StrataPrep total RNA miniprep kit is used to isolate ample yields of high-quality RNA from tissues and cells, then the RNA is ready to use in a number of applications, including Northern blot analysis. This procedure features the following products: the Joule Box minigel electrophoresis system, the Stratalinker UV crosslinker,* the prostar first strand RT-PCR kit, Stratagenes primer sets for RT-PCR, the StrataPrep PCR purification kit or the StrataPrep DNA gel extraction kit, the Prime-It II random primer labeling kit, NucTrap probe purification columns,** QuikHyb hybridization solution, and the PersonalHyb hybridization oven. When used together for Northern blot analysis, this excellent integrated system permits high sensitivity and reproducible results.

Northern blot analysis of gene transcription is dependent on such factors as isolating intact, high-quality RNA, probe-labeling to high specific activity, removing unincorporated radiolabeled nucleotides, and hybridizing with conditions that maximize the signal-to-noise ratio. Products developed at Stratagene eliminate many variables that lead to poor quality Northern blot results. Consequently, we used these products for a Northern blot analysis of MSH2 gene transcription in a hamster cell model system.

MSH2 is important for DNA mismatch repair, and mutations in this gene are responsible for many cases of hereditary nonpolyposis colorectal cancer.1 The MSH2 gene encodes a 3.1-kb mRNA that is expressed at low to moderate levels.2 The larger-than-average size and
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Tools for Proteomic Science II
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. New Tools for Small RNA Analysis
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
8. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
9. Detecting miRNA & siRNA
10. Detecting Attomole Amounts of Small RNA
11. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tools for Detecting MSH2 Expression Chinese Hamster Ovary Cells

(Date:12/19/2014)... Today, TiFiber, Inc. announced ... and future company headquarters in Fort Smith, Arkansas ... that safely and effectively control dangerous microorganisms. Fort ... historic, strong support for manufacturing, but also due ... technology-based companies such as TiFiber. , In ...
(Date:12/19/2014)... In contrast to traditional LED lights where ... Valoya uses proprietary LED technology to incorporate their spectra ... which is critical in low proximity installations. The Valoya ... its lifetime which is up to four times longer ... investment cost was clearly higher when compared to traditional ...
(Date:12/19/2014)... , Dec. 18, 2014   ISN ... & Safety Performance Indicators publication series. With ... of activity reported in 2013 within its online contractor ... and safety key performance indicator (KPI) statistics. ... Clients, U.S. publication is available in two ...
(Date:12/17/2014)... TX (PRWEB) December 17, 2014 Achim ... and administration for Stoller Group. In this position, Noack ... Group, on administrative matters, develop marketing strategy and supervise ... services. , “Achim has tremendous knowledge and experience ... of Stoller Group. “His experience and innovative thinking will ...
Breaking Biology Technology:Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 2Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 3Technology Company TiFiber™ to Establish Production Facility in Fort Smith, Arkansas with Support from Local Investors 4ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3Achim Noack Joins Stoller Group as Global Vice President of Marketing and Administration 2
... Oncology Inc., a molecular diagnostics company specializing in ... for gynecologic cancer, announced today that it has ... The Moffitt Cancer Center, the only National Cancer ... agreement provides OvaGene with exclusive worldwide rights to ...
... Codexis, Inc. (Nasdaq: CDXS ) today ... and Chief Marketing Officer.  In this new position, Mr. ... and communications.  He will report to Alan Shaw, Ph.D., ... is a widely recognized marketing executive in the global ...
... Dec. 2, 2011  Immune Design Corp. announced today ... as its new Chief Development Officer.  Dr. Gombotz ... to this key leadership position at Immune Design. ... Immune Design,s therapeutic vaccine product candidates. ...
Cached Biology Technology:OvaGene Oncology Collaborates With Moffitt Cancer Center to Develop and Commercialize microRNAs Associated With Chemotherapeutic Drug Response 2OvaGene Oncology Collaborates With Moffitt Cancer Center to Develop and Commercialize microRNAs Associated With Chemotherapeutic Drug Response 3Codexis Names Wes Bolsen VP and Chief Marketing Officer 2Immune Design Corp. Appoints Wayne R. Gombotz, Ph.D. as Chief Development Officer 2
(Date:12/10/2014)... , December 9, 2014 ... ITN,s BCARD Platform   ... enterprise meeting scheduling solutions for business-to-business (B2B) events, ... world leader in mobile near-field communication (NFC), Bluetooth ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow ...
(Date:12/5/2014)... -- Tute Genomics, a leader in cloud-based solutions for genome ... led by UK-based Eurovestech. Peak Ventures and a number ... "We are at a pivotal time in ... seeks new approaches for the diagnosis, treatment and prevention ... and CEO of Tute Genomics. "One of the main ...
(Date:12/3/2014)... 2014   Marvin Test Solutions , a ... for military, aerospace, and manufacturing organizations, today announced ... TS-900 PXI semiconductor test platform . The ... systems to customers at a fraction of the ... ATE. "Our semiconductor customers asked ...
Breaking Biology News(10 mins):Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3
... Methanosarcina acetivorans lives off everything it can metabolize ... is not yet clear. Scientists at the Ruhr-Universitt Bochum ... USA have identified a protein that might act as ... and found both similarities and differences to the system ...
... animal research published in Biological Psychiatry suggests ... learning that is important clinically. Selective serotonin reuptake ... widely used to treat depression, as well as a ... drugs on learning and memory are poorly understood. ...
... In India most people who are HIV positive don,t ... available. It would therefore meet international standards of cost-effectiveness ... every person in the billion-plus population every five years according ... One . The findings are based on a careful ...
Cached Biology News:How Archaea might find their food 2Do antidepressants impair the ability to extinguish fear? 2Cost-effective: Universal HIV testing in India 2Cost-effective: Universal HIV testing in India 3Cost-effective: Universal HIV testing in India 4
... Many modifications of Eagle's medium ... original formulation. The most widely ... Eagle's,Medium. DME is a modification ... [MEM] that contains a higher ...
... The EpiTect Bisulfite Kit enables ... uracils in less than 6 hours. ... innovative protection against DNA degradation and ... to guarantee fast and reliable results ...
Rabbit polyclonal antibody to Glutathione-S-Transferase (GST)...
... gold standard for automated bioresearch: Developed ... applications • Based on decades of ... accessories designed for bioresearch • Holographic ... Versatility, reliability and ease-of-use • Pulsed ...
Biology Products: