Navigation Links
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells


Integrated system simplifies and improves Northern blot analysis

Karen Dolter Jeff Braman
Stratagene

Streamline RNA analysis with a whole host of products from Stratagene. First, the StrataPrep total RNA miniprep kit is used to isolate ample yields of high-quality RNA from tissues and cells, then the RNA is ready to use in a number of applications, including Northern blot analysis. This procedure features the following products: the Joule Box minigel electrophoresis system, the Stratalinker UV crosslinker,* the prostar first strand RT-PCR kit, Stratagenes primer sets for RT-PCR, the StrataPrep PCR purification kit or the StrataPrep DNA gel extraction kit, the Prime-It II random primer labeling kit, NucTrap probe purification columns,** QuikHyb hybridization solution, and the PersonalHyb hybridization oven. When used together for Northern blot analysis, this excellent integrated system permits high sensitivity and reproducible results.

Northern blot analysis of gene transcription is dependent on such factors as isolating intact, high-quality RNA, probe-labeling to high specific activity, removing unincorporated radiolabeled nucleotides, and hybridizing with conditions that maximize the signal-to-noise ratio. Products developed at Stratagene eliminate many variables that lead to poor quality Northern blot results. Consequently, we used these products for a Northern blot analysis of MSH2 gene transcription in a hamster cell model system.

MSH2 is important for DNA mismatch repair, and mutations in this gene are responsible for many cases of hereditary nonpolyposis colorectal cancer.1 The MSH2 gene encodes a 3.1-kb mRNA that is expressed at low to moderate levels.2 The larger-than-average size and
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Tools for Proteomic Science II
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. New Tools for Small RNA Analysis
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
8. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
9. Detecting miRNA & siRNA
10. Detecting Attomole Amounts of Small RNA
11. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tools for Detecting MSH2 Expression Chinese Hamster Ovary Cells

(Date:3/3/2015)... 3, 2015 /CNW/- SQI Diagnostics Inc. (TSX:V: SQD), a life ... and products for advanced microarray diagnostics, today announced ...  to its Management team. In his capacity as ... transition from a development stage to a commercially ... the Company,s capital market strategy and to secure ...
(Date:3/3/2015)... (PRWEB) March 03, 2015 Crown Bioscience, ... company, has launched a new Life Science division specializing ... preclinical research. , The first products released include ... controls, and soon to be released in vivo grade ... continue to expand their life science portfolio with additional ...
(Date:3/3/2015)... Milpitas, Calif. (PRWEB) March 03, 2015 ... risk management excellence were honored by the Global ... annual event which took place on Wednesday, February 25th, ... , The GSCRC was formed to bring together supply ... thought leaders to advance the professional discipline through opportunities ...
(Date:3/3/2015)... 2015  Thingee Corporation, a software development company for ... entitled, "Technology Adoption: Making Reps, Lives Easier" during the ... Technology ,  in Deerfield Beach, Florida ... Audiences looking to learn how technology can help them ... in the audience for the Thingee presentation. ...
Breaking Biology Technology:SQI Diagnostics Appoints Chief Financial Officer 2SQI Diagnostics Appoints Chief Financial Officer 3Crown Bioscience Launches Life Science Product Catalog 2Global Supply Chain Resiliency Council Names Award Winners 2Global Supply Chain Resiliency Council Names Award Winners 3Global Supply Chain Resiliency Council Names Award Winners 4Thingee to present at 11th Annual Synergistix Users Conference 2
... 12 Abbott today is,announcing its full-year earnings-per-share ... earnings-per-share guidance of $3.31 to $3.33,excluding specified items. ... share,of $3.65 to $3.70 under Generally Accepted Accounting ... midpoint of this 2009 guidance reflects double-digit,growth over ...
... -- American Oriental,Bioengineering, Inc. (NYSE: AOB ... and manufacturer of pharmaceutical and healthcare products in,China, ... undertake one-on-one,meetings at the 2009 UBS Greater China ... Management is currently scheduled to meet with,institutional ...
... MAP,Pharmaceuticals, Inc. (Nasdaq: MAPP ) today announced ... Dose Budesonide (UDB) for children,with asthma and MAP0004 for ... have completed the 12 week treatment period in the ... currently expects to complete,enrollment in its initial Phase 3 ...
Cached Biology Technology:Abbott Announces Earnings Guidance for 2009 2MAP Pharmaceuticals Provides Update on Progress of Two Phase 3 Clinical Programs 2MAP Pharmaceuticals Provides Update on Progress of Two Phase 3 Clinical Programs 3
(Date:1/22/2015)... 13, 2015  Today, FreeWavz ( www.FreeWavz.com ), the ... campaign on Fundable, https://www.fundable.com/freewavz . FreeWavz will ... capacity to meet customer demand. Logo ... http://photos.prnewswire.com/prnh/20150113/168790 Invented by Dr. ...
(Date:1/22/2015)...  BellBrook Labs, a leader in high throughput screening ... a TR-FRET (time resolved Forster resonance energy transfer) ... high throughput screening assay for glycosyltransferases and other ... sensitive detection of hundreds of human and microbial ...
(Date:1/22/2015)... Inc. (NASDAQ: NXTD and NXTDW) a biometric authentication company ... of the Wocket™ smart wallet at CES 2015 in Las ... one of the "11 Hot Products at CES" in a review published ... Far" by Newseveryday.com and "The top 10 gadgets from CES 2015" by ...
Breaking Biology News(10 mins):FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3
... of Fame awards will be presented on April 3 at ... Hall of Fame, established in 1981, serves as a posthumous ... other individual who has been an active member of the ... This year,s awards will honor Fred Winsberg, MD, Martin Resnick, ...
... report that IDO, an enzyme found throughout the body and ... fact essential to the onset of depressive symptoms sparked by ... the Journal of Immunology , is the first to ... induces depressive symptoms in some cases of chronic inflammation. ...
... CHAMPAIGN, Ill. As school districts across the nation ... "No Child Left Behind" Act, opportunities for children to ... Future mandates, however, might be better served by ... study suggesting the academic benefits of physical education classes, ...
Cached Biology News:AIUM presents 2009 Memorial Hall of Fame awards 2Team identifies a molecular switch linking infectious disease and depression 2Team identifies a molecular switch linking infectious disease and depression 3Physical activity may strengthen children's ability to pay attention 2Physical activity may strengthen children's ability to pay attention 3Physical activity may strengthen children's ability to pay attention 4
...
... new application for DNA and protein ... Expression makes complex computational analyses of ... the very latest computing technology to ... sequences are analysed. Features include: Sequence ...
... Solution is a comprehensive suite of ... specifically designed to reliably extract biological ... genomic studies containing thousands of samples, ... efficient and will analyze large data ...
...
Biology Products: