Navigation Links
Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells


Integrated system simplifies and improves Northern blot analysis

Karen Dolter Jeff Braman
Stratagene

Streamline RNA analysis with a whole host of products from Stratagene. First, the StrataPrep total RNA miniprep kit is used to isolate ample yields of high-quality RNA from tissues and cells, then the RNA is ready to use in a number of applications, including Northern blot analysis. This procedure features the following products: the Joule Box minigel electrophoresis system, the Stratalinker UV crosslinker,* the prostar first strand RT-PCR kit, Stratagenes primer sets for RT-PCR, the StrataPrep PCR purification kit or the StrataPrep DNA gel extraction kit, the Prime-It II random primer labeling kit, NucTrap probe purification columns,** QuikHyb hybridization solution, and the PersonalHyb hybridization oven. When used together for Northern blot analysis, this excellent integrated system permits high sensitivity and reproducible results.

Northern blot analysis of gene transcription is dependent on such factors as isolating intact, high-quality RNA, probe-labeling to high specific activity, removing unincorporated radiolabeled nucleotides, and hybridizing with conditions that maximize the signal-to-noise ratio. Products developed at Stratagene eliminate many variables that lead to poor quality Northern blot results. Consequently, we used these products for a Northern blot analysis of MSH2 gene transcription in a hamster cell model system.

MSH2 is important for DNA mismatch repair, and mutations in this gene are responsible for many cases of hereditary nonpolyposis colorectal cancer.1 The MSH2 gene encodes a 3.1-kb mRNA that is expressed at low to moderate levels.2 The larger-than-average size and
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Tools for Proteomic Science II
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. New Tools for Small RNA Analysis
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
8. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
9. Detecting miRNA & siRNA
10. Detecting Attomole Amounts of Small RNA
11. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tools for Detecting MSH2 Expression Chinese Hamster Ovary Cells

(Date:4/24/2015)... 24, 2015  Navitas Life Sciences is delighted to announce ... Clinical and Regulatory Services. Shalabh is a proven leader in ... CEO of Kinapse, as well as a Strategy consultant at ... - http://photos.prnewswire.com/prnh/20150423/201261LOGO ... "I am delighted to welcome Shalabh into this critical role ...
(Date:4/23/2015)... 2015 Athena San Diego, an organization ... women in San Diego’s technology and life science sectors, ... Awards at their Gala on April 30, 2015 from ... at http://athenasd.org/events/ , The Pinnacle Awards recognize ... Diego vision of fostering networking, risk taking and diversity ...
(Date:4/23/2015)... Washington, D.C. (PRWEB) April 23, 2015 ... Washington, D.C. region on April 4-17 as part ... visit marks the fourth group of biotechnology startups ... University of Nizhny Novgorod (UNN) and the University ... to deepen ties between their respective universities in ...
(Date:4/23/2015)... 2015  Cryoport, Inc. (OTCBB: CYRX) ("Company"), the ... the life sciences industry, today announced that the ... within the animal husbandry market by signing a ... the shipment of equine semen for artificial insemination ... Animal husbandry is a multi-billion dollar ...
Breaking Biology Technology:Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2Athena Pinnacle Awards to Honor Executives, Corporations and Educators Working to Promote Women Leaders 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 2U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 3U.S.-Russian Innovation Corridor Program Connects Visiting Russian Startups to U.S. Experts 4Cryoport to Support the Equine Animal Husbandry Market Through Agreement with Yancey Farms 2Cryoport to Support the Equine Animal Husbandry Market Through Agreement with Yancey Farms 3
... Call Scheduled for October 31, 2008 at 11:00 am ... plc,(Nasdaq: TRIB ), a leading developer and manufacturer ... will report,financial results for the third quarter of fiscal ... scheduled a conference call for that same,day, Friday, October ...
... Oct. 23 WaferGen Biosystems,Inc. (OTC Bulletin Board: ... cell biology and stem cell research systems,today announced ... will deliver a corporate presentation at the Seventh,Annual ... October 28-31, 2008,at the Palace Hotel in San ...
... SuperGen, Inc.,(Nasdaq: SUPG ), a ... of novel cancer therapies, today presented data ... additional posters, at the 20th,EORTC-NCI-AACR Symposium on ... MP-470, an orally bio-available multi-targeted,tyrosine kinase inhibitor, ...
Cached Biology Technology:Trinity Biotech plc to Announce Third Quarter 2008 Financial Results on October 31, 2008 2WaferGen to Present at the Seventh Annual BIO Investor Forum 2SuperGen's MP-470 Demonstrates Clinical Tumor Regression When Combined with Standard of Care Chemotherapy 2SuperGen's MP-470 Demonstrates Clinical Tumor Regression When Combined with Standard of Care Chemotherapy 3SuperGen's MP-470 Demonstrates Clinical Tumor Regression When Combined with Standard of Care Chemotherapy 4
(Date:4/2/2015)... , April 2, 2015 At its ... Salt Lake City , the American College of ... five new directors to its Board.  Members of the ... ACMG and for forming and advancing its policies and ... genetics profession. "It,s an eventful time in ...
(Date:4/1/2015)... 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... the growing mobile commerce market, announces the second shipment ... underway to early access pre-order customers. ... retail outlets including Walmart, Target, AT&T, Dunkin, Donuts, Stop ... accepted at all outlets and very easy to use. ...
(Date:3/31/2015)... March 31, 2015   Guidepoint , a leading ... the Post-Surgical Pain Management TRACKER, part of the ... trends in the medical device and therapeutics markets. The ... including treatment volumes, market share, and adoption rates across ... with joint surgery. The Post-Surgical Pain Management ...
Breaking Biology News(10 mins):American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3
... development, it can regenerate itself to such an extent ... of the heart cells remain healthy. Dr. Jrg-Detlef Drenckhahn ... Berlin-Buch made this discovery together with colleagues from Australia. ... the healthy cells of the heart divide more frequently ...
... on other planets and in extreme environments on Earth ... initiative under a five-year grant from NASA,s Astrobiology Institute ... Christopher H. House, associate professor of geosciences, will lead ... characterize life, look for biological signatures in relevant ecosystems ...
... stick to surfaces and walk up walls has inspired many ... a gecko,s foot. The secret behind the gecko,s ability to ... micro-/nano-scale on the underside of the gecko,s foot. Because there ... tightly to the surface the gecko is walking on by ...
Cached Biology News:Repair in the developing heart 2Penn State receives new NASA astrobiology grant 2Penn State receives new NASA astrobiology grant 3
...
... APAgene kits are designed to rapidly ... using our patented APA Technology. ... at an extraordinarily competitive price. All ... kit. The Locator Kit can be ...
Protein YIPF6 (YIP1 family member 6). [Source:Uniprot/SWISSPROT;Acc:Q96EC8] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
... to deparaffinize and perform Heat Induced Antigen ... sections mounted on glass microscope slides. Deparffinization ... the high temperature used in HIER (over ... paraffin by detergent. Formaldehyde fixation impairs or ...
Biology Products: