Navigation Links
Structural Determination of Flavonoids Using MSn

nt of the m/z 271 ion. The spectrum (Figure 6) is an exact match of the spectrum generated by the MS/MS experiment of apiginin (Figure 7). Compound X is a flavonoid! In fact, it is the flavonoid rhoifolin (apiginin 7-0-neohesperidoside).


Figure 7. Comparison of full-scan MS/MS (271) spectrum of apiginin with the full-scan MS4 (579>433>271>) mass spectrum of Compound X.

Conclusions

Electrospray ionization of an unknown compound provides a soft ionization technique allowing the generation of an intact pseudomolecular ion at m/z 579. The MS2 experiment yielded two major ions which were indicative of the loss of a deoxyhexose sugar (m/z 433) and a product mass which was the same as a base component of flavonoids (m/z 271). MS3 of the m/z 433 ion proved that the m/z 271 ion was a product of the m/z 433 ion and also indicated the loss of a hexose sugar. The MS4 experiment on the m/z 271 ion resulted in a fragmentation spectrum which was a direct match with the fragment spectrum of apiginin. The direct match indicates that the unknown compound belongs to the flavonoid class.





'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. Total Cellular Protein Determination Using the DC Protein Assay
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Structural Determination Flavonoids Using MSn

(Date:1/22/2015)... 22, 2015 Pipette.com has added the ... their comprehensive portfolio of Eppendorf products. , The Eppendorf ... a Centrifuge 5424/5424 R and receive the following:, ... Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... Diagenode, Inc., a leading global provider ... complete solutions for epigenetics research, recently launched a ... need for manual processing. The new ChIPettor System ... histones or transcription factors and a semi-automated pipette ...
(Date:1/22/2015)...  Derma Sciences, Inc. (Nasdaq: DSCI ), ... care, announces that AMNIOEXCEL® and AMNIOMATRIX®, amniotic allografts ... the Premier, Inc. Regenerative Skin Grafting contract portfolio. ... and AMNIOMATRIX® product lines, which continue to be ...
(Date:12/24/2014)... , Dec. 24, 2014   BioLife Solutions , Inc. (NASDAQ: ... of proprietary clinical grade hypothermic storage and ... for cells and tissues  ("BioLife" or the "Company"), today ... Stockholders on May 4, 2015 (the "Annual Meeting"). ...
Breaking Biology Technology:Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5BioLife Solutions Sets Date for Annual Meeting of Stockholders 2
... ... ... - Interaction of nimotuzumab with EGFRvIII, a mutant form of EGF receptor frequently ... , , ...
... LONDON and ... Matrix,Corporate Capital ("Matrix") and PharmaVentures Limited ("PharmaVentures"),today ... ("MOU") covering,strategic transactions in the biotech, pharmaceutical and ... identify licensing, buyout, investment,IPO and consolidation opportunities which ...
... Results from a landmark study involving more than 9,000 people showed that the high blood pressure medicine valsartan delayed progression to type 2 diabetes in patients ... ... , ... ...
Cached Biology Technology:YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 2YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 3YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 4YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 5YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 6YM BioSciences announces AACR acceptance of two nimotuzumab poster presentations 7Matrix Corporate Capital and PharmaVentures Announce the Signing of a Memorandum of Understanding (MOU) 2Matrix Corporate Capital and PharmaVentures Announce the Signing of a Memorandum of Understanding (MOU) 3Matrix Corporate Capital and PharmaVentures Announce the Signing of a Memorandum of Understanding (MOU) 4NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 2NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 3NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 4NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 5NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 6NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 7NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 8NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 9NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 10NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 11NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 12NAVIGATOR Shows Valsartan Delayed Progression to Type 2 Diabetes in At-Risk Cardiovascular Patients With Impaired Glucose Tolerance 13
(Date:1/22/2015)... Valid S.A. ("Company") (BM&FBOVESPA: VLID3 ON), is pleased to inform ... State of Washington,s Department of Licensing, in the ... issuance system for driver,s licenses and identification cards to its ... development will start in January 2015, with enrollment and card ...
(Date:1/22/2015)... , Jan. 15, 2015  BellBrook Labs, a ... discovery, announced the launch of a TR-FRET (time ... company,s Transcreener UDP Assay, a high throughput screening ... new assay will allow for sensitive detection of ...
(Date:1/22/2015)... 21, 2015  Analyst Report Issued by Small Cap IR. In ... that planet-wide, transactions at merchants on the leading payment cards ... of credit, debit, and prepaid cards reached 6.54 billion.  Eight ... fraudulent card usage in 2012, and credit and debit card ...
Breaking Biology News(10 mins):Valid USA Signs Contract For Washington Driver's License Issuance 2BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... LAFAYETTE, Ind. - Warmer, drier summers and extreme weather ... especially troublesome - possibly fatal - for walnut trees, ... years, Douglass Jacobs, a professor of forestry and natural ... Jacobs who is now a research scientist in the ...
... effects of climate warming on biodiversity pose a serious conservation ... Science .,Marine life may need to relocate faster than ... timing of major life cycle events. This challenges previous thinking ... than species on land because of slower warming in the ...
... of Cambridge study, which set out to investigate DNA methylation ... lifestyle and our health, has now mapped the link in ... collected greatly benefits a field that is still in its ... the researchers were, even 18 months ago. Researcher ...
Cached Biology News:Walnut trees may not be able to withstand climate change 2No plain sailing for marine life as climate warms 2No plain sailing for marine life as climate warms 3Environment and diet leave their prints on the heart 2
... Compliant software for JASCO HPLC systems. , Integrated Instrument Control ... EZChrom Elite ... for JASCO HPLC systems. This provides a completely integrated system to ... with each run. , ...
Rabbit anti Xanthine Oxidase Xanthine Oxidase, Bovine Buttermilk...
... SilverSNAP Stain for Mass Spectrometry Applications ... spectrometry (MS) has emerged as a powerful ... of 2-D gels is now an important ... that leads ultimately to identification of specific ...
... Protein Extraction Reagent is a ... that means no scraping, no ... With M-PER Reagent, rapid lysis ... at room temperature, yielding 25% ...
Biology Products: