Navigation Links
Structural Determination of Flavonoids Using MSn

may remove glycosylated side chains in a controlled, step-by-step manner using the power of MSn. Eventually, a core structure is exposed. A final fragmentation spectrum of the core structure yields a classic fingerprint which may then be referenced against a library MS/MS spectrum of the base component. In this way, a compound may be unequivocally classified.

Goal

This experiment demonstrates:
The utility of MSn for structural determination and unambiguous classification of a suspected flavonoid compound.
The utility of MSn for the unambiguous determination of product origin.

Experimental Conditions

Thermo Finnigan LCQ fitted with ESI probe
Infusion: 3 L/min using integrated syringe pump
Capillary temp: 150 C
Needle voltage: +4.2 kV
Sheath gas: 45 units
Auxiliary gas flow: 10 units

Results

Apiginin (Figure 1) (mw 270.2) is a base component of a class of flavonoids. The MS/MS spectrum of apiginin is rich in detail (Figure 2). Because the
LCQ imparts energy solely onto the precursor molecule, variations in collision energy generally have very little impact on the relative ratios of the product ions. The MS/MS spectrum of apiginin will be used as our library spectrum or fingerprint for the classification of Compound X whose full-scan mass spectrum is shown in Figure 3.


Figure 1. Structure and full-scan mass spectrum of apiginin.



Figure 2. Full-scan MS/MS (m/z 271) spectrum of apiginin.



Figure 3. Full
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. Total Cellular Protein Determination Using the DC Protein Assay
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Structural Determination Flavonoids Using MSn

(Date:11/26/2014)... 26, 2014 On January, 14, 2015, ... and Washington D.C. science community to the UMBC campus ... sponsored by the Protein Society, will take place from ... Room 312. Through discussion, presentations and workshops, the ... of proteins, their role in the industry and the ...
(Date:11/26/2014)... Silicon Valley Electronic Data Capture (EDC) ... Alliance , provider of clinical research and data management ... addition to its CRO Partnership Program, Clinovo expands ... India. , “We have found Clinovo’s ClinCapture to ... platform which is both user friendly and easy to ...
(Date:11/26/2014)... Boston, MA (PRWEB) November 26, 2014 ... and collaboration company is proud to announce that Darren ... Development and Sales. Darren will drive the ... and positive customer experience. , Darren brings ... He has held key roles in channel operations, from ...
(Date:11/26/2014)... /PRNewswrie/ -- Theravalues Corporation annuncia con orgoglio il lancio ... di Hi Europe 2014 (dal 2 al 4 dicembre, ... della curcumina con la maggiore biodisponibilità di sempre, con ... approvati dalle norme europee. La curcumina è ... ( Curcuma longa ) che è stata associata con ...
Breaking Biology Technology:RPM Alliance Joins Clinovo’s CRO Partnership Program, Further Strengthening Its Presence in Southern California and India 2SoundConnect Appoints Director of Channel Development & Sales 2La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 2La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 3
... venture capitalist in San Francisco or Boston, and youre on the ... Wisconsin. Everything looks right: The management team is in place, the ... ,But then a lawyer steps into the room, clears her ... lien law. Better not write that check, she says, until you ...
... state government information technology consolidation projects bound to be ... for the State of Wisconsin, has indicated the problems ... for large state projects. He has plenty of support ... are cost overruns, implementation delays, and outright failures really ...
... Madison, Wis. - GenTel BioSciences, Inc., ... $1.2 million Phase II SBIR grant from the National ... less painful diagnosis of respiratory allergies. , ,An estimated ... allergies, and current diagnostic tests are uncomfortable, especially for ...
Cached Biology Technology:Why repealing the shareholder wage lien law makes sense 2Why repealing the shareholder wage lien law makes sense 3State IT failures are not inevitable 2State IT failures are not inevitable 3State IT failures are not inevitable 4State IT failures are not inevitable 5GenTel gets federal grant to develop allergy test 2GenTel gets federal grant to develop allergy test 3
(Date:11/6/2014)... of metastasis, yet progress in preventing and treating ... been particularly challenging to design drugs that work ... systems biology at Harvard Medical School. , ... already metastasized." , Gujral and colleagues have now ... better understand how metastasis begins. Their findings may ...
(Date:11/5/2014)... has dedicated his career to understanding the Earth,s ... audience. , As deputy director and regional climatologist ... Desert Research Institute, Redmond has more than three ... dissemination of climate data to the general public. ... San Francisco on December 15-19, 2014 the American ...
(Date:11/4/2014)... Temple University School of Medicine announced today that ... initiative funded by the Bill & Melinda Gates ... Obstetrics, Gynecology and Reproductive Sciences; and Vice Chair ... School of Medicine will pursue an innovative global ... Biomarkers to Assess Fetal Neurodevelopment.", Grand Challenges Explorations ...
Breaking Biology News(10 mins):Migration negation 2Migration negation 3Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2Temple University School of Medicine receives Grand Challenges Explorations grant 2
... almost every plant species on the Iberian Peninsula had ... and Taraxacum lacianense , two dandelions from ... This finding confirms Spain,s privileged position as a hotbed ... now in Spain. It depends on the complexity of ...
... - William T. Talman, MD, President of the Federation ... Barack Obama for his continuing support for science and ... on research," Talman stated. In a letter to ... critical investmenteven during a time of unprecedented budgetary constraintswill ...
... More than a third of coral reef fish species ... climate change on coral reefs, a new scientific study has ... from individual locations, while they continue to persist elsewhere across ... international team of marine scientists has found that a third ...
Cached Biology News:2 new plants discovered in Spain 2Extinction predictor 'will help protect coral reefs' 2Extinction predictor 'will help protect coral reefs' 3
...
Buckets for tube holder inserts...
45° Fixed Angle Rotor for use in the IEC Centra CL2, Centra CL3/CL3R, HN-SII, and the Centra-MP4/MP4R....
... a microprocessor controlled, high speed filter ... exceptionally smooth operation make the Lambda ... fluorescence microscopy, ratio imaging, spectrophotometry, visual ... and accurate aperture positioning. The Lambda ...
Biology Products: