Navigation Links
Structural Determination of Flavonoids Using MSn

may remove glycosylated side chains in a controlled, step-by-step manner using the power of MSn. Eventually, a core structure is exposed. A final fragmentation spectrum of the core structure yields a classic fingerprint which may then be referenced against a library MS/MS spectrum of the base component. In this way, a compound may be unequivocally classified.

Goal

This experiment demonstrates:
The utility of MSn for structural determination and unambiguous classification of a suspected flavonoid compound.
The utility of MSn for the unambiguous determination of product origin.

Experimental Conditions

Thermo Finnigan LCQ fitted with ESI probe
Infusion: 3 L/min using integrated syringe pump
Capillary temp: 150 C
Needle voltage: +4.2 kV
Sheath gas: 45 units
Auxiliary gas flow: 10 units

Results

Apiginin (Figure 1) (mw 270.2) is a base component of a class of flavonoids. The MS/MS spectrum of apiginin is rich in detail (Figure 2). Because the
LCQ imparts energy solely onto the precursor molecule, variations in collision energy generally have very little impact on the relative ratios of the product ions. The MS/MS spectrum of apiginin will be used as our library spectrum or fingerprint for the classification of Compound X whose full-scan mass spectrum is shown in Figure 3.


Figure 1. Structure and full-scan mass spectrum of apiginin.



Figure 2. Full-scan MS/MS (m/z 271) spectrum of apiginin.



Figure 3. Full
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. Total Cellular Protein Determination Using the DC Protein Assay
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Structural Determination Flavonoids Using MSn

(Date:7/30/2015)... , July 30, 2015 Ascendis Pharma ... that applies its innovative TransCon technology to address ... results from a six-month Phase 2 study to ... Growth Hormone in 53 treatment-naïve, pre-pubertal children with ... are extremely pleased with the top-line results from ...
(Date:7/30/2015)... 30, 2015  Discovery Laboratories, Inc. (Nasdaq: ... the second $1.0M tranche under a previously awarded ... valued at up to $3.0 million to support ... as a potential medical countermeasure to mitigate acute ... awarded an initial $1.0 million under this grant ...
(Date:7/30/2015)... ... 30, 2015 , ... GEA's Pony™ NS2006L is ... up to 1500 bar. The sanitary design gauge of the NS2006L is well ... The Pony™ NS2006L homogenizer is an ideal solution for the processing of ...
(Date:7/29/2015)... , July 30, 2015 ... santé, publie ses résultats pour le premier semestre ... commente les résultats. Visionner l,interview vidéo ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Au sommaire de l,interview ... - Moteurs de croissance - Diabète ...
Breaking Biology Technology:Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2
... (Nasdaq: NXXI ), a developer and marketer ... levels, improve cardiovascular health, enhance memory and address chronic ... it redeemed all of its Series I outstanding Convertible ... shares. The Company redeemed 3,594 shares for a total ...
... 5:00 p.m. EDT - SOUTH SAN FRANCISCO, Calif., ... SNSS ) today reported financial results for the fourth ... for the year ended December 31, 2008 was $5.4 ... of December 31, 2008, cash, cash equivalents and marketable ...
... on probiotic microorganismsActive ingredient easily integrated into consumer products ... Association of Dental Research annual conference in Miami ... Germany and MIAMI, April 1 With an eye ... affecting an estimated 5 billion people world-wide, BASF -- ...
Cached Biology Technology:Nutrition 21 Redeems Series I Convertible Preferred Shares 2Nutrition 21 Redeems Series I Convertible Preferred Shares 3Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 2Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 3Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 4Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 5Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 6Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 7Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 8Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 9Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 10Sunesis Pharmaceuticals Reports Fourth Quarter and Full-Year 2008 Financial Results 11BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 2BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 3BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 4BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 5
(Date:7/31/2015)... La 10 th International Conference on Genomics ... 22 al 25 de octubre de 2015 en ... conferencia celebra su décimo aniversario. Desde su inauguración en el ... reuniones anuales más influyentes del mundo en el campo ,ómico, ... y mejores a nivel científico. ICG-10 se ...
(Date:7/31/2015)... -- The 10 th International Conference on Genomics (ICG-10, ... 2015, in Shenzhen , China ... th anniversary this year. Since its inauguration in 2006, ... meetings in the ,omics, fields, and is one of the ... ICG-10 focuses on recent breakthroughs and advances in ,omics, research ...
(Date:7/27/2015)... LOS ANGELES , July 27, 2015 /PRNewswire/ ... providing evidence- and experience-based clinical improvement solutions, today ... coordination solution is now available on Android smartphones ... at inpatient and post-care organizations can use ZynxCarebook ... the same patient,s condition, streamline care transitions to ...
Breaking Biology News(10 mins):La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3The 10th International Conference on Genomics (ICG-10) to Open in October 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 3
... with more corals and fish generate predictably greater levels ... This has important implications for understanding the behaviour of ... monitoring environmental health by listening to reefs. Contrary ... noisy places, with fish and invertebrates producing clicks and ...
... their abilities to counsel patients on a healthy diet and ... to a study by the University of Michigan Cardiovascular Center. ... time, being overweight, and if the doctor had adequate training ... study, published online ahead of print Oct. 1 in ...
... (September 21, 2010) For decades scientists believed ... contact with Homo sapiens , but new ... shows these sturdy ancients could adapt, innovate and ... by anthropologist Julien Riel-Salvatore challenge a half-century of ...
Cached Biology News:Taking the pulse of coral reefs 2Doctor's health habits affect patient counseling 2Neanderthals more advanced than previously thought 2
... The Biomek FX P is ... an,up-to-date hardware design for greater positional accuracy and ... just about any application. It can be configured ... its large deck capacity, the Biomek FX P ...
BD Falcon, microtest ELISA plate 96 well flat-bottom, clear. Polystyrene, nonsterile, no lid (25/sp, 100/ca) Packaging: 25 / pack, 100 / case ...
... GenomeLab GeXP Genetic Analysis System provides high-throughput, ... PCR*. The systems patented priming strategy ... PCR analysis to just a few genes ... more genes per reaction and more samples ...
... electrophoresis hood is a component ... Placed on the transilluminator, it ... and provides a darkroom environment ... lab bench. This 13 x ...
Biology Products: