Navigation Links
Structural Determination of Flavonoids Using MSn

Jack Cunniff, Philip Tiller, Michael Harvey, and Adrian Land,
Thermo Finnigan

Natural Product Analysis

The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers.

Introduction

For more than five thousand years tea has been used in China as an herbal remedy. In recent years pharmacological studies have supported some of these health claims. Flavonoids, a class of polyphenolic compounds, found in tea, plants and many fruits have shown some promising results related to anti-cancer as well as antiinflammatory and anti-allergy properties. For these reasons, there has been renewed interest in both detecting and characterizing these compounds.

In many instances, flavonoids exist as their glycosylated conjugate. In a typical MS/MS experiment, glycosylated moieties may be cleaved from the molecular precursor with a resulting MS/MS spectrum which is difficult to relate back to a hypothetical structure. The primary reason for the difficulty is the fact that first generation fragment ions may undergo further fragmentation, which can result in a very complex spectrum. Third and fourth generation product ions can not readily be distinguished from second generation product ions.

The LCQ Series of mass spectrometers can perform MSn, so precursor ions are isolated before a subsequent MS/MS experiment is performed. Due to this isolation step, fragmentation spectra are generally less complex and ambiguous than traditional MS/MS spectra. Through successive MS/MS steps, product origins can be assigned. As the following experiments will show, the LCQ
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. Total Cellular Protein Determination Using the DC Protein Assay
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Structural Determination Flavonoids Using MSn

(Date:10/20/2014)... MENLO PARK, Calif. , Oct. 20, 2014 ... announced today that the Company has signed a ... Institute of Regenerative Medicine (CIRM), effective October 1, ... of Clinical Development payments and the release of ... $14.3 million CIRM grant award for clinical development ...
(Date:10/20/2014)... (PRWEB) October 20, 2014 ... Sidney Kimmel Cancer Center at Thomas Jefferson University ... , Through the Strategic Alliance Partnership program, the ... will collaborate to raise awareness of the Center’s ... and other projects. Clinicians and other health care ...
(Date:10/19/2014)... Mass. , Oct. 19, 2014 NextCODE Health, ... the whole genome in real time, today announced the launch ... NextCODE Exchange , at the American Society of Human ... . To learn more, sign up and apply for free ... its unique features and benefits can be viewed here ...
(Date:10/19/2014)... 2014 The Asian Orthopedic braces and support ... with analysis and forecast of revenue. The Orthopedic braces and ... around $416.5 million by 2018, at a developing CAGR of ... of the Asian Orthopedic braces and support systems market, to ... provides a glimpse of the segmentation of orthopedic braces and ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4
... One of the more hopeful promises of stem cells is ... that is not being met by organ donation. But experts expressed ... lead to backlash: among patients if cures do not materialize, and ... transplants are just one way that stem cells could be used ...
... like a man on the hot seat, at least not ... manage the State of Wisconsin's information technology consolidation gave a ... of a state audit, during a "Consolidation for Optimization" program ... Despite criticism of the results, Miszewski said the challenges facing ...
... the core of such major product suites as Microsoft's .NET, ... However, the very openness of SOA can disrupt the internal ... Oxley Act (SOX), the 2002 federal law that was enacted ... , ,A typical internal control over financial reporting for ...
Cached Biology Technology:Stem cells raise hopes, but is there money in them? 2Stem cells raise hopes, but is there money in them? 3Miszewski touts benefits of IT integration 2Miszewski touts benefits of IT integration 3Managing SOX in the age of SOA 2Managing SOX in the age of SOA 3Managing SOX in the age of SOA 4
(Date:10/15/2014)... Lithuania , Oct. 15, 2014   ... technologies, today announced the availability of the ... VeriLook Surveillance 3.0 provides real-time biometric face identification ... high-resolution digital surveillance cameras. The new version not ... and differentiates people from objects while they are ...
(Date:10/14/2014)... Research shows SIRT6—a protein known to inhibit the ... of skin cancers by turning on an enzyme that ... , Previously considered protective, SIRT6 is part of a ... genomic stability and prevent some of the genetic flaws ... can lead to cancer. This study, in the journal,s ...
(Date:10/14/2014)... N.Y. – Scientists have sequenced the house fly genome ... one might expect from an insect that thrives in ... published Oct. 14 in the journal Genome Biology ... biology and of how flies quickly adapt to resist ... Adult house flies (Musca domestica) carry and transmit more ...
Breaking Biology News(10 mins):VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2House fly genome reveals expanded immune system 2
... the brain, cells called neurons go through a cycle ... Fernando Nottebohm, Ph.D., shows that these replaceable neurons share ... people with diseases such as Alzheimer's and Parkinson's: both ... , "It would be ironic if the shortfall in ...
... into the brain, researchers have treated a naturally occurring ... disease affecting the central nervous system to be successfully ... If this approach can be successfully applied to humans, ... entire class of diseases called lysosomal storage disorders which ...
... marine sponge is inspiring cutting-edge research in the design ... Barbara. , A report about these exciting new results ... story of the current issue of the scientific journal, ... Morse, professor of molecular, cellular and developmental biology at ...
Cached Biology News:One gene links newborn neurons with those that die in diseases such as Alzheimer's 2One gene links newborn neurons with those that die in diseases such as Alzheimer's 3One gene links newborn neurons with those that die in diseases such as Alzheimer's 4Genetic therapy reverses nervous system damage in animal model of inherited human disease 2Genetic therapy reverses nervous system damage in animal model of inherited human disease 3Marine sponge yields nanoscale secrets 2Marine sponge yields nanoscale secrets 3
Mouse monoclonal [CIG 3A11/5] to Cig2 (rating: *****) ( Abpromise for all tested applications)....
Rabbit polyclonal to CAMK 1D beta ( Abpromise for all tested applications). entrezGeneID: 57118 SwissProtID: Q5SQQ7...
Goat polyclonal to HNF4 ( Abpromise for all tested applications). Antigen: Synthetic peptide: RLSKTLVDMDMADY (Human) N terminal. Entrez GeneID: 3172 Swiss Protein ID: P41235...
IkappaB-alpha (112B2) Mouse mAb...
Biology Products: