Navigation Links
Structural Determination of Flavonoids Using MSn

Jack Cunniff, Philip Tiller, Michael Harvey, and Adrian Land,
Thermo Finnigan

Natural Product Analysis

The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers.

Introduction

For more than five thousand years tea has been used in China as an herbal remedy. In recent years pharmacological studies have supported some of these health claims. Flavonoids, a class of polyphenolic compounds, found in tea, plants and many fruits have shown some promising results related to anti-cancer as well as antiinflammatory and anti-allergy properties. For these reasons, there has been renewed interest in both detecting and characterizing these compounds.

In many instances, flavonoids exist as their glycosylated conjugate. In a typical MS/MS experiment, glycosylated moieties may be cleaved from the molecular precursor with a resulting MS/MS spectrum which is difficult to relate back to a hypothetical structure. The primary reason for the difficulty is the fact that first generation fragment ions may undergo further fragmentation, which can result in a very complex spectrum. Third and fourth generation product ions can not readily be distinguished from second generation product ions.

The LCQ Series of mass spectrometers can perform MSn, so precursor ions are isolated before a subsequent MS/MS experiment is performed. Due to this isolation step, fragmentation spectra are generally less complex and ambiguous than traditional MS/MS spectra. Through successive MS/MS steps, product origins can be assigned. As the following experiments will show, the LCQ
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. Total Cellular Protein Determination Using the DC Protein Assay
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
8. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
9. Determination of Acepromazine and its Major Metabolite in Equine Serum by LC-MS/MS using the Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
11. Oil-Free PCR Using the Hot Top Assembly
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Structural Determination Flavonoids Using MSn

(Date:9/2/2014)... Australia (PRWEB) September 02, 2014 The ... a cure for chronic tinnitus are seeking support for ... on the Indiegogo platform ( http://igg.me/at/tinaway ), was launched ... be a therapeutic ‘mHealth’ app and is based on ... of a team of biomedical and software engineers, Dr. ...
(Date:8/31/2014)... September 01, 2014 Available spectra ... needs for research and cultivation of a wide ... Valoya’s proprietary LEDs with proven patented and pending ... uncompromised light uniformity in critical applications in contrast ... LEDs. , “Our customers have a large variety ...
(Date:8/29/2014)... Electronic circuits are based on electrons, but one of ... photonic circuits, i.e. circuits based on light (photons) instead ... to create a stream of single photons and control ... sorts of attempts to achieve this control, but now ... creating a steady stream of photons emitted one at ...
(Date:8/29/2014)... Local veterinarian, Dr. Keith Clement, and his ... to participate in an investigational study of donor stem ... of this study is to determine if a single ... arthritically affected joints can help reduce pain and inflammation ... current investigational study must be older than nine months, ...
Breaking Biology Technology:tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 2tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 3tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 4tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 5Breakthrough in light sources for new quantum technology 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... Therapeutics S.A.,(Alternext: ALEHT), a drug and diagnostic discovery ... the annual shareholders, meeting of the Company, that ... de Vezin,s appointment as,member of the Supervisory Board, ... has reached the age limit defined in the ...
... 29 ,Anesiva, Inc. (Nasdaq: ANSV ) today ... with Green Vision Company, for,several Middle Eastern countries. ... approved in the U.S. to,provide topical local analgesia ... children three to 18 years of age., ...
... barrier technology from Singapore A*STARs Institute of Materials ... organic light emitting diodes (OLEDs) and solar cells ... other technology available in the market, opening up ... , A team of scientists from Singapores Institute ...
Cached Biology Technology:Frederic Desdouits Appointed Member of the Supervisory Board of ExonHit Therapeutics 2Frederic Desdouits Appointed Member of the Supervisory Board of ExonHit Therapeutics 3Anesiva Announces New Zingo Marketing and Distribution Agreement 2Anesiva Announces New Zingo Marketing and Distribution Agreement 3Nanoengineered barrier invented to protect plastic electronics from water degradation 2Nanoengineered barrier invented to protect plastic electronics from water degradation 3Nanoengineered barrier invented to protect plastic electronics from water degradation 4Nanoengineered barrier invented to protect plastic electronics from water degradation 5
(Date:8/31/2014)... is developing drugs that are highly effective against a ... patient. Such properties are directly related to the 3D ... have a shape that is perfectly complementary to a ... specificity. Publishing in Nature Chemistry , EPFL scientists ... the 3D structure of bioactive peptides and enhance their ...
(Date:8/31/2014)... is available in French . ... just published in the prestigious Nature Neuroscience journal ... Centre of the Douglas Mental Health University Institute and McGill ... circuitry and dynamic mechanisms controlling memory as well of the ... sub-region named the subiculum. , In 2009, they developed ...
(Date:8/29/2014)... how the repeated climatic shifts over the last ... genetic diversity, a team of researchers led by ... Carnaval developed a new biodiversity metric called "phylogeographic ... the genetic variation within species is restricted in ... of biology, and 14 other researchers from institutions ...
Breaking Biology News(10 mins):A new synthetic amino acid for an emerging class of drugs 2A new synthetic amino acid for an emerging class of drugs 3Memory and Alzheimer's: Towards a better comprehension of the dynamic mechanisms 2CCNY team defines new biodiversity metric 2
... published recently in PLoS One delivers new insight ... rattlesnakes may be engaged in an arms race with opossums, ... long been known to eat venomous snakes, this fact has ... of snake venom. Instead, snake venom is usually seen as ...
... see if you can spot the tallest tree. If you,re in ... there are few and far between, and tend to hunch low ... forests make the exercise a bit more difficult. And in the ... the eye can estimate. Knowing how tall trees can grow ...
... the University of Michigan Comprehensive Cancer Center have found that ... encouraging cancer stem cells to grow. Cancer stem cells ... drive its growth and spread. Traditional cancer treatments do not ... In a study published online in the Journal ...
Cached Biology News:Rapid venom evolution in pit vipers may be defensive 2Rapid venom evolution in pit vipers may be defensive 3MIT: The tallest tree in the land 2MIT: The tallest tree in the land 3Cancer stem cells recruit normal stem cells to fuel ovarian cancer, U-M study finds 2
... matrix for the extraction of unwanted ... other macromolecules, Applications: ,Reducing octyl glucoside ... interaction of pure β-adrenergic receptor and ... SDS in 6 M urea/0.05 mM ...
Values of steady state parameters for enzyme activities are determined....
... be used for the identification of potential biomarkers ... and other diseases. Due to the fact that ... it provides an attractive alternative to blood plasma ... ProteoSpin Urine Protein Concentration Micro Kit provides ...
... Antibody Capture Kit eliminates ... optimization steps typically required ... This kit enables the ... multiple antigens based on ...
Biology Products: