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Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

ent to 1.5- and 0.6-kb PCR products purified using the silica-gel membrane method (data not shown). For PCR products that were purified using the StrataPrep DNA gel extraction kit, the percentage of clones with inserts (based on blue-white color selection) was 97% for the 1.5-kb fragment and 95% for the 0.6-kb fragment. The corresponding percent of clones with inserts for PCR products that were purified using the silica-gel membrane was 83% for the 1.5-kb PCR product and 92% for the 0.6-kb PCR product.

Conclusions

The StrataPrep plasmid miniprep, PCR purification and DNA gel extraction kits are reliable, simple and convenient to use. Excellent yield of high-purity DNA with these kits facilitates digestion, sequencing, cloning and other manipulations of DNA.

REFERENCES

  1. Vogelstein, B., and Gillespie, D. (1979) Proc. Nat. Acad. Sci. USA 76: 615-619.

  2. Sambrook, J., Fritsch, E.F., and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.


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