Navigation Links
Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

figure 3

Sequence analysis of purified nucleic acids is a rigorous functional test of DNA purity. DNA purified with the StrataPrep kit was sequenced using fluorescent dye terminator and dye primer chemistries with an ABI PRISM 377 sequencer (figure 3). In this data set, only 3 sequence ambiguities were identified out of approximately 600 bases. In a separate sequencing experiment, plasmid DNA was sequenced using a LI-COR 4000L DNA sequencer and infrared-labeled sequencing primers. Sequence comparisons were made of plasmid DNA purified using either the StrataPrep kit or a silica-gel membrane method. Both sequences were aligned with a published data set. For the silica-gel membrane method, 12 ambiguous base calls were identified in 801 bases of sequence. Only 8 ambiguities were seen in 795 bases of sequence from plasmid that was purified using the StrataPrep plasmid miniprep kit (data available upon request).

Purification of PCR Products

Figure 4

The StrataPrep PCR purification kit is effective at removing primers and nonspecific amplification products smaller than 100 bp. Figure 4 shows that ethidium bromide-stained material smaller than 100 bp is eliminated from 0.6- and 1.5-kb PCR products using the StrataPrep kit (lanes 3 and 7, respectively), whereas the silica-gel membrane retained contaminants migrating between 72 and 118 bp (lanes 4 and 8, respectively).

figure 5

Cloning efficiency of the 1.5-kb PCR product purified using the StrataPrep ki
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
4. Simple Isolation of RNA from Tissue and Cultured Cells
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:2/26/2015)... --  Lexicon Pharmaceuticals, Inc. (Nasdaq: LXRX ) ... 35 th Annual Health Care Conference on Tuesday, March ... .  Lexicon management will provide an overview of Lexicon,s ... the presentation will be available through Lexicon,s website at ... available at www.lexpharma.com until April 3, 2015. ...
(Date:2/26/2015)... SAN FRANCISCO, Calif. , Feb. 26, 2015 /PRNewswire/ ... the treatment of cancer, today announced that Sean ... a corporate overview at the upcoming Cowen and Company ... Boston . Dr. McCarthy,s presentation will take ... About CytomX Therapeutics CytomX Therapeutics develops ...
(Date:2/26/2015)... Caris Life Sciences® today announced the ... Cancers Symposium in Orlando, Fla. ... profiling. Both studies utilized Caris Molecular Intelligence™, the ... biomarkers that shed light on potential treatment strategies ... cancer (SCBC). In one study, ...
(Date:2/25/2015)... Technologies Inc. (TSX-V: RTI) (the "Corporation") announced today that ... quarter of its fiscal year ended March 31, 2015. ... the CEO & CFO certifications for the three and ... on www.SEDAR.com . CFO ... pleased to announce the appointment of Prakash Hariharhan as ...
Breaking Biology Technology:Lexicon To Present At The Cowen 35th Annual Health Care Conference 2Tumor Profiling of Metastatic Prostate and Small Cell Bladder Cancers with Caris Molecular Intelligence Yields Insights into Novel and Conventional Therapeutic Options 2Tumor Profiling of Metastatic Prostate and Small Cell Bladder Cancers with Caris Molecular Intelligence Yields Insights into Novel and Conventional Therapeutic Options 3Tumor Profiling of Metastatic Prostate and Small Cell Bladder Cancers with Caris Molecular Intelligence Yields Insights into Novel and Conventional Therapeutic Options 4Tumor Profiling of Metastatic Prostate and Small Cell Bladder Cancers with Caris Molecular Intelligence Yields Insights into Novel and Conventional Therapeutic Options 5Radient Technologies Inc. Releases Third Quarter Results and Appoints New Interim CFO 2
... Calif., March 9 MannKind Corporation (Nasdaq: ... agreements with Pfizer Inc. (NYSE: PFE ) ... am Main, Germany and assets related to the production ... the production equipment, a quantity of bulk insulin and ...
... March 9 ExonHit Therapeutics (Alternext:,ALEHT) today announced that ... and redeemable November 8, 2011 have been converted,into shares. ... a EUR 17,231 capital increase and EUR,6,982,769 increase in ... debt is reduced to EUR 6,522,178 compared to EUR ...
... March 6 Finesse Solutions, LLC, Santa Clara, CA, ... sciences process applications, announced the technical demonstration of TruFluor(TM) ... Interphex in New York on March 17, 2009.The TruFluor ... of a disposable sheath, an optical reader, and a ...
Cached Biology Technology:MannKind Agrees with Pfizer on the Purchase of Frankfurt Insulin Manufacturing Plant 2MannKind Agrees with Pfizer on the Purchase of Frankfurt Insulin Manufacturing Plant 3MannKind Agrees with Pfizer on the Purchase of Frankfurt Insulin Manufacturing Plant 4ExonHit Therapeutics Shareholders' Equity Strengthened by Exercise of More Than Half of its Convertible Bonds 2Finesse Demonstrates TruFluor(TM) pH, a Single-Use Optical pH Sensor and Transmitter 2
(Date:2/24/2015)... Research and Markets ( http://www.researchandmarkets.com/research/sxwl43/global_2d_gesture ... "Global 2D Gesture Recognition Market 2015-2019" report ... forecast the Global 2D Gesture Recognition market to ... period 2014-2019 The increased demand for ... of the major trends in the market. The ...
(Date:2/13/2015)... , Feb. 13, 2015 ACT ... company that aims to transform cancer genomic information ... that the company has raised US$ 8 million in ... Based in Taipei, Taiwan , ... ACTDrug™, ACTOnco™. With the aim to implement next ...
(Date:2/10/2015)... 10, 2015  Alere Inc. (NYSE: ALR), a ... results for the quarter ended December 31, 2014. ... President of Alere said, "We made substantial progress ... the global leader in rapid diagnostics and delivering ... Health divestiture in early January enabled us to ...
Breaking Biology News(10 mins):Global 2D Gesture Recognition Market 2015-2019 with Cognitec Systems, CogniVue, eyeSight Mobile Technologies & PointGrab Dominating 2ACT Genomics Raises $8 Million in its First Private Funding Round 2Alere Inc. Announces Fourth Quarter 2014 Results 2Alere Inc. Announces Fourth Quarter 2014 Results 3Alere Inc. Announces Fourth Quarter 2014 Results 4Alere Inc. Announces Fourth Quarter 2014 Results 5Alere Inc. Announces Fourth Quarter 2014 Results 6Alere Inc. Announces Fourth Quarter 2014 Results 7Alere Inc. Announces Fourth Quarter 2014 Results 8Alere Inc. Announces Fourth Quarter 2014 Results 9Alere Inc. Announces Fourth Quarter 2014 Results 10Alere Inc. Announces Fourth Quarter 2014 Results 11Alere Inc. Announces Fourth Quarter 2014 Results 12Alere Inc. Announces Fourth Quarter 2014 Results 13Alere Inc. Announces Fourth Quarter 2014 Results 14Alere Inc. Announces Fourth Quarter 2014 Results 15Alere Inc. Announces Fourth Quarter 2014 Results 16Alere Inc. Announces Fourth Quarter 2014 Results 17Alere Inc. Announces Fourth Quarter 2014 Results 18Alere Inc. Announces Fourth Quarter 2014 Results 19Alere Inc. Announces Fourth Quarter 2014 Results 20Alere Inc. Announces Fourth Quarter 2014 Results 21Alere Inc. Announces Fourth Quarter 2014 Results 22Alere Inc. Announces Fourth Quarter 2014 Results 23Alere Inc. Announces Fourth Quarter 2014 Results 24Alere Inc. Announces Fourth Quarter 2014 Results 25Alere Inc. Announces Fourth Quarter 2014 Results 26Alere Inc. Announces Fourth Quarter 2014 Results 27Alere Inc. Announces Fourth Quarter 2014 Results 28Alere Inc. Announces Fourth Quarter 2014 Results 29Alere Inc. Announces Fourth Quarter 2014 Results 30Alere Inc. Announces Fourth Quarter 2014 Results 31Alere Inc. Announces Fourth Quarter 2014 Results 32Alere Inc. Announces Fourth Quarter 2014 Results 33Alere Inc. Announces Fourth Quarter 2014 Results 34
... 20 May 2012. The Stem Cells and Cancer Research ... Vall d,Hebrn Institute of Oncology (VHIO) has identified the ... used in clinical trials for colon cancer treatment. The ... with Professor Alberto Muozs laboratory at the Instituto de ...
... PHOENIX, May 18, 2012 /PRNewswire-iReach/ -- With ... out of sight, and research to find "cures" continuing ... remain "a few years away," a small but growing ... human consciousness. A number of healing practices that rely ...
... did not set out to discover one of the longest ... and a team of researchers all current or former ... published in Scientific Reports . Their findings shed ... lead to widespread, yet largely invisible, disruptions of ecological interaction ...
Cached Biology News:Discovery of mechanisms predicting response to new treatments in colon cancer 2Discovery of mechanisms predicting response to new treatments in colon cancer 3Healing Method from Australia Addresses the Human Genome 2Stanford scientists document fragile land-sea ecological chain 2
... Plant Leaf DNA Purification Kit is designed for ... leaf tissue. The protocol can be completed in ... up to obtain larger amounts of DNA. The ... restriction digestion, and Southern blotting. The MasterPure Kit ...
Caspase Activity Detector, SR-VAD-FMK Kit , 100 tests Consult technical datasheet for details....
HistoScan AP Universal Goat Kit...
...
Biology Products: