Navigation Links
Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

ash and spin
  • Add elution buffer and spin to collect DNA
  • Figure 1 outlines the simple and rapid protocols used for purifying miniprep plasmid DNA, PCR products and agarose gel-fractionated DNA. Each microspin cup in the StrataPrep plasmid miniprep kit yields up to 20 g of purified plasmid DNA from overnight bacterial cultures of 1.5 to 3 ml. Each microspin cup in the StrataPrep PCR purification kit and DNA gel extraction kit yields as much as 10 g of purified DNA.

    Plasmid Purification

    figure 2

    Plasmid DNA isolated with the StrataPrep plasmid miniprep kit exhibits OD260 -to-OD280 ratios between 1.8 and 2.0, indicating a high degree of nucleic acid purity.2 Starting with 30 individual 1.5- to 3-ml liquid cultures, purified plasmid DNA can be obtained in an average of 2.5 minutes per miniprep. Plasmid and cosmid DNAs isolated with the StrataPrep plasmid miniprep kit are easily digested with restriction enzymes (figure 2). Plasmid DNA purified with the StrataPrep kit transforms chemically competent E. coli with efficiencies equivalent to those obtained for DNA that has been purified using cesium chloride density gradients. For example, 100 pg of a 2.9-kb plasmid purified using either the StrataPrep kit or the cesium chloride density gradient method transformed Epicurian Coli XL2-Blue ultracompetent cells with an efficiency of 2 x 109 colony forming units (cfu)/g of plasmid DNA (data not shown).


    '"/>

    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
    2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
    3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
    4. Simple Isolation of RNA from Tissue and Cultured Cells
    5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
    6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
    7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
    8. Simple Approaches for Optimization of RT-PCR
    9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
    10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
    11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
    Post Your Comments:
    (Date:7/23/2014)... (PRWEB) July 23, 2014 ... innovative and comprehensive consultancy and pharma training services, ... and Safety (ACRES) , a non-profit integrating the ... build and maintain a global system for clinical ... collaboration to improve the expertise of industry professionals ...
    (Date:7/22/2014)... from the National Institute of Standards and Technology ... demonstrated a new design for an atomic clock ... or a microcomb. , The microcomb clock, featured ... the new journal Optica ,* is the ... and its accurate conversion of optical frequencies to ...
    (Date:7/22/2014)... New York (PRWEB) July 22, 2014 ... world. The heat produced by controlled fission chain reaction ... This steam pressure is used to drive a power- ... approximately 19% of the world’s total electricity production. Nuclear ... to several under construction, planned and proposed nuclear reactor ...
    (Date:7/22/2014)... (PRWEB) July 22, 2014 The ... clear and concise visual representation of history, migration, and ... was always regaled by family history stories stemming from ... as the "old country." Pictures and 8mm home movies ... side of the family, and Steve ordered a test ...
    Breaking Biology Technology:Astra Nova and ACRES Partner on Quality and Access of Training for Research Professionals 2Astra Nova and ACRES Partner on Quality and Access of Training for Research Professionals 3'Comb on a chip' powers new NIST/Caltech atomic clock design 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4
    ... executives, mostly in denial, are shunning the subject. Smart ones ... general broadcasts. This is a revolution, and the entire model ... , Device Explosion , ,The receptacles to receive the ... but rather handheld devices or the hundreds of gadgets and ...
    ... Read Part 1 . Winslow Sargeants combination of ... the world of entrepreneurship and risk. His memories of growing ... tenacity is essential. , , Winslow Sargeant ... a child. In June, 1974, U.S. District Court Judge W. ...
    ... MadCity Murder Mystery game Madison, Wis. ... the UW-Madison School of Education, hopes to bring a ... games, which combine aspects of physical activity, traditional reading ... through the UW System and the Academic ADL ...
    Cached Biology Technology:When TV surrenders to Web... 2When TV surrenders to Web... 3Winslow Sargeant: Stepping onto the train called Wisconsin - Part 2 2Winslow Sargeant: Stepping onto the train called Wisconsin - Part 2 3Winslow Sargeant: Stepping onto the train called Wisconsin - Part 2 4Augmented reality brings a new dimension to learning 2Augmented reality brings a new dimension to learning 3
    (Date:7/23/2014)... are disappearing, and though conservationists are examining several possible ... rain and light pollution, a key piece of the ... once they leave their breeding sites, and what threats ... answer this question, a team of researchers out of ... map produced for a songbird, using tracking from both ...
    (Date:7/23/2014)... 780 million people around the world have no access ... Organization (WHO), 3.4 million people die from water-related diseases ... goal of making a contribution to solving this problem. ... Stark, head of the Functional Materials Laboratory, the 23-year-old ... a prototype. , "What makes our DrinkPure filter unique ...
    (Date:7/23/2014)... This news release is available in German . ... shape and stability to cells, drive metabolic processes and ... into complex three-dimensional shapes. Scientists at the Max Planck ... that proteins can be constructed of similar amino acid ... suggests that the proteins that exist today arose from ...
    Breaking Biology News(10 mins):York University researchers use bird 'backpacks' to put wood thrushes migration on the map 2ETH student develops filter for clean water around the world 2Protein evolution follows a modular principle 2
    ... announced a new Center for Environmental Policy that ... environmental debate and offer practical solutions for managing ... funded by an $82,500 start-up grant from the ... of environmental policy affecting Philadelphia and surrounding communities ...
    ... the rage in major media headlines, but for the human ... researchers from Denmark and The Netherlands published in the October ... brain, just like muscles, works harder during strenuous exercise and ... this finding help explain why the brain is able to ...
    ... Steve Brusatte, Professor Michael Benton, and colleagues at ... proliferate immediately after they originated, but that their rise ... two mass extinctions. "The sheer size of ... special about these animals that preordained them for success ...
    Cached Biology News:New center will focus environmental debate and produce solutions for action 2New center will focus environmental debate and produce solutions for action 3Mass extinctions and the evolution of dinosaurs 2
    ... greater incorporation of Br-dUTP results in a ... using a fluorescein-labeled anti-BrdU antibody. Propidium ... kit to counterstain the total DNA. Features ... in improved detection than found by using ...
    ... cytoplasmic expression of an N-terminal FLAG® fusion ... selection marker from bicistronic mRNA. Two ... MCS2 for transcription of a single message ... useful for protein-protein interaction studies, multi-subunit proteins, ...
    ...
    Mouse monoclonal antibody raised against a partial recombinant SCARB2. NCBI Entrez Gene ID = SCARB2...
    Biology Products: