Navigation Links
Simple Isolation of RNA from Tissue and Cultured Cells


Easy RNA purification with integrated DNA-removal step

Karen Dolter Jeff Braman
Stratagene

The StrataPrep total RNA miniprep kit efficiently isolates high-quality total RNA from 10 5 to 10 7 cultured cells or 10 to 40 mg of tissue and includes an on-column DNA-removal step. Additionally, multiple samples can be processed simultaneously because of the easy-to-follow technique. The RNA isolation is performed within microspin cups, which eliminates the need for organic extraction or ethanol precipitation. The resulting total RNA is suitable for a wide variety of demanding molecular biology applications.

Fig.1

High-quality RNA is necessary for techniques such as cDNA synthesis, RT-PCR amplification, RNase protection assay, primer extension analysis, and Northern blotting. Stratagenes StrataPrep total RNA miniprep method takes advantage of the efficient denaturing properties of guanidine thiocyanate for cell lysis. The protein-denaturing ability of this chaotropic salt facilitates the isolation of intact RNA from tissue rich in ribonucleases. The method originally described1 has been simplified by using a silica-based fiber matrix in a microspin-cup format (Figure 1). The RNA is immobilized on the fiber matrix, allowing contaminant removal while avoiding organic extractions. In addition, no ethanol precipitation of the RNA is necessary. By treating the RNA sample directly on the fiber matrix with DNase, DNA contamination is reduced to undetectable levels. Once the purified RNA is eluted from the fiber matrix in a small volume of buffer or water, it is ready to use.

We showed that the kit is capable of high performance using
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Isolation RNA from Tissue and Cultured Cells

(Date:9/3/2015)... ... September 03, 2015 , ... Park Systems announced that they will ... at 9am PST. The webinar will focus on the methods and materials for additive ... additive manufacturing is a broad term that encompasses “addition or fusion” of materials to ...
(Date:9/2/2015)... ARBOR, Mich. and LIBERTYVILLE, Ill. (PRWEB) , ... ... ... NSF International, a leading provider of regulatory compliance, quality systems, analytical ... Avarent LLC, a consulting firm with expertise in medical device products/processes development, ...
(Date:9/2/2015)... 02, 2015 Research and ... Jain PharmaBiotech,s new report "Gene Therapy - Technologies, ... The markets for gene therapy are difficult to estimate ... and it is marketed in China ... the years 2014-2024. The estimates are based on epidemiology ...
(Date:9/2/2015)... , Sept. 2, 2015 Australian prostate cancer ... prestigious Australian science prize recognising ,outstanding, science that uses ... to globally launch a novel prostate cancer diagnostic test ... high level consortium taking out the peer-reviewed Australian Museum ... Research. This prize is awarded for ...
Breaking Biology Technology:Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 2Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 3Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 4NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 2NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 4NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 5NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 6NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 7Global Gene Therapy Market Study 2015-2024 - Technologies, Markets and Companies Analysis 2Prostate Cancer Company Minomic Wins Prestigious Eureka Science Prize 2Prostate Cancer Company Minomic Wins Prestigious Eureka Science Prize 3
... announced,today that it has sold T-COTE, its proprietary ... sale, Sensient assumes all marketing and,sales responsibilities and ... will remain involved during the transition for,technical support. ... to the product,its specifications or method and location ...
... Imaging,Diagnostic Systems, Inc., (OTC Bulletin Board: IMDS) ... announced today that its distributor,The Oyamo group has ... Mammography,System (CTLM(R)) for Jerusalem, Israel., (Logo: ... the import license from the Israel Ministry of,Health ...
... patients and caregivers from around the ... world ... GBS/CIDP Foundation,International will host its tenth international symposium on Guillain-Barre,Syndrome ... November 7-9, 2008. Over 250 medical professionals,current and former patients ...
Cached Biology Technology:Particle Sciences sells T-COTE(R) Line of Microfine Titanium Dioxides to Sensient 2Imaging Diagnostic Systems Receives its First Order for its CT Laser Mammography System for Israel 2GBS/CIDP Foundation to Hold 10th International Symposium 2
(Date:8/20/2015)... 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market and creator ... The Smart Card Alliance and the EMV Migration Forum.  ... the Smart Card Alliance Latin America (SCALA) Chapter provides ... and thought leaders promoting adoption of smart card technology. ...
(Date:8/17/2015)... , Aug. 17, 2015  NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market ... new sales campaign for Q4 2015. The new marketing ... team consisting of Amplitude Marketing Group ( http://amplitudemarketing.com/ ) ... Group is a leader in retail driven marketing and ...
(Date:8/12/2015)... SAN JOSE, Calif. , Aug. 12, 2015 ... leading developer of human interface solutions, today announced ... the recently announced Match-in-Sensor solution, have officially been ... (FIDO) Alliance. As part of the certification, Synaptics, ... the FIDO 1.0 Universal Authentication Framework (UAF) standard ...
Breaking Biology News(10 mins):NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3
... have charted the path of insulin action in cells in ... for understanding what goes wrong in diabetes. The ... from Sydney,s Garvan Institute of Medical Research, is now published ... Cell Metabolism . First discovered in 1921, the insulin ...
... (MAY 20, 2013) University of Minnesota Medical School ... in partnership with the University,s Brain Tumor Program, have ... sheath tumors (MPNST) that allow them to discover new ... The research was published this week in the ...
... A National Science Foundation (NSF) planning grant will help ... a joint program of the University of Illinois at ... "CARD will be devoted to research in drying ... products; forestry and paper products; chemical products; textiles; and ...
Cached Biology News:Insight into the dazzling impact of insulin in cells 2Insight into the dazzling impact of insulin in cells 3U of M researchers develop model for better testing, targeting of MPNST 2NSF approves planning grant for Center for Advanced Research in Drying 2