Navigation Links
Simple Isolation of RNA from Tissue and Cultured Cells


Easy RNA purification with integrated DNA-removal step

Karen Dolter Jeff Braman
Stratagene

The StrataPrep total RNA miniprep kit efficiently isolates high-quality total RNA from 10 5 to 10 7 cultured cells or 10 to 40 mg of tissue and includes an on-column DNA-removal step. Additionally, multiple samples can be processed simultaneously because of the easy-to-follow technique. The RNA isolation is performed within microspin cups, which eliminates the need for organic extraction or ethanol precipitation. The resulting total RNA is suitable for a wide variety of demanding molecular biology applications.

Fig.1

High-quality RNA is necessary for techniques such as cDNA synthesis, RT-PCR amplification, RNase protection assay, primer extension analysis, and Northern blotting. Stratagenes StrataPrep total RNA miniprep method takes advantage of the efficient denaturing properties of guanidine thiocyanate for cell lysis. The protein-denaturing ability of this chaotropic salt facilitates the isolation of intact RNA from tissue rich in ribonucleases. The method originally described1 has been simplified by using a silica-based fiber matrix in a microspin-cup format (Figure 1). The RNA is immobilized on the fiber matrix, allowing contaminant removal while avoiding organic extractions. In addition, no ethanol precipitation of the RNA is necessary. By treating the RNA sample directly on the fiber matrix with DNase, DNA contamination is reduced to undetectable levels. Once the purified RNA is eluted from the fiber matrix in a small volume of buffer or water, it is ready to use.

We showed that the kit is capable of high performance using
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Isolation RNA from Tissue and Cultured Cells

(Date:3/27/2015)... Working in collaboration with the National ... London , Richmond Pharmacology is the first centre worldwide ... for an investigational RNAi therapeutic being developed for the treatment ... and heart. Read press release ... at St Georges University of London , ...
(Date:3/26/2015)... 2015  Roka Bioscience, Inc. (NASDAQ: ROKA ... advanced testing solutions for the detection of foodborne ... three months and full year ended December 31, ... for the quarter ended December 31, 2014 was ... quarter of 2013 and $1.5 million for the ...
(Date:3/26/2015)... -- BioNano Genomics, Inc., the leader in genome mapping, announced ... commercial officer. Salyer has more than 20 years of ... sciences. At BioNano, Salyer will oversee the commercialization of ... assemble a comprehensive view of complex genomes to help ... located in the United States , ...
(Date:3/26/2015)... (PRWEB) March 26, 2015 ReliantHeart, Inc., ... and Transonic, a supplier of precision transit-time flow measurement ... will greatly improve the efficiency of the HeartAssist5® ... LVAD patients to experience greater mobility and peace of ... the miniature flow board, will draw 1/6th of the ...
Breaking Biology Technology:Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 2Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 3Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 4Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 5Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 6Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 7BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 3
... Note: I had the opportunity to speak with Congresswomen ... in bringing more federal dollars to Wisconsin for technology ... regionally-based economic development. , ,Although Baldwin is best known ... veterans, womens rights and the environment, she is emerging ...
... facility will be unlike any other in the Midwest. Only ... of them in the Midwest region, said Terrence R. Dolan, ... 35,000 square feet of laboratory space, the factory will be ... project reveal that there is a growing list of global ...
... Iyer, founder of Samyog Software Limited , discusses ... for companies contemplating offshore outsourcing. Samyog designs and delivers ... supply chain management and logistics. Their solutions may be ... tools. , , WTN: What is Samyog Softwares background? ...
Cached Biology Technology:WTN Interview with Congresswoman Tammy Baldwin: Facilitating technology growth and regional economic development is a priority 2WTN Interview with Congresswoman Tammy Baldwin: Facilitating technology growth and regional economic development is a priority 3WTN Interview with Congresswoman Tammy Baldwin: Facilitating technology growth and regional economic development is a priority 4WTN Interview with Congresswoman Tammy Baldwin: Facilitating technology growth and regional economic development is a priority 5WTN Interview with Congresswoman Tammy Baldwin: Facilitating technology growth and regional economic development is a priority 6Biotech Manufacturing Facility to be Built in Wisconsin 2Lessons in Offshore Software Development 2Lessons in Offshore Software Development 3Lessons in Offshore Software Development 4Lessons in Offshore Software Development 5
(Date:3/18/2015)... 2015 As mobile payments become more ... wallets and apps continue to be introduced into the market ... mobile payment industry in focus today are:  NXT-ID, Inc. (NASDAQ: ... BABA ), Apple Inc. (NASDAQ: AAPL ), ... (NASDAQ: FB ) NXT-ID, Inc. (NASDAQ: ...
(Date:3/12/2015)... March 12, 2015 IriTech, a leading iris ... of the Texas Instruments Design Network, announced today that ... demonstrated its IriShield USB MK2120U device during a launching ... pension distribution. The iris scanner is ... marketed in India through its ...
(Date:3/11/2015)... BOSTON , March 11, 2015   ... of its platform to scientifically measure and harness ... characteristics to real-time biometrics and objective measurements of ... of music at scale in large populations. It ... scientists, technologists and clinicians perform rigorous studies and ...
Breaking Biology News(10 mins):Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 2Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 4Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 6Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 2Introducing The Sync Project, a Global Collaboration Harnessing the Scientific Potential of Music for Health 3
... of climate change on the world,s oceans has found they ... million years. In an article published today in ... man-made greenhouse gases are driving irreversible and dramatic changes to ... hundreds of millions of people across the planet. The ...
... Argonne Leadership Computing Facility (ALCF), located at the U.S. ... over two billion processor-hours of computations at a mind-boggling ... it enables scientists and engineers to conduct cutting-edge research ... "The ALCF is dedicated to enabling breakthrough science ...
... ST. LOUIS In research led by a Saint Louis ... the enzyme thrombin,s anti-blood clotting properties. The finding opens the ... thrombosis, the presence of blood clots in blood vessels, which ... the U.S. "Thrombosis is one of the most ...
Cached Biology News:Ocean changes may have dire impact on people 2Ocean changes may have dire impact on people 3Over two billion hours served 2Saint Louis University investigators perfect new version of blood-regulator thrombin 2Saint Louis University investigators perfect new version of blood-regulator thrombin 3
... solutions to meet your laboratory's requirements for data management and quality control. ... ... ... CLARA® is the standard software for Staccato Systems and custom Allegro Modules allowing for the simple ...
This kit is designed to select cells labeled with phycoerythrin- (PE-) conjugated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
... DASGIP Overhead Drive for individual and parallel ... flexible agitation of DASGIP vessels, featuring range ... ,Up to 16 overhead drives can ... Cultivation Systems fedbatch-pro® and cellferm-pro® for microbiology ...
... Whatever your Ion Chromatography requirements, ... . The newly developed conductivity detector ... ultra-low drift . In addition to ... be used with other detectors, such as ...
Biology Products: