Navigation Links
Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins


Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Detection of expressed tags made easy

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang
Stratagene

Stratagenes TagDetect FLAG Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusion proteins. To visualize results, the kit uses a colloidal-gold (CG) labeled anti-FLAG M2 monoclonal antibody in a dipstick format. This highly specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, and it will not cross react with non-specific prokaryotic and eukaryotic proteins. It is sensitive and fast: Detect as little as 10 ng/ml of expressed FLAG-tagged fusion protein in just 10 minutes.

Epitope-tagging technology provides a powerful means to functionally analyze genes in eukaryotic cells. With this technique, a tag-specific antibody is used to target and analyze the fusion protein within the cell, without making a specific antibody to each new protein under study.1

Epitope tags are usually short polypeptides with defined sequences, which can be terminally or internally incorporated into the protein of interest. Sequences coding for common epitopes have been incorporated into a variety of cloning and expression vectors. Since the FLAG peptide (DYKDDDDK) is a commonly used epitope tag, Stratagene developed the TagDetect FLAG Protein Dipstick. It can be used to monitor expression of FLAG-tagged proteins in either prokaryotic or eukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps, and to identify proteins following immun
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
2. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
3. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
9. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
10. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
11. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive and Rapid Detection FLAG Tagged Fusion Proteins

(Date:3/27/2015)... , March 27, 2015  Peter Walter was ... Science for his pioneering work on how proteins are ... of a regulatory mechanism that cells use to handle ... Walter is a professor of biochemistry at the University ... for treating a range of human diseases related to ...
(Date:3/27/2015)... , March 27, 2015 WuXi ... leading open-access R&D capability and technology platform company ... industries, announced today that an Investigational New Drug ... antibody for rheumatoid arthritis has been accepted for ... (CFDA).  In September 2012, MedImmune, ...
(Date:3/27/2015)... New York (PRWEB) March 27, 2015 ... the 2015 application period for 43North - the world’s ... prizes, including a grand prize of $1 million, six ... entrepreneurs from around the world to compete for prizes ... funded enterprises in the Western New York region. ...
(Date:3/26/2015)... March 26, 2015 PRC Clinical, a ... today several management and executive appointments effective immediately. , ... Officer (CFO) and member of the Board of Directors. ... Operations, and Sue Dowden will assume the position of ... current Chief Operations Officer (COO), has been appointed to ...
Breaking Biology Technology:German-born cell biologist Peter Walter wins the 2015 Vilcek Prize in Biomedical Science 2German-born cell biologist Peter Walter wins the 2015 Vilcek Prize in Biomedical Science 3Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 2Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 3Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 2Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 3Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 4Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 5PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3
... ... Austin, Texas (PRWEB) April 29, 2009 -- Caringo Inc., ... distributing content, today announced the release of CAStor 3.0, featuring storage ... nodes in a single, physical server chassis. CAStor Cluster in a ...
... 29 Medarex, Inc. (Nasdaq: MEDX ) ... webcast at 8:30 a.m. Eastern Time on Thursday, April ... results for the first quarter of 2009.To access the ... The webcast can be accessed through the Investor Relations ...
... improves balance sheetROCKVILLE, Md., April 29 Novavax, Inc. ... development of novel vaccines, today announced that it has ... million of the total $22 million in outstanding convertible ... of 4.75% payable semi-annually and had a maturity date ...
Cached Biology Technology:Caringo Delivers Storage Cluster in a Box with CAStor 3.0 2Medarex to Host Business and Financial Update Conference Call on Thursday, April 30, 2009 2Novavax Announces Early Retirement of $17 Million of Convertible Debt 2Novavax Announces Early Retirement of $17 Million of Convertible Debt 3
(Date:3/18/2015)... Florida , March 18, 2015 ... as businesses, new revolutionary smart wallets and apps continue to ... Companies currently entrenched in the mobile payment industry in focus ... Group Holding Ltd. (NYSE: BABA ), Apple Inc. ... GOOG ) and Facebook Inc. (NASDAQ: FB ) ...
(Date:3/17/2015)... YORK , March 17, 2015  MecklerMedia ... the largest exhibition for service robots ever held ... RoboUniverse takes place on May 11-13, 2015 at ... and exhibitors for the event include: Acorn Product ... Control; EZ-Robot; Infranor; Kinova Robotics; Littler; NewBotic Corporation; ...
(Date:3/12/2015)... , March 12, 2015 The mascots of ... Children,s Hospital, part of Wake Forest Baptist Medical Center. ... for the 2015 ACC Men,s Basketball Tournament. Young patients ... them and getting their autographs. Brenner Children,s ... It is the only children,s hospital in northwest ...
Breaking Biology News(10 mins):Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 2Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 4Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 6MecklerMedia's RoboUniverse New York Announces Sponsors and Exhibitors, May 11-13, 2015 2
... Hikers know that moss on a tree trunk always points ... this ancient plant may also provide a new "compass" for ... cells for medical purposes. Dr. Nir Ohad ... Ralf Reski of the University of Freiburg have discovered a ...
... You can learn a lot from an animal. By ... have discovered a great deal about the genes and molecules ... work about the same in people as they do in ... cognitive processes such as humans, ability to learn language from ...
... main objective of the research was to gather information on ... the hydrological and dynamic characteristics of plankton in the estuaries. ... and his work entitled Impact of climate variability on the ... (Bay of Biscay): a time series analysis. He has studied ...
Cached Biology News:Telltale moss 2Transgenic songbirds provide new tool to understand the brain 2University of Basque Country research study on effects of climate on plankton in the estuaries 2
Cell Culture Flask, 25 cm, tissue-culture treated polystyrene...
Cell Culture Flask, nontreated polystyrene...
iNOS/NOS Type II Purified Anti-Mouse, Anti-Rat, Anti-Human clone Polyclonal, Isotype Rabbit Ig, 150 µg Consult technical datasheet for details....
...
Biology Products: