Navigation Links
Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins


Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Detection of expressed tags made easy

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang
Stratagene

Stratagenes TagDetect FLAG Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusion proteins. To visualize results, the kit uses a colloidal-gold (CG) labeled anti-FLAG M2 monoclonal antibody in a dipstick format. This highly specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, and it will not cross react with non-specific prokaryotic and eukaryotic proteins. It is sensitive and fast: Detect as little as 10 ng/ml of expressed FLAG-tagged fusion protein in just 10 minutes.

Epitope-tagging technology provides a powerful means to functionally analyze genes in eukaryotic cells. With this technique, a tag-specific antibody is used to target and analyze the fusion protein within the cell, without making a specific antibody to each new protein under study.1

Epitope tags are usually short polypeptides with defined sequences, which can be terminally or internally incorporated into the protein of interest. Sequences coding for common epitopes have been incorporated into a variety of cloning and expression vectors. Since the FLAG peptide (DYKDDDDK) is a commonly used epitope tag, Stratagene developed the TagDetect FLAG Protein Dipstick. It can be used to monitor expression of FLAG-tagged proteins in either prokaryotic or eukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps, and to identify proteins following immun
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
2. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
3. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
9. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
10. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
11. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive and Rapid Detection FLAG Tagged Fusion Proteins

(Date:12/25/2014)... The report “Service Quality Management and Telco ... Type - Global Advancements, Worldwide Forecasts & Analysis (2014-2019)” ... and forecasting of revenues. , Browse 152 market ... in-depth TOC on “Service Quality Management (SQM) and Telco ...
(Date:12/24/2014)... , Dec. 24, 2014   BioLife Solutions , Inc. ... marketer of proprietary clinical grade hypothermic storage and ... products for cells and tissues  ("BioLife" or the "Company"), ... of Stockholders on May 4, 2015 (the "Annual Meeting"). ...
(Date:12/24/2014)... MA (PRWEB) December 23, 2014 ... is designed to introduce cutting edge communication technology, ... of platform upgrades. This webinar series demonstrates online ... quest to leverage web conferencing’s most innovative and ...
(Date:12/24/2014)... Nashville Fertility Center announced that Dr. George A. Hill ... Medicine (ASRM), a leading professional organization committed to advancing knowledge ... as treasurer, Dr. Hill also fills a seat on the ... mission of ASRM. , Dr. Hill has actively supported ...
Breaking Biology Technology:Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4BioLife Solutions Sets Date for Annual Meeting of Stockholders 2SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3
... July 8 Knowledge Enterprises, Inc.,and The ... of The,2007-2008 Orthopaedic Industry Annual Report, the ... data. The nearly 200,page Annual Report summarizes ... Joint Replacement, Fracture Repair, Arthroscopy/Soft,Tissue Repair, Spinal ...
... 8 Telik, Inc.,(Nasdaq: TELK ), today ... Chemistry Letters describing a novel class of small ... treatment for diabetes.,These unique compounds are the result ... optimize the original lead structure identified through,the application ...
... US-made bulk API, BATON ROUGE, La., ... (NYSE: ALB ), a leading global ... active pharmaceutical,ingredients (APIs), has signed a global ... As per the agreement, effective,immediately, Albemarle has ...
Cached Biology Technology:Knowledge Enterprises, Inc. and The Institute for Orthopaedics(TM) Release The 2007-2008 Orthopaedic Industry Annual Report 2Telik Announces Publication of Diabetes Research in Bioorganic & Medicinal Chemistry Letters 2Albemarle Enters Ibuprofen Agreement with Dr. Reddy's 2Albemarle Enters Ibuprofen Agreement with Dr. Reddy's 3
(Date:12/22/2014)... , Dec. 22, 2014  The 2014 Holiday Season ... as Acuity Market Intelligence reports that the long anticipated ... forecasts that intensifying demand for smart phones, tablets, and ... global market of 2.5 billion users with nearly 4.8 ...
(Date:12/19/2014)... Calif. , Dec. 18, 2014  23andMe, Inc., the leading ... that pinpoints fine-scale differences in genetic ancestry of individuals from ... Since immigrants first arrived more than four hundred years ago, ... a meeting place for peoples from different continents. This study ...
(Date:12/17/2014)... FORT BELVOIR, Va. , Dec. 15, 2014 ... make it easier to detect and prevent counterfeit microcircuits ... the agency started performing an in-house microcircuit anti-counterfeit initiative ... of purchased microcircuits while increasing their reliability throughout the ...
Breaking Biology News(10 mins):First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3
... -- MacaInfo.org, a website that is devoted to answering the ... has just launched its brand new and easy-to-navigate site. The ... about maca for athlets , men and women. ... Maca grown in popularity? Although maca has recently ...
... 2013 The University of Chicago (UChicago) and Ben-Gurion University ... of ambitious research collaborations that apply the latest discoveries ... for making clean, fresh drinking water more plentiful and ... Sunday following a meeting in Jerusalem among Israeli President ...
... MARC (Maximizing Access to Research Careers) Program has announced ... Conferences held in July, 2013. These awards are meant ... and scientists into the mainstream of the basic science ... at the FASEB Science Research Conferences. This year MARC ...
Cached Biology News:MacaInfo.org Offers In-Depth Answers the 20 Most Common Questions People Have about Maca 2Ben-Gurion U. and UChicago research collaboration targets water resource innovations 2Ben-Gurion U. and UChicago research collaboration targets water resource innovations 3Ben-Gurion U. and UChicago research collaboration targets water resource innovations 4
This Universal 24" X 18" (61 X 45.7cm.) shaker platforms will accept any Thermo Forma shaker flask clip....
...
prototype Nco I...
Based on Silica membrane matrix beads. The kit is designed for the purification of DNA (20 bp - 100 bp) from TAE/TBE agarose gels. Run time is approximately 45 minutes....
Biology Products: