Navigation Links
Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins


Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Detection of expressed tags made easy

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang
Stratagene

Stratagenes TagDetect FLAG Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusion proteins. To visualize results, the kit uses a colloidal-gold (CG) labeled anti-FLAG M2 monoclonal antibody in a dipstick format. This highly specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, and it will not cross react with non-specific prokaryotic and eukaryotic proteins. It is sensitive and fast: Detect as little as 10 ng/ml of expressed FLAG-tagged fusion protein in just 10 minutes.

Epitope-tagging technology provides a powerful means to functionally analyze genes in eukaryotic cells. With this technique, a tag-specific antibody is used to target and analyze the fusion protein within the cell, without making a specific antibody to each new protein under study.1

Epitope tags are usually short polypeptides with defined sequences, which can be terminally or internally incorporated into the protein of interest. Sequences coding for common epitopes have been incorporated into a variety of cloning and expression vectors. Since the FLAG peptide (DYKDDDDK) is a commonly used epitope tag, Stratagene developed the TagDetect FLAG Protein Dipstick. It can be used to monitor expression of FLAG-tagged proteins in either prokaryotic or eukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps, and to identify proteins following immun
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
2. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
3. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
9. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
10. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
11. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive and Rapid Detection FLAG Tagged Fusion Proteins

(Date:11/21/2014)... England , Nov. 21, 2014   TRU-D SmartUVC ... will feature their superbug-killing UVC automated disinfection robot, TRU-D SmartUVC, ... this week from Nov. 23 to 26 at Stand 23. ... is the United Kingdom,s largest infection-related ... infection prevention societies to one of the leading events of ...
(Date:11/21/2014)... Angeles, CA (PRWEB) November 20, 2014 ... “A Short Walk to the Other Side” published ... an “Award-Winning Finalist in the “Fiction: Short Story” category ... , Jeffrey Keen, President and CEO of USA Book ... from mainstream and independent publishers, including Simon & Schuster, ...
(Date:11/21/2014)... 2014 The November 2014 issue of ... open access journal featuring the following articles:, ... preserve photoresponsiveness in the retina , DNA ... hybridization using chromogenic substrates in zebrafish , ... protein electrophoresis , Nuclear LC3-positive puncta in ...
(Date:11/21/2014)... 2014 Deep Research Report on ... professional and in-depth research report on the Sodium ... like its definition, classification, application, and industry chain ... covers the international market analysis, including China’s domestic ... US, Europe, Asia, China, Japan etc. regions) industry ...
Breaking Biology Technology:TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 2TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 2Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 3Mystic Ink 's Short Story Collection “A Short Walk to the Other Side” Honoring Ray Bradbury Praised As Award-Winning Finalist In USA Best Book Awards 4BioTechniques: The International Journal of Life Science Methods Announces the Publication of the November 2014 Issue—Available in Print and Online 2BioTechniques: The International Journal of Life Science Methods Announces the Publication of the November 2014 Issue—Available in Print and Online 3Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 2Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 3
... In a merging of complementary companies, Cytochroma, Inc ., ... has acquired Proventiv Therapeutics, LLC, of Madison, and the combined ... capital from Canadian and American investors. , ,Proventiv was founded ... International , which was acquired last year by Genzyme ...
... Foundry , a provider of media communications technology, will be ... and family get in touch with their loved ones serving ... Wisconsin Department of Veterans Affairs to offer Mediasite ... , ,The messages can be recorded with both audio and ...
... recent legislative setbacks, proponents of net neutrality said their ... of the United States Senate not to take up ... are included. , ,The pro-neutrality group It'sOurNet , ... to reject the telecommunications bill following a tie vote ...
Cached Biology Technology:Canadian firm buys Proventiv Therapeutics, makes ex-Bone Care exec CEO 2Canadian firm buys Proventiv Therapeutics, makes ex-Bone Care exec CEO 3Net neutrality will be back, supporters vow 2Net neutrality will be back, supporters vow 3Net neutrality will be back, supporters vow 4
(Date:11/10/2014)... U.S. store shelves in early 2010, and people have ... packets can be tossed into a washing machine without ... The convenience, though, has come with risks for young ... Children,s Hospital found that from 2012 through 2013, U.S. ... than 6 years of age swallowing, inhaling, or otherwise ...
(Date:11/7/2014)... , November 6, 2014 Leading ... for better mobile security revolutionizing online transactions.  Companies in focus ... Holdings Ltd. (NYSE: BABA ), Google Inc. (NASDAQ: ... ), eBay Inc. (NASDAQ: EBAY ) and MasterCard ... NXTD and NXTDW), a biometric authentication company focused on ...
(Date:11/6/2014)... when that predator runs so fast that it essentially blinds ... the fastest creature on Earth. Some of these half-inch-long beetles ... miles per hour). The fastest human can do about five ... beetle, a person would have to hit 480 miles per ... peak speeds, everything becomes a blur. They can,t gather enough ...
Breaking Biology News(10 mins):Study finds laundry detergent pods, serious poisoning risk for children 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6The tiger beetle: Too fast to see 2
... of Iowa researchers have found the existence of a new, ... find the genetic cause, which investigators hope will increase understanding ... the Nov. 9 issue of the Archives of Ophthalmology ... area of high-resolution central vision that is needed to read ...
... , , , ... www.kornberg.com ) has converted part of an existing building (Keen ... innovative stem cell core facility for the University of California, ... , "A key project element was to ensure ...
... WASHINGTON, Dec. 10, 2009 Arup K. SenGupta, Ph.D., ... Lehigh University in Bethlehem, Pa., has been chosen to ... administered by the American Chemical Society, is given to ... improve public health through their work in the chemical ...
Cached Biology News:New inherited eye disease discovered 2Kornberg Associates | Architects Completes UC Riverside Stem Cell Core Facility Project 2
RABBIT ANTI HUMAN APP (KP1 DOMAIN) Immunogen: Synthetic peptide composed of amino acids 292-312 from the KPI domain of human APP....
Anti-Goat HRP-AEC Cell & Tissue Staining Kit...
... CultureWell cell culture systems provide ... cultured cells for staining and ... silicone gaskets preassembled with standard ... plates. The entire system is ...
... Zero Background/Kan Cloning Kit includes ... resistance for selection in E. ... positive selection to eliminate high ... of the reagents you need ...
Biology Products: