Navigation Links
Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins


Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Detection of expressed tags made easy

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang
Stratagene

Stratagenes TagDetect FLAG Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusion proteins. To visualize results, the kit uses a colloidal-gold (CG) labeled anti-FLAG M2 monoclonal antibody in a dipstick format. This highly specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, and it will not cross react with non-specific prokaryotic and eukaryotic proteins. It is sensitive and fast: Detect as little as 10 ng/ml of expressed FLAG-tagged fusion protein in just 10 minutes.

Epitope-tagging technology provides a powerful means to functionally analyze genes in eukaryotic cells. With this technique, a tag-specific antibody is used to target and analyze the fusion protein within the cell, without making a specific antibody to each new protein under study.1

Epitope tags are usually short polypeptides with defined sequences, which can be terminally or internally incorporated into the protein of interest. Sequences coding for common epitopes have been incorporated into a variety of cloning and expression vectors. Since the FLAG peptide (DYKDDDDK) is a commonly used epitope tag, Stratagene developed the TagDetect FLAG Protein Dipstick. It can be used to monitor expression of FLAG-tagged proteins in either prokaryotic or eukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps, and to identify proteins following immun
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
2. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
3. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
9. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
10. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
11. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive and Rapid Detection FLAG Tagged Fusion Proteins

(Date:1/15/2014)... Freeslate, Inc ., the leading provider ... Limited, one of India’s top five pharmaceutical companies, has ... for high throughput solid form screening. , ... wide range of quality, affordable generic and branded formulations ...
(Date:1/14/2014)... 2014 Histogen, Inc., a regenerative medicine ... grown under simulated embryonic conditions, today announced that they ... Medical, Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary ... agreement is an amendment to the existing license between ...
(Date:1/14/2014)... Md. , Jan. 14, 2014  Organovo announced today ... the National Institutes of Health (NIH) to help scientists ... treatments to patients on a faster timeline. Organovo is ... (NCATS) and the National Eye Institute (NEI) to develop ...
(Date:1/14/2014)... Ontario , Jan. 14, 2014   Kinaxis ®, provider ... SCM ) and sales and operations planning ( S&OP ) ... Biomanufacturing Summit , which will be held at the ... the conference, join Kinaxis customer Elisabeth Kaszas , Director ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... Research Institute (IDRI) announces today the appointment of H. ... new member of the IDRI board of directors.  Parker ... having served as President and CEO of Targeted Genetics ... Corp. "I look forward to working with ...
... FORT WORTH, Texas, Feb. 15, 2011 The Center ... Center recently received a three-year $3.5 million grant ... leader in forensic DNA testing systems, to train researchers ...  This first of its kind accelerated academic certification program ...
... 15, 2011 Neuralstem, Inc. (NYSE Amex: ... I. Westreich to its Board of Directors. With his ... majority of independent directors. (Logo: http://photos.prnewswire.com/prnh/20061221/DCTH007LOGO) ... of Directors and the Company,s Chief Science Officer, welcomed ...
Cached Biology Technology:IDRI Announces Appointment of H. Stewart Parker as Chief Executive Officer 2$3.5 Million Life Technologies Grant Will Help Train, Certify Forensic Scientists at UNT Health Science Center 2$3.5 Million Life Technologies Grant Will Help Train, Certify Forensic Scientists at UNT Health Science Center 3$3.5 Million Life Technologies Grant Will Help Train, Certify Forensic Scientists at UNT Health Science Center 4Former Capital One Director and Business Leader Stanley I. Westreich Joins Neuralstem's Board 2Former Capital One Director and Business Leader Stanley I. Westreich Joins Neuralstem's Board 3
(Date:4/17/2014)... to treat tuberculosis could be the basis for a ... fungal infections and parasites, yet evade resistance, according to ... , Led by U. of I. chemistry professor Eric ... SQ109 attacks the tuberculosis bacterium, how the drug can ... malaria and how targeting multiple pathways reduces the ...
(Date:4/17/2014)... a word for chili peppers. Information about archaeological remains ... study of the appearance of words for chili peppers ... were domesticated and highlight the value of multi-proxy data ... al.) of nine papers presented in a special feature ... of Sciences on plant and animal domestication edited ...
(Date:4/17/2014)... NJ. April 16, 2014. Kessler Foundation has been ... million from the Department of Defense Spinal Cord ... principal investigator for the randomized, double-blinded, controlled, multi-site ... bone and muscle strength after spinal cord injury. ... & Engineering Research at Kessler Foundation. Two additional ...
Breaking Biology News(10 mins):Multitarget TB drug could treat other diseases, evade resistance 2Chickens to chili peppers 2Chickens to chili peppers 3Kessler Foundation awarded Department of Defense grant for spinal cord injury research 2
... Calif.) Calling the results "extremely surprising," researchers from ... University report that methane gas concentrations in the Gulf ... months after a massive release occurred following the Deepwater ... study, led by oceanographers John Kessler of Texas A&M ...
... team took a significant step forward in understanding ... pollutants and some other gases, except carbon dioxide. ... with some studies suggesting the self-cleaning power of ... changes, while others suggest greater stability. And what ...
... scientists "build" new biological systems, Princeton University scientists have ... the growth of living cells. The team of ... and the scientists showed that they can produce substances ... proteins produced by nature,s own toolkit. "What ...
Cached Biology News:UCSB, Texas A&M scientists find methane gas concentrations have returned to near-normal levels 2NOAA-led team measures atmosphere's self-cleaning capacity 2NOAA-led team measures atmosphere's self-cleaning capacity 3Princeton scientists construct synthetic proteins that sustain life 2Princeton scientists construct synthetic proteins that sustain life 3
... Cell Receptor-P1, clone 1C10 ... Hybridoma produced by the fusion ... immunized (BALB/c X C57BL/6)F1 (CbyB6F1) ... mouse myeloma cellline. ...
... Mouse monoclonal [YPF19] to Yersinia pestis ... detect Yersinia pestis . ... Yersinia pestis vaccine strain ... antibody recognises Y.pestis F1 capsular antigen. ...
Rabbit polyclonal to ZFP200 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGKSFNHKTNLNKHER (Human) Entrez Gene ID: 7752 Swiss Protein ID: P98182...
Anti-human C3a/C3a des-Arg (neo-epitope), Clone 2991, Monoclonal Antibody...
Biology Products: