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Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins


Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Detection of expressed tags made easy

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang
Stratagene

Stratagenes TagDetect FLAG Protein Dipstick is an easy-to-use assay kit for detecting FLAG epitope-tagged fusion proteins. To visualize results, the kit uses a colloidal-gold (CG) labeled anti-FLAG M2 monoclonal antibody in a dipstick format. This highly specific monoclonal antibody detects the FLAG epitope regardless of its location in fusion proteins, and it will not cross react with non-specific prokaryotic and eukaryotic proteins. It is sensitive and fast: Detect as little as 10 ng/ml of expressed FLAG-tagged fusion protein in just 10 minutes.

Epitope-tagging technology provides a powerful means to functionally analyze genes in eukaryotic cells. With this technique, a tag-specific antibody is used to target and analyze the fusion protein within the cell, without making a specific antibody to each new protein under study.1

Epitope tags are usually short polypeptides with defined sequences, which can be terminally or internally incorporated into the protein of interest. Sequences coding for common epitopes have been incorporated into a variety of cloning and expression vectors. Since the FLAG peptide (DYKDDDDK) is a commonly used epitope tag, Stratagene developed the TagDetect FLAG Protein Dipstick. It can be used to monitor expression of FLAG-tagged proteins in either prokaryotic or eukaryotic cells, to follow FLAG-tagged proteins during purifica- tion steps, and to identify proteins following immun
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