Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

which is part of CGAP.

Conclusion

SAGE is a well-recognized method of gene-expression profiling. The information gathered from SAGE analysis enables a clear view of the genes associated with normal, developmental, and disease states. Researchers require an efficient means of accessing the sequence information contained in the concatamer of transcript tags. ABI PRISM 3100 and 3700 DNA sequencers, coupled with BigDye terminator chemistry, provide a versatile approach to generating ditag sequences, and make the SAGE application readily accessible to disease investigators.

References

1.Velculescu, V.E., Zhang, L., Vogelstein, B., and Kinzler, K.W. 1995. Serial analysis of gene expression. Science . 270:484487.

2.Kozian, D.H., and Kirschbaum, B.J. 1999. Comparative gene-expression analysis. Trends in Biotechnology . 17:7378.

3.Johns Hopkins University SAGE Web site: http://www.sagenet.org. Schematic of SAGE method. April 2001.

4.Dr. K. Kinzler, Dr. B. Vogelstein, Johns Hopkins University, Baltimore, MD.

5.National Institutes of Health Genetic Sequence Database: http://www.ncbi.nlm.nih.gov/ Genbank/ and http://www.ncbi.nlm. nih.gov/UniGene/index.html. April 2001. 6.Lash, A.E., Riggins, G.J. et al. 2000. SAGEmap: A public gene expression resource. Genome Research. 10:10501060.

*Invitrogen Corporation has released I-SAGE, the first pre-assembled kit for performing SAGE technology. This all-inclusive kit provides sufficient pre-tested materials to generate up to six SAGE libraries for subsequent sequencing.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:12/19/2014)... In contrast to traditional LED lights where ... Valoya uses proprietary LED technology to incorporate their spectra ... which is critical in low proximity installations. The Valoya ... its lifetime which is up to four times longer ... investment cost was clearly higher when compared to traditional ...
(Date:12/19/2014)... -- Decision Resources Group finds that the Asia ... through 2023 as the aging population, increasing adoption of ... all spur procedure volumes. Growth will be particularly rapid ... and India , where rising ... a larger proportion of the population. Other ...
(Date:12/19/2014)... 2014 Relmada Therapeutics, Inc. (OTCQB: RLMD), a ... chronic pain, announced the results of its annual general ... a transformational year in Relmada,s history and it set ... said Sergio Traversa , chief executive officer of ... key financial, human resource and clinical development objectives that ...
(Date:12/17/2014)... 2014 “Never say, ‘I cannot ... Marburg’s Department of Physics, advises young women pursuing ... released SPIE Women in Optics 18-month planner ... to ask questions.” , In the annual planner, ... occupations ranging from university professor and laboratory researcher ...
Breaking Biology Technology:Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3Relmada Therapeutics Announces Results of Annual Shareholders Meeting 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3
... 30 /PRNewswire-FirstCall/ - ICBS Limited,(PINKSHEETS: ICBM), today announced ... Bacteria Bank Ltd., is a Bio medical ... for use in medical research, located,in Montreal Canada. ... a world,renowned bacteriologist. Dr. Karl Weiss is ...
... 30 CryoCor, Inc. (Nasdaq:,CRYO), a medical device ... today that Edward F. Brennan, President and Chief,Executive ... are,scheduled to present at the CIBC World Markets ... Tuesday, November 6, 2007 at 3:40 p.m. ET ...
... ST. LOUIS, Oct. 30 Sigma, a division ... provide researchers,additional Cell Biology solutions through the new ... http://www.sigma.com/ionchannelsolutions ). In addition to the most,comprehensive product ... provide a,wealth of scientific information to assist in ...
Cached Biology Technology:ICBS acquires 30% interest in Bacteria Bank Ltd. 2CryoCor to Present at CIBC World Markets 18th Annual Healthcare Conference 2Sigma Launches The New Ion Channel Solutions Brochure and Web Site 2
(Date:12/4/2014)... BEACH GARDENS, Fla. , Dec. 3, 2014 ... management solutions, today announced that its DigitalPersona ® ... 4500 fingerprint readers have been deployed throughout First ... , South Carolina ... headquartered in Southern Pines, North Carolina ...
(Date:11/21/2014)... -- According to a new market research ... Access Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), ... Geography - Global Forecasts to 2020", published by MarketsandMarkets, ... around $25 Billion in 2014 and is expected to ... of 8.69%. Browse 116 market data Tables ...
(Date:11/21/2014)... -- C-Labs LLC, a leading provider of remote and ... announced the appointment of John Traynor to ... advisor to the firm, Mr. Traynor will now oversee ... out of the C-Labs office in Redmond, ... , Chief Executive Officer. Photo - ...
Breaking Biology News(10 mins):First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2
... 2011 In a new study scientists at NYU Langone ... be improved. The new findings, published online November 20, 2011, ... the loss of smell due to aging or disease. ... A. Wilson, PhD, professor of child and adolescent psychiatry at ...
... University of British Columbia researchers reveals how the effect of ... fisheries practices. "Fisheries are already providing fewer fish and ... says Rashid Sumaila, principal investigator of the Fisheries Economics Research ... "We could be earning interest, but instead we,re fishing away ...
... Suitable habitat for native fishes in many Great Plains ... from the High Plains aquifer and scientists analyzing the ... Results of their study have been published in the ... be replenished seasonally with rain and snow, these regional aquifers ...
Cached Biology News:A failing sense of smell can be reversed 2Effects of climate change to further degrade fisheries resources: UBC researchers 2Great Plains river basins threatened by pumping of aquifers 2Great Plains river basins threatened by pumping of aquifers 3
...
BD Primaria 35 mm Cell Culture Dish with Easy-Grip design, surface-modified polystyrene for enhanced cell culture. (20/sp, 200/ca) Packaging: 20 / sleeve, 500 / case ...
...
BD Falcon insert HTS 96 well, square A-bottom with lid. PET membrane, sterile. (5/ca) Packaging: 5 / pack, 5 / case ...
Biology Products: