Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

ity by offering medium-to-high throughput.

Capillary lengths of 36 cm, 50 cm, and 80 cm on the 3100 Genetic Analyzer afford flexible run times and read lengths. This system is designed with 16 capillaries, and run times can be determined, depending on insert size. Typically, a rapid run will read tag sequences to 550 bp with 98.5% accuracy. We recommend the 80-cm array for long-read runs for inserts of 1 kb or larger. (See Table 1 for throughput.)

Using standard run conditions, the 50-cm array on the ABI PRISM 3700 DNA analyzer will provide long reads with POP-5 polymer over a 3-hour run time. Three 24-hour running periods, therefore, will yield almost all the sequence information required to profile one tissue type (See Table 1).

Analysis

When sequencing and basecalling are complete, the investigator needs to assess the presence and frequency of ditags. Various software tools are available to analyze the ditag sequence and determine transcript abundance. When all sequence files have been processed, it is possible to view tag abundances, match tags to reference sequences, and compare tag abundances from multiple projects. Analysis generally involves matching the tag information against the GenBank-UniGene5 database and generating a reference list. Project tags are compared with this list to identify matches of known genes and other sequences.

The National Institutes of Health (NIH) has established SAGEmap, a public repository and resource for SAGE data.6 Originally developed by researchers at the National Center for Biotechnology Information (NCBI) in Bethesda, MD, SAGEmap was designed to archive SAGE data produced through the Cancer Genome Anatomy Project (CGAP). However, it also accepts submissions of SAGE sequence from any source. The National Cancer Institute (NCI) has chosen to use SAGE exclusively for The Human Tumor Gene Index (hTGI) initiative,
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:10/22/2014)... KONG , Oct. 22, 2014  aTyr Pharma ... that rare disease expert John C. McKew , ... McKew brings more than two decades of expertise in ... Institutes of Health, Wyeth Research and Genetics Institute, Inc. ... aTyr,s efforts to expand and translate its novel Physiocrine ...
(Date:10/20/2014)... Inc. (TSXV: BRM) ("Biorem" or "the Company") today announced four new ... and provides a good start to Q4.  The orders are for ... and one in the Middle East . ... Peter Bruijns , President & CEO. "Total bidding activity and ... have been for any complete year since the company has tracked ...
(Date:10/20/2014)... Earle Martin , Chief ... that Ellen Teplitzky, an experienced attorney specializing in ... industry, has joined the firm as Director of ... practice. NDA Partners provides legal services, including ... to top law firms and their clients in ...
(Date:10/19/2014)... The Asia-Pacific Bromine Market report defines and segments ... forecast of revenue. , Browse through the TOC ... get an idea of the in-depth analysis provided. ... in the Asia-Pacific bromine market, and is supported ... http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , Bromine is a volatile and ...
Breaking Biology Technology:aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3
... Reportlinker.com announces that a new market research ... Enzymes: Sustainable Alternatives with Remarkable ... Enzymes are among the most ... host of different roles in a wide ...
... Oppenheimer & Co. Inc., a unit of Oppenheimer Holdings, ... the firm as Managing Director in the Healthcare group of ... its life science business. He will report to Brian McCarthy, ... Since 2003, Matthias had worked at JMP Securities, where ...
... 2011 Children at Paterson Public School No. 5 ... of more than 200 volunteers from Roche, New Jersey ... organizers from non-profit KaBOOM!. The new playground provides the ... as well as children living in the neighborhood with ...
Cached Biology Technology:Reportlinker Adds Enzymes: Sustainable Alternatives with Remarkable Scope 2Reportlinker Adds Enzymes: Sustainable Alternatives with Remarkable Scope 3Designed by Kids, Built by Volunteers: Roche, New Jersey Community Development Corporation, and KaBOOM! Build New Playground in Just One Day for Paterson Public School Children 2Designed by Kids, Built by Volunteers: Roche, New Jersey Community Development Corporation, and KaBOOM! Build New Playground in Just One Day for Paterson Public School Children 3Designed by Kids, Built by Volunteers: Roche, New Jersey Community Development Corporation, and KaBOOM! Build New Playground in Just One Day for Paterson Public School Children 4
(Date:10/15/2014)... Fred Alt will be awarded the 44th Rosenstiel Award ... for his pioneering research exploring the mechanisms of genomic ... cancer cells. Alt is the second alumnus to win ... Nobel Prize in 2003. , Alt is the Charles ... at Harvard Medical School and an investigator at the ...
(Date:10/14/2014)... 14, 2014)—It,s been millions of years since T. ... led by Ohio University scientists is breathing life back ... through dinosaur snouts. The research has important implications for ... but to enhance the sense of smell and cool ... said Ohio University doctoral student Jason Bourke, lead author ...
(Date:10/14/2014)... oil supplements, rich in omega-3 fatty acids, do not reduce ... the heart can beat as fast as 150 beats a ... Montreal Heart Institute were published in the Journal of ... For the trial, 337 patients with atrial fibrillation not receiving ... fish oil a day or to placebo for up to ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3
... Symposium on Multimodality Cardiovascular Molecular Imaging ... cell therapy, leading-edge research in imaging technology and ... of cardiovascular receptors, vascular biology, myocardial metabolism and ... Partnership for Critical Markers of Disease will host ...
... million year old fossils reveal much earlier origins of modern ... The chances of an octopus corpse surviving long enough to ... only a single fossil species was known, and from fewer ... octopus is. Even if you have never encountered one in ...
... - NASA scientists analyzing the dust of meteorites have discovered ... on its most basic, molecular level. "We found more ... created in space and brought to Earth by meteorite impacts ... of NASA,s Goddard Space Flight Center in Greenbelt, Md. "By ...
Cached Biology News:SNM Symposium on Multimodality Cardiovascular Molecular Imaging 2Cretaceous octopus with ink and suckers -- the world's least likely fossils? 2NASA researchers find clues to a secret of life 2NASA researchers find clues to a secret of life 3
...
Request Info...
Human RUNX2/CBFA1 MAb (Clone 232902)...
... Peroxidase Conjugate, 2 ml. ... enzyme molecules for use ... situ hybridization applications.High specificity ... Labeling & Detection, Protein ...
Biology Products: