Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

ity by offering medium-to-high throughput.

Capillary lengths of 36 cm, 50 cm, and 80 cm on the 3100 Genetic Analyzer afford flexible run times and read lengths. This system is designed with 16 capillaries, and run times can be determined, depending on insert size. Typically, a rapid run will read tag sequences to 550 bp with 98.5% accuracy. We recommend the 80-cm array for long-read runs for inserts of 1 kb or larger. (See Table 1 for throughput.)

Using standard run conditions, the 50-cm array on the ABI PRISM 3700 DNA analyzer will provide long reads with POP-5 polymer over a 3-hour run time. Three 24-hour running periods, therefore, will yield almost all the sequence information required to profile one tissue type (See Table 1).

Analysis

When sequencing and basecalling are complete, the investigator needs to assess the presence and frequency of ditags. Various software tools are available to analyze the ditag sequence and determine transcript abundance. When all sequence files have been processed, it is possible to view tag abundances, match tags to reference sequences, and compare tag abundances from multiple projects. Analysis generally involves matching the tag information against the GenBank-UniGene5 database and generating a reference list. Project tags are compared with this list to identify matches of known genes and other sequences.

The National Institutes of Health (NIH) has established SAGEmap, a public repository and resource for SAGE data.6 Originally developed by researchers at the National Center for Biotechnology Information (NCBI) in Bethesda, MD, SAGEmap was designed to archive SAGE data produced through the Cancer Genome Anatomy Project (CGAP). However, it also accepts submissions of SAGE sequence from any source. The National Cancer Institute (NCI) has chosen to use SAGE exclusively for The Human Tumor Gene Index (hTGI) initiative,
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:2/26/2015)... SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: SQD; ... commercializes proprietary technologies and products for advanced microarray diagnostics, ... fiscal first quarter ended December  31, 2014. All figures ... unless otherwise stated. "During the quarter ... existing customers and add to our sales pipeline," said ...
(Date:2/26/2015)... MENLO PARK, Calif. , Feb. 26, 2015 /PRNewswire/ ... has released the latest version of the Governance ... organizations accomplish governance, risk and compliance (GRC) tasks across ... has also been updated to help customers better align ... Organizations of the Treadway Commission ) requirements. In response ...
(Date:2/26/2015)... On behalf of biotech client ... Wilentz, Goldman & Spitzer, P.A. (Wilentz) secured a ... Genewiz Inc. in excess of $10 million. The jury ... secrets, and that it improperly hired one or more ... denying motions for judgment notwithstanding the verdict, a new ...
(Date:2/26/2015)... , Feb. 26, 2015 BioEnterprise today announced ... have attracted more than $2 billion in growth funding ... and national investors, strategic sources, state – including the ... of the $2 billion has been raised in the ... on the heels of the BioEnterprise Midwest Healthcare ...
Breaking Biology Technology:SQI Diagnostics Reports First Quarter Financial Results 2SQI Diagnostics Reports First Quarter Financial Results 3SQI Diagnostics Reports First Quarter Financial Results 4SQI Diagnostics Reports First Quarter Financial Results 5SQI Diagnostics Reports First Quarter Financial Results 6Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 2Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 3Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3More Than $2 Billion Invested in Northeast Ohio Bioscience Start-Up Companies Since 2002 2More Than $2 Billion Invested in Northeast Ohio Bioscience Start-Up Companies Since 2002 3More Than $2 Billion Invested in Northeast Ohio Bioscience Start-Up Companies Since 2002 4
... SAN CARLOS, Calif. and BROOMFIELD, Colo., Dec. 1 ... ARCA biopharma, Inc., a privately-held,biopharmaceutical company developing genetically-targeted ... announced that a special,meeting of Nuvelo,s stockholders will ... PT at Nuvelo,s headquarters at 201 Industrial Road, ...
... RIDGE, N.J., Dec. 1 Regado Biosciences, Inc.,announced today ... present at the 20th Annual Piper Jaffray Health Care ... at 10:10am (Eastern Standard Time).,Dr. Mazzo will discuss the ... clinical developments. , ...
... for nomination of candidates worldwide - , ... Technology Foundation of Japan today announced the two fields eligible ... prestigious awards in science and technology. The 2010 Japan Prize ... and biological production and environment. , , ...
Cached Biology Technology:Nuvelo and ARCA biopharma Advance Toward Completion of Merger 2Nuvelo and ARCA biopharma Advance Toward Completion of Merger 3Nuvelo and ARCA biopharma Advance Toward Completion of Merger 4Nuvelo and ARCA biopharma Advance Toward Completion of Merger 52010 Japan Prize to Honor Scientists and Researchers in Industrial Production/Production Technology and Biological Production/Environment Fields 22010 Japan Prize to Honor Scientists and Researchers in Industrial Production/Production Technology and Biological Production/Environment Fields 3
(Date:2/5/2015)... , Jan. 26, 2015   Epic Sciences , a ... today announced that Murali Prahalad , Ph.D., president and ... World Conference (PMWC) 2015: Silicon Valley, which is taking ... View, Calif. on January 26-28, 2015. ... of Age as Biomarkers." Last year, Epic Sciences was a ...
(Date:2/5/2015)... 2015 Research and Markets ( ... "Global Biometrics Market (2014-2020): Market Forecast By ... report to their offering. , ... is anticipated to overtake North ... government spending towards IT security, government biometric based ...
(Date:1/22/2015)... TORONTO , Jan. 13, 2015 /PRNewswire/ - Today, FindBiometrics, the ... announced the launch of its new website design. ... in its infancy", says Peter O,Neill , founder and ... ever, the industry needs involvement from the key players on ...
Breaking Biology News(10 mins):Epic Sciences to Present at Personalized Medicine World Conference 2015 on the Use of Circulating Tumor Cells as Cancer Biomarkers 2Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 2Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 3Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2
... cells that may help to kill tumors with heat. Prostate ... a combination of heat and an anti-cancer drug that damages ... of heat to switch off essential survival mechanisms in human ... principles are still unclear. In a recent publication in ...
... by scientists at Queen,s University Belfast could lead to ... discovery could see the development of new therapies, which ... well as treating the tumour itself., Researchers at Queen,s ... that the non-cancerous tissue, or ,stroma, surrounding cancers of ...
... , PROVIDENCE, R.I. [Brown University] In a touch-screen display ... clear. Engineers employ transparent thin films of indium tin oxide ... only half its resume. They must also be as cheap ... researchers from Brown University and ATMI Inc. report the best-ever ...
Cached Biology News:Queen's University Belfast makes significant cancer breakthrough 2Chemists advance clear conductive thin films 2
... Sequencing Quality 96 Well ... BAC template preparation from 1.5ml ... suitable for automated fluorescent sequencing ... DNA; Utilizes ProPrep strategy which ...
...
TLN2 Antibody...
Olig2 Immunogen: Synthetic peptide from rat. Available Date: 9-19-2006...
Biology Products: