Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

h degree of accuracy that transcript analysis of short 9- or 10-bp sequences demands. This accuracy, coupled with the sensitive detection capacity of the ABI PRISM 3100 or 3700 DNA sequencers, ensures a fast and accurate read. BigDye Terminator v 3.0 enables even greater signal uniformity for samples that show a tendency toward signal loss in longer fragments.

Complete Automation

Both the 3100 and 3700 DNA sequencers are designed for more than 24 hours of unattended operation. Human intervention is required only to place the 96- or 384-well sample trays on the instrument, and to replenish the polymer supply. All other steps, including capillary filling, sample loading, capillary rinsing, data extraction, and analysis are completely automated.

SAGE Sequence Protocol

In experiments using typical sequencing methods, along with the ABI PRISM 3100 Genetic Analyzer and 3700 DNA Analyzer for sequencing, Applied Biosystems scientists produced reproducible and accurate ditag sequences.

Samples were prepared from mRNA derived from a cell line, Mcf-7 (human breast adenocarcinoma). A sequence content of approximately 26 ditags was obtained using both the standard protocol with POP-5 polymer on the 3700 DNA Analyzer, and the Rapid Read protocol on the 3100 Genetic Analyzer. For optimal sequence efficiency, the insert length should be sufficient to fully utilize the length-of-read for the instrument platform and BigDye chemistry.

Throughput

Throughput is an important consideration when choosing a platform for sequence analysis. The elimination of gel tracking and other problems that can arise from slab-gel sequencing greatly improves the sequencing capacity of the average research laboratory. Throughput requirements depend on the quantity of tags to be analyzed. ABI PRISM DNA sequencers provide this flexibil
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:11/26/2014)... -- Theravalues Corporation annuncia con orgoglio il lancio di ... Hi Europe 2014 (dal 2 al 4 dicembre, ... curcumina con la maggiore biodisponibilità di sempre, con una ... dalle norme europee. La curcumina è un ... Curcuma longa ) che è stata associata con attività ...
(Date:11/24/2014)... BUENA, N.J. , Nov. 24, 2014  IGI ... Jersey based specialty generic pharmaceutical company, today ... application (ANDA) to the U.S. Food and Drug Administration ... submissions in 2014 to seven, with eighteen submissions now ... Grenfell-Gardner , President and CEO of the Company, commented, ...
(Date:11/24/2014)... , Nov. 24, 2014  Last week, Representatives ... Butterfield (D-NC) took a bold bipartisan step ... introducing the OPEN Act. Original co-sponsors of the ... The Act will incentivize drug makers and innovators ... diseases and pediatric cancers, which opens the door ...
(Date:11/24/2014)... 2014   First ... Elsevier , a world-leading provider of scientific, ... announce the launch of the latest title in the ... in Food Science . ... a new platform to keep up-to-date with the expanding volume ...
Breaking Biology Technology:La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 2La curcumina con la maggiore biodisponibilità di sempre farà il suo debutto in Europa 3IGI Laboratories, Inc. Announces ANDA Submission 2OPEN Act Introduced to Repurpose Drugs for Rare Diseases 2Elsevier Announces Launch of New Journal: Current Opinion in Food Science 2Elsevier Announces Launch of New Journal: Current Opinion in Food Science 3
... Inclinix, Inc. has announced today that it has ... (Nasdaq: DATA ) to offer the most ... device companies globally. , ... DATATRAK,s eClinical(TM) platform to enhance its services as a ...
... Md., April 30 /PRNewswire/ -- ... company developing novel, proprietary antibodies for the treatment of ... will host a conference call and audio webcast on ... (3:00 pm Central European Time) to discuss its financial ...
... Inc. (NASDAQ: AEZS , TSX: AEZ), a ... will announce its first quarter 2009 financial and operating ... The Company will host a conference call and webcast ... 3:30 p.m., Eastern Time. , Participants may access the ...
Cached Biology Technology:DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 2DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 3DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 4Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 2Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 3Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 4
(Date:11/4/2014)... , November 4, 2014   ... vertical market growth   Fuel3D , a ... closed a funding round totaling $6.4 million (£4 million). This ... funding secured earlier this year and paves the way for ... 2015. The funding round was led by Chimera ...
(Date:11/4/2014)... Lithuania , Nov. 4, 2014   ... technologies, today announced that the latest version of ... in the Ongoing MINEX evaluation organized ... of fingerprint algorithms using the INCITS 378 fingerprint ... mandatory requirement in public tenders in ...
(Date:11/3/2014)... Cancer Center study published in this month,s ... describes the activity of a recently discovered communication molecule ... It has been known to limit inflammation and the ... system: IL-37 inhibits the ability of the immune system ... mechanism that underlies IL-37,s effect on the immune system ...
Breaking Biology News(10 mins):Fuel3D Secures $6.4 Million in Expansion Funding 2Fuel3D Secures $6.4 Million in Expansion Funding 3Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 3PNAS: From HIV to cancer, IL-37 regulates immune system 2
... the University of California, Santa Cruz and the University ... advanced robotic surgery systems for use by major medical ... of final tests, five of the systems will be ... Hopkins University, University of Nebraska, UC Berkeley, and UCLA, ...
... can blame our taste buds. Our tongues apparently recognize and ... School of Medicine in St. Louis. They have found that variations ... the taste of fat. The study is the first to ... some people may be more sensitive to the presence of fat ...
... topic in health news. Doctors prescribe it for medical reasons, ... And while it,s known that too little iron can result ... neurodegenerative diseases. Now, researchers at UCLA have ... of iron early in life can affect the brain,s physical ...
Cached Biology News:'Open-source' robotic surgery platform going to top medical research labs 2Receptor for tasting fat identified in humans 2Receptor for tasting fat identified in humans 3Receptor for tasting fat identified in humans 4Receptor for tasting fat identified in humans 5Diet counts: Iron intake in teen years can impact brain in later life 2Diet counts: Iron intake in teen years can impact brain in later life 3
Designed for flow cytometric applications intended to identify...
MOUSE ANTI RAT RT1Bu / MOUSE Ia:BIOTIN Immunogen: Rat thymocyte membrane glycoproteins...
MOUSE ANTI GUINEA PIG T-CELLS (PAN):FITC Immunogen: Guinea pig peritoneal T-cells...
MOUSE ANTI RABBIT T LYMPHOCTYES Immunogen: Rabbit Thymocytes...
Biology Products: