Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

tools are used to identity and determine abundance. This process involves the following three steps:

1.Biotinylated primers are used to reverse-transcribe mRNA. The resultant cDNA is then digested with a frequent-cutting enzyme (anchoring enzyme AE). Using streptavidin-coated beads, the 3' end of the cDNA is bound and then split into two pools, A and B. A primer (A' and B') containing a recognition site for a type IIS restriction enzyme is ligated to the nucleotides in each of these pools.

2.Following digestion with a relevant type IIS tagging enzyme, eluted DNA fragments are ligated and amplified using the A' and B' primers. Following PCR, the anchoring enzyme removes the priming sites A and B. Concatamers are then formed and cloned into a vector. A ditag containing the sequence information of two independent cDNA tags is punctuated by the anchoring site.*

3.Cloned inserts are verified by agarose gel and sequenced. SAGE analysis tools then analyze these sequences. Finally, a comparison of the sequence data determines variations in expression patterns.

Applied Biosystems Capillary Electrophoresis Systems and SAGE Technology

Most SAGE investigations require analysis of more than 50,000 tags. Obtaining sequencing information quickly and accurately is vital. Applied Biosystems capillary electrophoresis systems and BigDye terminator chemistries offer an easy, hands-free approach to sequencing. ABI PRISM 3700 and 3100 DNA sequencers accurately and reliably determine the transcript information necessary to build a profile. ABI PRISM BigDye terminators provide signal uniformity and sensitivity. Together they offer a cost-effective solution to largescale transcript analysis using SAGE technology.

Signal Detection

Accurate basecalling at long readlengths is important for ditag analysis. BigDye terminators provide the hig
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:7/24/2014)... to materialise. Yet, scientists are making progress in ... One such approach relies on quantum dotsa kind ... electric field. A new study demonstrates that changing ... (TQDs) with electrical impulses can help better control ... be used as quantum information units, which would ...
(Date:7/24/2014)... Research and Markets has announced ... Analytical Instruments Market 2014-2018" report to their offering. ... Genomics is the study of the gene and its ... and functions of proteomes or sets of proteins by ... involves the mapping of genes and DNA sequencing to ...
(Date:7/24/2014)... 24, 2014 Three companies from the ... Livestrong’s Big C Competition. Out of 700 competition entrants, ... to the semi-final round. In this round twenty teams ... program, complete with mentoring from thought-leaders and medical entrepreneurs ... of the angelMD commitment to the Livestrong vision around ...
(Date:7/24/2014)... July 24, 2014 1000 balances – ... input on customers’ weighing needs and expertly narrows down ... of balances, scales or even terminals. , First, ... smallest weight (or select readability instead) and define the ... additional selection criteria narrows down the list to models ...
Breaking Biology Technology:Global Genomics and Proteomics Analytical Instruments Market 2014-2018: Key Vendors are Agilent, Bio-Rad , F. Hoffmann, La Roche, Illumina, PerkinElmer and Thermo Fisher Scientific 23 angelMD Startups Make Livestrong Big C Semi-finals 2
... knowingly misrepresented the ... company to ... filed a,proposed class-action lawsuit on behalf of CellCyte Genetics Corporation,(OTC ... and chief executive officer Gary A.,Reys, background, causing company stocks ...
... SAN DIEGO, Jan. 18 The San Diego ... has received the,highest score in three of four ... on Part I of the Major Facilities Grant,Application., ... earned scores of,99 for basic research and discovery, ...
... objects to implications in a New York Times article ... clinical,trials., The story, based on a separate article ... and Lilly as high-profile examples of how the,industry purportedly ... story inaccurate when it comes to Prozac -- the ...
Cached Biology Technology:Hagens Berman Sobol Shapiro: Lawsuit Filed Against CellCyte Genetics Corporation 2Hagens Berman Sobol Shapiro: Lawsuit Filed Against CellCyte Genetics Corporation 3SDCRM Receives Highest Score in Three of Four Categories from CIRM on Part I of Major Facilities Grant Application 2SDCRM Receives Highest Score in Three of Four Categories from CIRM on Part I of Major Facilities Grant Application 3In response to a New England Journal of Medicine article published yesterday and subsequent media coverage, Eli Lilly and Company issued the following statement: 2In response to a New England Journal of Medicine article published yesterday and subsequent media coverage, Eli Lilly and Company issued the following statement: 3In response to a New England Journal of Medicine article published yesterday and subsequent media coverage, Eli Lilly and Company issued the following statement: 4
(Date:7/25/2014)... New research at the University of Adelaide has opened the ... resistance to powdery mildew. , In Australia, annual barley production ... year. Powdery mildew is one of the most important diseases ... team have discovered the composition of special growths on the ... the fungus into the leaf. , The research, by the ...
(Date:7/25/2014)... central nervous system disease that is caused by ... the embryonic phase. Many patients have varying degrees ... retardation. Such problems decrease the patients, functional independence ... Physical Medicine and Rehabilitation Education and Research Hospital, ... SB, using the Pediatric Evaluation of Disability Inventory ...
(Date:7/25/2014)... that the age girls reach puberty is influenced by ,imprinted ... parent contributes the gene. This is the first evidence ... birth and details of this study were published today in ... period, known as menarche, is a marker for the timing ... onset of menses varies between girls, is an inherited trait, ...
Breaking Biology News(10 mins):New hope for powdery mildew resistant barley 2Could age of first period influence development of diseases in older women? 2
... provide key insights for scientists and medical researchers, but ... pose some key challenges. Researchers at North Carolina ... the parameters used in such models which may ... fertility. "These oscillatory systems are found in everything ...
... facility in the US, researchers at the University of Gothenburg have ... is to film how proteins work at molecular level. ... cells could be the key to cures for everything from cancer ... University of Gothenburg, and his research group were among the first ...
... Carrying a gun in bear country doesn,t mean you,re more protected ... out of Brigham Young University. A study led by BYU ... gun is no more effective in keeping people from injury or ... really isn,t about the kind of gun you carry, it,s about ...
Cached Biology News:New method for estimating parameters may boost biological models 2New method for estimating parameters may boost biological models 3More effective method of imaging proteins 2BYU study: Using a gun in bear encounters doesn't make you safer 2
... Versatile, modular, computer controlled pre-amplifier suitable for ... isolated electrode input for recording physiological signal ... and a transducer input for use with ... transducers. Additional options include a stimulus artefact ...
... Polystyrene covers can be used ... Ridged covers for stacking of ... covers for use with automated ... ridged and non-ridged, are available ...
... available for use with 96- or 384-well PCR plates ... ease of location on 96-tube plates End tabs ... of the plate* Efficient sealing is achieved with ... with screw or clip-down hot lid thermal cyclers ...
... be used with all 96-well ... stacking of covered plates ... with automated systems ... are available for use with ...
Biology Products: