Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

tools are used to identity and determine abundance. This process involves the following three steps:

1.Biotinylated primers are used to reverse-transcribe mRNA. The resultant cDNA is then digested with a frequent-cutting enzyme (anchoring enzyme AE). Using streptavidin-coated beads, the 3' end of the cDNA is bound and then split into two pools, A and B. A primer (A' and B') containing a recognition site for a type IIS restriction enzyme is ligated to the nucleotides in each of these pools.

2.Following digestion with a relevant type IIS tagging enzyme, eluted DNA fragments are ligated and amplified using the A' and B' primers. Following PCR, the anchoring enzyme removes the priming sites A and B. Concatamers are then formed and cloned into a vector. A ditag containing the sequence information of two independent cDNA tags is punctuated by the anchoring site.*

3.Cloned inserts are verified by agarose gel and sequenced. SAGE analysis tools then analyze these sequences. Finally, a comparison of the sequence data determines variations in expression patterns.

Applied Biosystems Capillary Electrophoresis Systems and SAGE Technology

Most SAGE investigations require analysis of more than 50,000 tags. Obtaining sequencing information quickly and accurately is vital. Applied Biosystems capillary electrophoresis systems and BigDye terminator chemistries offer an easy, hands-free approach to sequencing. ABI PRISM 3700 and 3100 DNA sequencers accurately and reliably determine the transcript information necessary to build a profile. ABI PRISM BigDye terminators provide signal uniformity and sensitivity. Together they offer a cost-effective solution to largescale transcript analysis using SAGE technology.

Signal Detection

Accurate basecalling at long readlengths is important for ditag analysis. BigDye terminators provide the hig
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:4/1/2015)... Holomic is introducing the HRDR-400, the ... and fluorescent rapid tests with one device. , ... readers and is already selling its HRDR-200 chromatographic and ... of its kind offering for manufacturers and end-users that ... Neven Karlovac, CEO of Holomic LLC, commented, "We are ...
(Date:4/1/2015)... Earlier this year, A.J. O’Connor Associates ... association that represents research-based life science organizations in New ... AJO’s Vice President of Business Development & Executive Coach, ... that attracted over 750 attendees. , In one ... old cancer survivor shared his moving story of how, ...
(Date:3/31/2015)... Fla. , March 31, 2015  Biotest Pharmaceuticals ... is pleased to announce the addition of its newest ... Suite 205 Conway, Arkansas . The ... doors for business on March 26 th 2015 ... centers to eighteen. "This facility represents ...
(Date:3/31/2015)... PA (PRWEB) March 31, 2015 Pittcon ... and Applied Spectroscopy, ended on March 12, in New ... at $21 million as estimated by the New Orleans ... more than 14,200 conferees and exhibitor personnel. Those ... of the conferee number. For the third year, the ...
Breaking Biology Technology:Holomic to Introduce the First Dual, Chromatographic and Fluorescent Rapid Test Reader, the HRDR-400 2HR Consulting Firm, A.J. O'Connor Associates Joins BioNJ – The Vibrant 400 Member Strong Life Sciences Industry Association - and Attends its Annual Dinner 2Biotest Pharmaceuticals Corporation Announces the Opening of its Newest Plasma Collection Facility in Conway, Arkansas 2Pittcon 2015 Hits the Big Easy in a Big Way 2Pittcon 2015 Hits the Big Easy in a Big Way 3Pittcon 2015 Hits the Big Easy in a Big Way 4
... Mass., March 3 ETEX Corporation, an advanced,biomaterials ... a supply,agreement with LifeNet Health, Inc., Under ... Bone Matrix (DBM) powder to be mixed with ... substitute material called,EquivaBone(TM). ETEX will manufacture EquivaBone(TM) in ...
... Md., March 3 Iomai Corporation,(Nasdaq: IOMI ) ... 2007 financial results before the markets open and hold ... clinical progress,on Thursday, March 6, 2008 at 1:30 p.m. ... the live conference call on Thursday, March 6 at ...
... - BIKAM,pioneers in the discovery and development ... degenerative eye diseases and ocular disorders and,Dalton ... worldwide,collaboration and agreement to discover and develop ... Dalton Medicinal Chemistry will utilize their proprietary ...
Cached Biology Technology:ETEX and LifeNet Health, Inc. Enter Agreement 2Iomai to Announce Fourth Quarter and Year-End 2007 Financial Results and Host Webcast on Thursday, March 6 2BIKAM and Dalton Medicinal Chemistry Sign Collaborative Research Agreement 2
(Date:3/16/2015)... HANOVER, Germany , March 16, 2015 /PRNewswire/ ... DERMALOG Identification Systems GmbH will present groundbreaking innovations ... Hanover, Germany . ... DERMALOG,s latest biometric innovation is well at ... the next generation e-gate made in ...
(Date:3/12/2015)... , March 12, 2015 The mascots ... Brenner Children,s Hospital, part of Wake Forest Baptist Medical ... initiative for the 2015 ACC Men,s Basketball Tournament. Young ... with them and getting their autographs. Brenner ... Baptist. It is the only children,s hospital in northwest ...
(Date:3/12/2015)... Calif. , March 12, 2015 WHEN:Tuesday, ... Complimentary Registration here: http://bit.ly/1G7Os0L . SPEAKERS: , ... Aravind SeshagiriPhoto - http://photos.prnewswire.com/prnh/20150311/181111 ... order to compete in several different markets and ... is currently witnessing an uptrend. ...
Breaking Biology News(10 mins):DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 2DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 3Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 2Biometrics Key to Future Growth in Healthcare, Retail and Financial Markets 3
... drugs of abuse decrease the generation of new neurons ... this process, called neurogenesis, had not been clear. , ... advance of print publication of the November issue of ... from University of Saskatchewan show that a potent and ...
... a type of blue-green algae known as Nostoc, shows promise ... other neurodegenerative diseases, according to an in vitro study by ... time that a potent agent against Alzheimer's has been isolated ... published in the Dec. 26 issue of the Journal ...
... that has been used to track the source of ... may have some new uses: detecting rapidly growing cancers ... known as "stable isotope ratio mass spectrometry," can determine ... or isotopes of an element in the substance ?such ...
Cached Biology News:Natural compound from 'pond scum' shows potential activity against Alzheimer's 2War on terror meets war on cancer 2War on terror meets war on cancer 3War on terror meets war on cancer 4War on terror meets war on cancer 5War on terror meets war on cancer 6
... SAGE (serial analysis of gene ... expression analysis. Expression profiles generated ... to detect low-abundance transcripts making ... genes. The I-SAGE Kit is ...
Sheep Serum US Origin...
... ultimate in utility and flexibility press-to-seal silicone ... needs of the researcher. They are available ... as sheet material that can be easily ... are autoclavable and non-cytotoxic and can be ...
2,4-Dinitrophenyl hapten is conjugated to Human Serum Albumin (HSA) protein through lysine....
Biology Products: