Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, leading to a profile of the expressed genes.1 SAGE provides an accurate quantitative analysis of the relative levels of genes expressed in a specimen. The ability to count many thousands of genes allows the detection of genes that are expressed at very low levels in a high-throughput manner. A three-step molecular approach, the SAGE process allows simultaneous analyses of sequences derived from various cell populations or tissues.2

Introduction

In 1995, Victor Velculescu first described SAGE, a pioneering approach to gene expression analysis that enabled a detailed characterization of expression patterns without prior knowledge of previously isolated genes. The method was originally developed to investigate genes that might be differentially expressed in colon cancer.4

Cancer research remains the focus of the vast majority of SAGE studies. The identification of novel markers for cancer progression and prognosis as in the case of uterine cancer, in which early stage I detection can be difficultis critical for successful treatment. The SAGE method can identify potential targets for therapeutic intervention and find key biochemical pathways. This application is well suited for gene expression analysis of any tissue from any organism.

SAGE is based on the principle that once isolated from a defined position within a transcripta sequence tag of 9 or 10 base pairs (bp) provides sufficient information to identify a transcript. These tags can be serially analyzed by ligating or concatenating them to form longer chains. After they are sequenced, software
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:8/28/2015)... DUBLIN , Aug. 28, 2015 ... announced the addition of the "Global & USA ... offering. The highly awaited US Biosimilar market ... highly awaited filigrastim biologic, Zarxio. How will the global ... The author reveals that biosimilars could save the US ...
(Date:8/28/2015)... - RepliCel Life Sciences Inc. (OTCQB: REPCF) (TSXV: ... the development of autologous cell therapies, today reported ... 2015, corporate highlights, and near-term milestones.  The company,s ... www.sedar.com ,  www.edgar.com and at ... to launch its RCS-01 treatment for aging and ...
(Date:8/27/2015)... ... ... Capital Edge Consulting , a nationwide consulting firm that specializes in helping ... one of the top 5000 companies listed on the 2015 Inc. 5000 , ... is truly an honor to be recognized as one of America’s fastest growing companies. ...
(Date:8/27/2015)... , Aug. 27, 2015  Asterias Biotherapeutics, Inc. (NYSE ... field of regenerative medicine, today announced that executives from ... the month of September.  , ... Rodman & Renshaw,s 17th Annual Global Investment Conference on ... the St. Regis Hotel in New York ...
Breaking Biology Technology:Global & USA BioSimilar Market Analysis Report 2015-2021 2Global & USA BioSimilar Market Analysis Report 2015-2021 3RepliCel Reports Second Quarter 2015 Financial Results 2RepliCel Reports Second Quarter 2015 Financial Results 3RepliCel Reports Second Quarter 2015 Financial Results 4RepliCel Reports Second Quarter 2015 Financial Results 5Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3Asterias Biotherapeutics to Present at Upcoming Investor Conferences 2
... ... ... ... ...
... ... ... ... ...
... ... ... ... ...
Cached Biology Technology:China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 2China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 3China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 4China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 5China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 6China-Biotics, Inc. Appointed Grayling as Strategic Investor Relations Advisor 7Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 2Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 3Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 4Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 5Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 6Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 7Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 8Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 9Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 10Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 11Funds Boost Research into Causes, Interventions for Age-Related Cognitive Decline 12Doctors and First Responders Rely on Oberthur Technologies in Times of Emergency 2Doctors and First Responders Rely on Oberthur Technologies in Times of Emergency 3Doctors and First Responders Rely on Oberthur Technologies in Times of Emergency 4Doctors and First Responders Rely on Oberthur Technologies in Times of Emergency 5
(Date:8/6/2015)... , Aug. 6, 2015 Synaptics ... of human interface solutions, today announced collaboration with ... on Windows 10. Microsoft leveraged Synaptics, deep expertise ... operating system. Through stringent testing, ... fully certified with Microsoft,s Precision TouchPad (PTP) specification ...
(Date:8/5/2015)... , August 5, 2015 Transparency ... "Facial Recognition Market - Global Industry Analysis, Size, Share, ... market for facial recognition is forecast to reach US$ ... increased demand for surveillance systems by civil and government ... of crimes and terrorist activities across the globe that ...
(Date:8/4/2015)... 04, 2015 ... announced the addition of the "Digital ... Service), Sub-Segment (Computer Forensics, Network Forensics, Mobile ... Service, Vertical and Region - Global Forecast ... By Component (Hardware, Software, and ...
Breaking Biology News(10 mins):Synaptics Chosen for Human Interface Co-Development on Windows 10 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 2Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 3Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 4Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 5Facial Recognition Market Expected to Reach US$ 2.67 Bn by 2022, at a CAGR of 9.5% From 2015 - 2022: Transparency Market Research 6Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2
... WEST LAFAYETTE, Ind. - Honeybee populations have been ... may have identified one of the factors that cause ... found dead in and around hives from several apiaries ... neonicotinoid insecticides, which are commonly used to coat corn ...
... of Oviedo (Spain) have come up with a way of tagging ... it has been detonated. Based on the addition of isotopes, the ... fish and those from a fish farm, such as trout and ... and living beings has just been presented in this month,s issue ...
... experts at the University of California, Santa Cruz and ... of seven advanced robotic surgery systems for use by ... a round of final tests, five of the systems ... University, Johns Hopkins University, University of Nebraska, UC Berkeley, ...
Cached Biology News:Researchers: Honeybee deaths linked to seed insecticide exposure 2Researchers: Honeybee deaths linked to seed insecticide exposure 3Explosives and fish are traced with chemical tags 2'Open-source' robotic surgery platform going to top medical research labs 2
Mouse Activin RIB/ALK-4 MAb (Clone 207304)...
... 19 x 29 cm, 1. One ... staining of Coomassie Blue or silver-stain gels ... blots for high sensitivity ECL detection and ... volume, and processing time.Proven design for consistent, ...
Cytidine 5'-Triphosphate, Sodium (CTP), 1 g. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
... Reagent Pack, 1. Contains reagents for ... mouse and rabbit primary antibodies. Designed for ... reagents are not included, but the system ... Category: Blotting & Labeling & Detection, ...
Biology Products: