Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, leading to a profile of the expressed genes.1 SAGE provides an accurate quantitative analysis of the relative levels of genes expressed in a specimen. The ability to count many thousands of genes allows the detection of genes that are expressed at very low levels in a high-throughput manner. A three-step molecular approach, the SAGE process allows simultaneous analyses of sequences derived from various cell populations or tissues.2

Introduction

In 1995, Victor Velculescu first described SAGE, a pioneering approach to gene expression analysis that enabled a detailed characterization of expression patterns without prior knowledge of previously isolated genes. The method was originally developed to investigate genes that might be differentially expressed in colon cancer.4

Cancer research remains the focus of the vast majority of SAGE studies. The identification of novel markers for cancer progression and prognosis as in the case of uterine cancer, in which early stage I detection can be difficultis critical for successful treatment. The SAGE method can identify potential targets for therapeutic intervention and find key biochemical pathways. This application is well suited for gene expression analysis of any tissue from any organism.

SAGE is based on the principle that once isolated from a defined position within a transcripta sequence tag of 9 or 10 base pairs (bp) provides sufficient information to identify a transcript. These tags can be serially analyzed by ligating or concatenating them to form longer chains. After they are sequenced, software
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:10/22/2014)... Hill, SC (PRWEB) October 22, 2014 ... expansion of its cardiovascular pharmacogenetics menu, which enables ... patient outcomes. With PCLS’s evidence-based results, healthcare ... needs and optimize their therapy, while minimizing risks ... In the U.S., according to the FDA [1] ...
(Date:10/22/2014)... -- Research and Markets  has announced the addition ... Application, Geography - Global Analysis and Forecast (2014 - ... Organic electronics, also called as polymer electronics or plastic ... with small conductive molecules and electrically conductive polymers. It ... are carbon based, made using synthetic strategies developed in ...
(Date:10/20/2014)... Calif. , Oct. 20, 2014 ... that the Company has signed a Notice of ... Regenerative Medicine (CIRM), effective October 1, 2014.  The ... Development payments and the release of additional grant ... CIRM grant award for clinical development of Asterias, ...
(Date:10/20/2014)... PLAINSBORO, N.J. (PRWEB) October 20, 2014 ... that the Sidney Kimmel Cancer Center at Thomas ... program. , Through the Strategic Alliance Partnership ... and OncLive will collaborate to raise awareness of ... cancer treatment, and other projects. Clinicians and other ...
Breaking Biology Technology:PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3
... Fusion2006 CEO-CIO Symposium , WTN caught up with Tom Koulopoulos for ... good idea. Read part one . , , WTN: You ... of development to other organizations. But doesn't outsourcing also introduce risk, ... Koulopoulos: Probably the second most likely area of concern ...
... us, media companies do not have the luxury of being ... of Professional Journalists. , ,Speaking Wednesday on the future ... said newspapers and other media outlets already have ceded too ... cannot afford to wait as other industries quickly move to ...
... a growing body of shareholder litigation may cost a ... that members of its management team violated federal securities ... developer of medical imaging and information management software, have ... lawsuits stem from Merge's $325 million stock purchase of ...
Cached Biology Technology:IT outsourcing according to Tom Koulopoulos, part 2 2IT outsourcing according to Tom Koulopoulos, part 2 3IT outsourcing according to Tom Koulopoulos, part 2 4Journalist warns against late arrival to the tech party 2Merge's merger is the crux of shareholder lawsuits 2
(Date:10/14/2014)... prostate cancer tumors scavenge and hoard copper that is ... may be a fatal weakness. , Researchers at ... cancer cells by delivering a trove of copper along ... brimming with the mineral, leaving non-cancer cells healthy. , ... available for other uses, could soon be tested in ...
(Date:10/14/2014)... Ohio (Oct. 14, 2014)—It,s been millions of years since ... a team led by Ohio University scientists is breathing ... model airflow through dinosaur snouts. The research has important ... only breathe but to enhance the sense of smell ... ,nosy, animals," said Ohio University doctoral student Jason Bourke, ...
(Date:10/14/2014)... of the planet,s leading questions is how to produce ... variable climate. The Food and Agriculture Organization of the ... over the next 40 years to feed a growing ... the necessary rise in food production. Plants—grains, cereals, ... supporting livestock. Current research must tap into our ...
Breaking Biology News(10 mins):Prostate cancer's penchant for copper may be a fatal flaw 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
... , College Park, MD (July 20, 2010) -- Recently, ... unusual quantum mechanical effects in the most ubiquitous of ... light into chemical energy. In particular, physicists have suggested ... objects like photons, electrons, or atoms that are separated ...
... validation of the discovery of the world,s first mass ... from natural clay, would be a safer, more environmentally ... plastics nanocomposites. A report on the research appears in ... Rafailovich and colleagues focused on a new organoclay developed ...
... th Annual Meeting, Neuroscience 2010, is the world,s largest ... offers unparalleled access to the latest research innovations and the ... several public events will be held on topics from ... and announced in coming weeks. For more details ...
Cached Biology News:Media registration opens for Neuroscience 2010 2
... enclosures are designed for ... other process gas to ... HEPA filtration, heating/cooling, humidification, ... also available, along with ...
... Staccato Series of systems provide fast and ... , genomics, proteomics and drug development laboratories. ... configurations; Mini Workstation Series, Application Series and ... Station is a remarkably compact system for ...
... ability to quickly and accurately copy or reformat ... candidate for inclusion in these high speed stores. ... all the Cartesian products to allow integration into ... copy plates in seconds or reformat low density ...
... Low-noise, low-vibration filter/fan unit provides continuous vertical ... Class 100 (ISO 5) cleanliness standards ... to clean and disinfect , ... open to allow insertion of filling machines ...
Biology Products: