Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, leading to a profile of the expressed genes.1 SAGE provides an accurate quantitative analysis of the relative levels of genes expressed in a specimen. The ability to count many thousands of genes allows the detection of genes that are expressed at very low levels in a high-throughput manner. A three-step molecular approach, the SAGE process allows simultaneous analyses of sequences derived from various cell populations or tissues.2

Introduction

In 1995, Victor Velculescu first described SAGE, a pioneering approach to gene expression analysis that enabled a detailed characterization of expression patterns without prior knowledge of previously isolated genes. The method was originally developed to investigate genes that might be differentially expressed in colon cancer.4

Cancer research remains the focus of the vast majority of SAGE studies. The identification of novel markers for cancer progression and prognosis as in the case of uterine cancer, in which early stage I detection can be difficultis critical for successful treatment. The SAGE method can identify potential targets for therapeutic intervention and find key biochemical pathways. This application is well suited for gene expression analysis of any tissue from any organism.

SAGE is based on the principle that once isolated from a defined position within a transcripta sequence tag of 9 or 10 base pairs (bp) provides sufficient information to identify a transcript. These tags can be serially analyzed by ligating or concatenating them to form longer chains. After they are sequenced, software
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:5/21/2013)... , May 21, 2013  On May 9, ... ), the global business advisory firm dedicated to ... (the "Company"), announced that it has combined the ... formerly included within the Corporate Finance/Restructuring segment with ... to form a single integrated practice whose financial ...
(Date:5/21/2013)... 2013  The Lindsley F. Kimball Research Institute of the ... Grand Challenges Explorations winner, an initiative funded by ... Sara Lustigman , Head of the Laboratory of ... research project, titled Innovative 3-D in vitro ... to screen drugs to help eliminate onchocerciasis (river blindness) in ...
(Date:5/21/2013)... 21, 2013 Custom Learning Designs, ... eLearning solution using Tin Can API combined with ... CLD is a leading developer of training content ... companies worldwide. , Tin Can API is ... learners and trainers more accurately measure the Return ...
(Date:5/21/2013)... WA (PRWEB) May 21, 2013 Through ... trainings, and funds for lab equipment, 25 organizations will ... and importance of optics and photonics in daily life ... . , The organizations, located in 10 countries, are ... to be awarded by SPIE, the international society ...
Breaking Biology Technology:FTI Consulting Releases Realigned Segment Information Reflecting Newly Combined Health Solutions Practice 2New York Blood Center's Research Institute Receives Grand Challenges Explorations Grant For Groundbreaking Research in Global Health and Development 2New York Blood Center's Research Institute Receives Grand Challenges Explorations Grant For Groundbreaking Research in Global Health and Development 3CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 3Learning About Light: Education Outreach Projects Funded in 10 Countries Through First Round of SPIE Grants 2Learning About Light: Education Outreach Projects Funded in 10 Countries Through First Round of SPIE Grants 3
... The Biomedical Technology Alliance, a group of investors, scientists ... annual report that it is creating a roadmap for ... , ,Founded in 2004, the alliance received $500,000 from ... That was not quite the $2.5 million in state ...
... in vogue these days, thanks to record oil prices and ... by producing more energy at home. While the debate continues ... such as corn, a quiet revolution is well under way ... ,About 8.5 million farmers in 21 countries grew engineered crops ...
... another national mention as a "biotech hotspot," this time ... recognition raises the state's profile and is just what ... Wisconsin on the list of five hotspots were California, ... responsible for compiling the list, told Forbes it was ...
Cached Biology Technology:Biotech crops gaining acceptance, but their full promise has yet to be fulfilled 2Biotech crops gaining acceptance, but their full promise has yet to be fulfilled 3
(Date:5/21/2013)... monitoring" device attached to a whale entangled in fishing ... lines changed a whale,s diving and swimming behavior. The ... eat and migrate, depletes their energy as they drag ... a slow death. , The scientists in this entanglement ... a two-year-old female North Atlantic right whale called Eg ...
(Date:5/21/2013)... being considered in the management of natural resources, ... can help them recognize and respond to the ... lives. , Place-Based Conservation: Perspectives from the ... Pacific Northwest Research Station,s Linda Kruger, can help ... book is published by Springer Verlag and is ...
(Date:5/21/2013)... Wash. A new analysis shows that the nation,s ... of enough algae to produce up to 25 billion ... States, one-twelfth of the country,s yearly needs. , The ... resources that would be needed to grow significant amounts ... results were published in the May 7 issue of ...
Breaking Biology News(10 mins):Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 2Going green: Nation equipped to grow serious amounts of pond scum for fuel 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 4
... farmers have used the plant hormone auxin for decades, but ... mystery. Now researchers at the University of California, San ... agriculture. , The study, published in the July ... discovery of a whole family of auxin genes, and shows ...
... ground-breaking treatment for nerve damage caused by diabetes has ... , The University of Manchester team has discovered that ... a person's genes may prevent nerve damage ?primarily to ... The positive preclinical results ?reported in the journal Diabetes ...
... team of scientists led by Professor Nick Dixon at the ... cracked one of the great DNA mysteries. For more than ... last step in the copying of DNA in cells that ... in the prestigious international journal Cell this morning. , In ...
Cached Biology News:UC San Diego biologists solve plant growth hormone enigma 2UC San Diego biologists solve plant growth hormone enigma 3Trial success for diabetic nerve therapy 2ANU scientists crack DNA replication mystery 2
Mouse monoclonal [TONI-1] to Angiotensin II Type 1 Receptor (rating: ****) ( Abpromise for all tested applications). entrezGeneID: 185 SwissProtID: P30556...
TGF-beta 1,-2,-3 MAb (Clone 1D11)...
HSV-1 gE Envelope Protein (9H3)...
... Stem cells are functionally defined by ... ability to differentiate into multiple specialized progenitor ... along specific lineages. Neural stem cells (NSCs) ... types including astrocytes, neurons and oligodendrocytes. During ...
Biology Products: