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Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

could produce improvements in both yield and throughput.

The use of IF/THEN method control and dialog boxes by the method developer provides the end-user with an environment where all instructions for running the method can be contained within the runtime environment, eliminating the requirement for a paper protocol. Although the improved reliability of automated pipetting is the primary benefit for automating these kits, by using a common layout between each of the kits, greater walk-a-way times are created, with less user intervention required. Furthermore, with the addition of a plate transport and storage device, a fully automated solution could easily be built.

1 Lizardi, P. et. al. 1998. Nat. Genet. 19 pp. 225-232.
2 Nelson J.R. et. al. 2002. Genomics. 80 (6) pp. 691-698.
3 Nelson J.R. et. al. 2002. Biotechniques. June Suppl. pp. 44-47.


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