Navigation Links
Real-Time PCR: General Considerations, Rev A

s clearly depicted in Figure 3. The threshold cycle was shifted by 4.7 cycles at 104 copies and by 3.6 cycles at 107 copies when the MgCl2 concentration was increased from 1.5 mM to 3.0 mM. This significant shift in CT was not observed in the 2.25 mM or 4 mM MgCl2 samples.

PCR efficiency can be affected by numerous factors. In this technical note we have evaluated 2 of these factors secondary structure interference and free magnesium ion concentration. We have clearly demonstrated that optimization of PCR design and reaction conditions on this template has strong effects on the quality of real-time PCR assays.

Once the primer locations and the MgCl2 concentrations have been evaluated, other reaction components, such as probe concentration and protocol temperatures, may need to be evaluated. For example, a 2-step or 3-step PCR thermal protocol may yield optimal amplification detection, depending on the detection strategy. Although this initial optimization may seem cumbersome, it will result in reaction conditions that are robust and reproducible.

* SYBR is a trademark of Molecular Probes, Inc. Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from PE Corporation. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.


back to top
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
10. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
11. General Notes on Primer Design in PCR*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Real Time PCR General Considerations Rev

(Date:9/3/2015)... ... September 03, 2015 , ... UCLA wide receiver and Canadian ... and therapy were not working so Zach decided to undergo stem cell therapy ... with precisely guided injections of bone marrow aspirate concentrate (BMAC) harvested with the ...
(Date:9/3/2015)... 3, 2015 According to a ... (LC (HPLC, UHPLC, Flash), GC, Other Components (Autosamplers, Detectors, ... Vials) - Analysis & Global Forecasts to 2020", published ... reach around 9.223 Billion by 2020 from USD 7.062 ... Browse 141 tables and 36 figures spread through ...
(Date:9/2/2015)... 2015 Research and Markets ( ... Jain PharmaBiotech,s new report "Nanobiotechnology Applications, ... An increasing use of ... is anticipated. Nanotechnology will be applied at ... formulations for optimal delivery to diagnostic applications ...
(Date:9/2/2015)... -- Research and Markets ( ... PharmaBiotech,s new report "Biomarkers - Technologies, Markets ... This report follows the broad definition of a ... measured and evaluated as an indicator of normal ... responses to a therapeutic intervention. Tests based on ...
Breaking Biology Technology:UCLA Wide Receiver and Canadian Decathlon Standout Zack Bornstein Bounces Back After Stem Cell Therapy at Riordan-McKenna Institute 2UCLA Wide Receiver and Canadian Decathlon Standout Zack Bornstein Bounces Back After Stem Cell Therapy at Riordan-McKenna Institute 3Chromatography Instruments Market Worth $ 9.223 Billion by 2020 2Chromatography Instruments Market Worth $ 9.223 Billion by 2020 3Chromatography Instruments Market Worth $ 9.223 Billion by 2020 4Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 2Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 3Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 2Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 3
... 2012  MiMedx Group, Inc. (OTCBB: MDXG), an integrated developer, ... bioimplants processed from human amniotic membrane, announced today that ... the first quarter of 2012. The Company,s revenue goal ... The Company also reported that several large ...
... shown that prospective magnetic fusion power systems would pose ... production of weapon-usable materials than nuclear fission reactors and ... Princeton University, found that if nuclear fusion power plants ... risk of fissile materials being produced for weapons, either ...
... are one of the core technologies of the computing industry, ... memory that are faster, smaller, and more energy efficient than ... tool developed by a team from the National Institute of ... the Royal Institute of Technology in Swedena method to detect ...
Cached Biology Technology:MiMedx Exceeds Revenue Goal for First Quarter of 2012 2Fusion presents low proliferation risk, experts conclude 2Nanoscale magnetic media diagnostics by rippling spin waves 2
(Date:8/6/2015)... Germany , August 6, 2015 /PRNewswire/ ... SensoMotoric Instruments (SMI) shows the world,s first ... based on Epson,s Moverio BT-200 see-through head mounted display ... new solution, unprecedented quality and efficiency is brought to ... displays. For the first time, professionals and researchers can ...
(Date:8/5/2015)... 5, 2015 According to a ... Market by Software, Services, Vertical (BFSI, Airport, IT & Telecom, ... Global Forecast to 2020", published by MarketsandMarkets, defines and ... into various segments. The global PIAM Market is estimated ... Million by 2019, at a CAGR of 14.9 % ...
(Date:8/5/2015)... , Aug. 5, 2015 The biosensors ... growth in applications, penetration into newer sectors, and development ... The global biosensors space has seen the entry of ... market so far. (Photo - http://photos.prnewswire.com/prnh/20150805/255570 ... Sullivan, Analysis of the Global Biosensors Market ...
Breaking Biology News(10 mins):SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4
... study published in the latest issue of Ethology researchers show ... possibly the sex of their chicks according to how they ... attractive ("sexy") songs and less attractive control songs of male ... they varied the size of their eggs in the nest ...
... published in The Journal of Physiology suggests that a ... by unexpected loud sounds. , Whiplash injuries most commonly ... for the injury is not known but it is ... relative to the head damages the joints and muscles ...
... University study is adding credence to an increasingly popular ... bone marrow cells to regenerate organ tissue. , Scientists ... cells derived from adult bone marrow can fuse with ... comprising the cellular lining of intestinal walls, known as ...
Cached Biology News:OHSU study says stem cell 'fusion' occurs in tumors 2OHSU study says stem cell 'fusion' occurs in tumors 3
Mouse VEGF R1/Flt-1 Phycoerythrin MAb (Clone 141522) Keywords: Receptor Tyrosine Kinase, RTK, VEGFR1, VEGF Receptor 1 Protein Family: Receptor Tyrosine Kinases, VEGF/PDGF Family...
Recognizes Kir4.1. The epitope does not share homology with any other known proteins.,SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Oth...
... ELISA Development kit contains the basic components ... to measure natural and recombinant mouse Dickkopf ... serum. Each kit contains sufficient materials to ... provided that the following conditions are met: ...
HIV-1 TAT (vN-21)...
Biology Products: