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Real-Time Immuno-PCR on the iCycler iQ System, Rev A

s common to standard ELISA and can be reduced by proper blocking of the surface or by binding the capture antibody more tightly to allow for more extensive washing. These approaches are being developed.

The results presented here and also those reported previously (Sims et al. 2000) are promising indications that applications of real-time PCR will expand to protein detection. A challenging future task will then be to quantitate the levels of both mRNA and the corresponding protein in only a few cells, or perhaps even a single cell, to study the correlation between gene and protein expression.


Acknowledgements
We thank CanAg Diagnostics for supplying antibodies, antigens, and buffers. We also thank our colleagues at the TATAA Biocenter for valuable discussions.


References
CanAg PSA EIA Instructions, Product No. 300-10, June 2000. Available from CanAg Diagnostics, www.canag.se

Sano T et al., Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates, Science 258, 120122 (1992)

Sims PW et al., Immunopolymerase chain reaction using real-time polymerase chain reaction for detection, Anal Biochem 281, 230232 (2000)


Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applied Biosystems. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

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