Navigation Links
Real-Time Immuno-PCR on the iCycler iQ System, Rev A

s common to standard ELISA and can be reduced by proper blocking of the surface or by binding the capture antibody more tightly to allow for more extensive washing. These approaches are being developed.

The results presented here and also those reported previously (Sims et al. 2000) are promising indications that applications of real-time PCR will expand to protein detection. A challenging future task will then be to quantitate the levels of both mRNA and the corresponding protein in only a few cells, or perhaps even a single cell, to study the correlation between gene and protein expression.


Acknowledgements
We thank CanAg Diagnostics for supplying antibodies, antigens, and buffers. We also thank our colleagues at the TATAA Biocenter for valuable discussions.


References
CanAg PSA EIA Instructions, Product No. 300-10, June 2000. Available from CanAg Diagnostics, www.canag.se

Sano T et al., Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates, Science 258, 120122 (1992)

Sims PW et al., Immunopolymerase chain reaction using real-time polymerase chain reaction for detection, Anal Biochem 281, 230232 (2000)


Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applied Biosystems. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

Germall is a trademark of ISP Chemicals, Inc. JumpStar
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
10. Real-Time PCR: General Considerations, Rev A
11. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Real Time Immuno PCR the iCycler System Rev

(Date:10/30/2014)... 30, 2014 A new report, commissioned by ... takes an in-depth look at Indiana,s ... resulted over two different time frames: 1993 to 2002 ... of future opportunities for investment growth.  The Life ... report shows gains in innovation dollars as well as ...
(Date:10/30/2014)... 2014) – The severe flooding that devastated a wide ... destructive if the bridges, roads and other infrastructure had ... from the University of Colorado Denver. , "People need ... Jimmy Kim, PhD, associate professor of structural engineering at ... and lead author the study. "There is an assumption ...
(Date:10/27/2014)... -- The Italian company Roadrunnerfoot has ... its artificial "lower limb", after the German giant ...      (Photo: http://photos.prnewswire.com/prnh/20141027/713097 ) , ... judgement has arrived: Roadrunnerfoot, the small Italian company ... prostheses with composite materials, has won against global ...
(Date:10/27/2014)... 2014 The “Molecular Diagnostic Market ... Technology (PCR, INAAT, DNA Sequencing), End User (Hospital, ... - Global Forecast to 2018” provides a detailed ... current market trends, and strategies impacting the global ... forecasts of the revenue and share analysis. , ...
Breaking Biology Technology:New report shows Indiana's gains in life sciences innovation capital outpace the nation 2New report shows Indiana's gains in life sciences innovation capital outpace the nation 3New report shows Indiana's gains in life sciences innovation capital outpace the nation 4New report shows Indiana's gains in life sciences innovation capital outpace the nation 5CU Denver study says upgrading infrastructure could reduce flood damage 2Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 2Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 3Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 4Global Molecular Diagnostics Market Will Reach $7.9 Billion by 2018 - New Research Report by MarketsandMarkets 5
... DIEGO, June 14, 2011 OncoSec Medical Inc. ... therapies to treat solid tumor cancers, announced today ... lead the clinical operations of OncoSec,s ElectroOncology therapies. ... Punit Dhillon, OncoSec,s President and CEO, said, "OncoSec ...
... Fox Chase Cancer Center , one of ... announced it has entered into an agreement with Life ... provide next-generation sequencing analysis of solid tumors.  The agreement ... Institute at Fox Chase, a facility that will foster ...
... Dendreon Corporation (Nasdaq: DNDN ) today announced that ... the Ernst & Young Entrepreneur Of The Year ® ... category. Dr. Gold has served as CEO ... last year received U.S. Food and Drug Administration approval for ...
Cached Biology Technology:OncoSec Announces Clinical Leadership Appointments 2OncoSec Announces Clinical Leadership Appointments 3OncoSec Announces Clinical Leadership Appointments 4Fox Chase Cancer Center Signs Agreement with Life Technologies Corporation 2Fox Chase Cancer Center Signs Agreement with Life Technologies Corporation 3Fox Chase Cancer Center Signs Agreement with Life Technologies Corporation 4Fox Chase Cancer Center Signs Agreement with Life Technologies Corporation 5Dendreon CEO Dr. Mitchell Gold Named Ernst & Young Entrepreneur Of The Year® 2011 Pacific Northwest Award Winner 2Dendreon CEO Dr. Mitchell Gold Named Ernst & Young Entrepreneur Of The Year® 2011 Pacific Northwest Award Winner 3Dendreon CEO Dr. Mitchell Gold Named Ernst & Young Entrepreneur Of The Year® 2011 Pacific Northwest Award Winner 4Dendreon CEO Dr. Mitchell Gold Named Ernst & Young Entrepreneur Of The Year® 2011 Pacific Northwest Award Winner 5
(Date:10/29/2014)... is open for the Society of Interventional ... 2015 is the one place where interventional ... health care professionals can come together to ... technologies being presented, discussed and tested. ... invasive, image-guided medicine, SIR 2015 will feature ...
(Date:10/29/2014)... chemicals from household and industrial products are in the ... for most of them, scientists have yet to determine ... first step toward doing that by estimating which substances ... is published in the ACS journal Environmental Science ... note that the risks to human health of any ...
(Date:10/29/2014)... LANSING, Mich. – As bodies decompose, their types ... the clues they provide could mean the difference ... , Michigan State University is using a more ... help Detroit death-scene investigators examine these changing populations. ... as geographical location of death, gender, race, socioeconomic ...
Breaking Biology News(10 mins):SIR 2015: Interventional radiology shapes tomorrow's medicine 2MSU partners with Detroit to investigate death scenes 2
... let the bedbugs bite" is becoming an impossible dream ... resurgence of an ancient scourge that is fostering human ... potentially toxic materials. That,s the message from the cover ... News (C&EN), ACS, weekly newsmagazine. In the article, ...
... world leading publisher of scientific information products and services, ... Environment, today formalized their collaboration to publish the new ... the final quarter of 2011. This publication, supported by ... to the dynamic and impressive expansion of both environmental ...
... whale season, researchers from Hawai,i Institute of Marine Biology ... shed new light on the wintering grounds of the ... Pacific was always thought to be the main Hawaiian ... these grounds extend all the way throughout the Hawaiian ...
Cached Biology News:SCOPE and Elsevier partner to publish Environmental Development 2Researchers discover new wintering grounds for humpback whales using sound 2
... The PinPoint™ Vector Sequencing Primer is ... PinPoint™ Xa Vectors (Cat.# V2031, V2051, V2061). ... Xa site at nucleotides 325343, approximately 4050 ... region and can be used to determine ...
Medium for cryopreserving subcutaneous or omental preadipocytes...
... ultra high yield technology is fast - ... aRNA for gene array analysis ... , Kits specific for templates with ... , All kits contain SUPERaseIn ...
Rad 51C Immunogen: His-tagged human Rad 51C, over-expressed in E. coli Storage: 4 C...
Biology Products: