Navigation Links
Real-Time Immuno-PCR on the iCycler iQ System, Rev A

g/L with phosphate buffer containing BSA and immunoglobulins to reduce nonspecific adsorption (CanAg PSA EIA instructions).

To each well, 25 l of the diluted biotinylated anti-PSA66 was added and the samples were incubated for 1 hr at room temperature. After washing six times with wash buffer 1, the wells were incubated for 30 min at room temperature with 25 l streptavidin solution (0.3 g/ml streptavidin in 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 2.5 mM MgCl2). Thereafter, the samples were washed six times with wash buffer 1 and incubated with 25 l of biotinylated DNA marker (15 pM). The wells were then washed six times with wash buffer 1, followed by four times with wash buffer 2 (0.154 M NaCl, 5 mM Tris, pH 7.75), and then left to stand in wash buffer 2 for 1 hr before removing the buffer. Finally, the PCR mix was added to the wells and real-time PCR was performed in the iCycler iQ system (Figure 2). Approximately 1520 cycles were needed to reach threshold, set at 10,000 relative fluorescence units (RFU), which was substantially above the background but still in the exponential growth phase of the PCR. The CT vs. log (concentration) plot was linear with a large correlation coefficient (r = 0.986), showing that the assay correctly and accurately reflects the amount of PSA in the studied range. However, PCR products that appear in negative control samples limit sensitivity. Analysis revealed two sources of these products. One is formation of primer-dimers, which form independently of the presence of template. There are several ways to suppress their formation through assay optimization techniques. The second source of error is nonspecific adsorption of the detection antibody or DNA. This i
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
10. Real-Time PCR: General Considerations, Rev A
11. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Real Time Immuno PCR the iCycler System Rev

(Date:4/1/2015)... NEW YORK CITY and ... leading provider of market research reports and solutions, MarketResearch.com is ... that 2015 will be a year of market growth and ... operating a stem cell company or cord blood bank, competing ... must analyze market conditions, predict industry trends, and act on ...
(Date:4/1/2015)... 1, 2015 Immunocore Limited ... biological drugs to treat cancer, viral infections and ... appointment of Christina Coughlin as Chief ... providing medical and regulatory leadership to the pre-clinical ... Dr. Coughlin has extensive experience in oncology drug ...
(Date:3/31/2015)... (PRWEB) March 31, 2015 Eastern ... schools for online bachelor’s of criminal justice degree ... connecting people to inspiring ideas and high quality ... e-mail to the University. We are confident that ... , This is the site’s first “Students ...
(Date:3/31/2015)... 31, 2015  Bayer HealthCare (Bayer) has expanded ... Institute of Technology (MIT) and Harvard University to ... of this new part of the alliance is ... create new cardiovascular therapies. "We are ... Institute to the area of cardiovascular genomics to ...
Breaking Biology Technology:MarketResearch.com: "Blockbuster" Year for Stem Cells Creates Market Opportunities, Says BioInformant Worldwide Report 2MarketResearch.com: "Blockbuster" Year for Stem Cells Creates Market Opportunities, Says BioInformant Worldwide Report 3MarketResearch.com: "Blockbuster" Year for Stem Cells Creates Market Opportunities, Says BioInformant Worldwide Report 4Immunocore Appoints Dr. Christina Coughlin as Chief Medical Officer 2Immunocore Appoints Dr. Christina Coughlin as Chief Medical Officer 3Immunocore Appoints Dr. Christina Coughlin as Chief Medical Officer 4EKU Ranked in Top 50 Among Online Criminal Justice Degrees 2Bayer HealthCare Expands Collaboration with Broad Institute of MIT and Harvard to Develop Therapies for Cardiovascular Disease 2Bayer HealthCare Expands Collaboration with Broad Institute of MIT and Harvard to Develop Therapies for Cardiovascular Disease 3
(Date:3/12/2015)... Beta Systems today announced ... management (IAM) solution for a fixed price and ... new IAM package, customers benefit from the 30 ... IAM implementations across different industries. The new fixed-price ... any necessary services and consulting. It spans the ...
(Date:3/10/2015)... NEW YORK , March 10, 2015  Continuing ... and the Unexpected, Hammacher Schlemmer introduces The Eye ... user,s identity before granting access to secure websites or ... trusted to verify travelers at international borders, the device ... 240 distinctive pattern points of the iris, converting them ...
(Date:3/5/2015)... In Brazil , cloud computing ... these solutions and 42 percent planning to invest in them ... percent of the companies will be investing in these solutions ... companies in the region are currently opting for the private ... command significant attention in the coming years. This trend is ...
Breaking Biology News(10 mins):Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2Hammacher Schlemmer Introduces The Eye Scanning Password Authenticator 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 3Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 4
... CORVALLIS, Ore. Researchers at Oregon State University have made ... lead toward robots that not only can walk and run ... achieving an optimal approach with robotic mechanisms, studies are moving ... the military, create prosthetic limbs for humans that work much ...
... PHILADELPHIA - A team of paleontologists, including a University ... dinosaur based upon an incomplete skeleton found in western New ... rocks that preserve a swampy forest ecosystem that thrived near ... ago. The dinosaur, whose name translates to mean "grinding-mouth, ...
... considering Denmark has always been praised as the benchmark ... treatment for patients seeking ART, said Dr. Sren Ziebe ... of ESHRE and the SIG coordinator of all SIGs ... assisted reproduction treatments (ART) with up to three treatment ...
Cached Biology News:Advances made in walking, running robots 2Advances made in walking, running robots 3'Grinding mouth, wrinkle eye': Penn graduate student describes new species of plant-eating dinosaur 2
Normal canine serum collected from healthy normal canines....
... fosmid (40kb), and large plasmid insert (10-20kb) ... BAC vector. On command 20-50 fold copy ... DNA purification. Available with blue-white screening (pEZ ... the highest insert stability. Kits include electrocompetent ...
RABBIT ANTI GLYCINE...
... Light Diagnostics Cytomegalovirus (CMV) pp65 Antigenemia Immunofluorescence ... identification of lower matrix protein pp65 of ... Kit Components: Erythrocyte ... bottle containing 220 mL of buffered ammonium ...
Biology Products: