Navigation Links
RNAlater Around the World: The Art of Processing Difficult Samples


Draw on the strength of RNAlater to protect cellular RNA within specimens during sample collection or shipping. Whether you are in the lab or out in the field, this aqueous, non-toxic Tissue Collection:RNA Stabilization Solution functions from ambient temperatures down to -20C. Here is a snapshot of how researchers have used RNAlater in exciting discoveries around the world.


Pancreas--Germany

Isolating intact RNA from pancreas can be difficult because of high levels of nucleases expressed in this tissue. In a study about circadian expression of clock genes, scientists used RNAlater to collect rat pancreata at three hour intervals for eventual quantitative RT-PCR analyses. These experiments provide growing evidence of circadian pacemakers in islets and begin to examine the role of dysrhythmic insulin secretion at the onset of diabetes mellitus. To learn more, see FEBS Lett (2004) 564: 91-96.


Blood--United States

RNAlater can be used to protect RNA in blood samples for any research or diagnostic test. One research group has shown that this may be critical for maintaining gene expression patterns in peripheral blood mononuclear cells (PBMCs). When PBMCs were resuspended in RNAlater within 30 minutes of collection, major changes in gene expression profiles related to ex vivo stress response pathways or to RNA degradation were reduced. To learn more, see Genes Immun (2004) 5: 347-353.


Environmental Bacteria--Netherlands Antilles

To study differential gene expression in bacterial communities associated with coral black band disease (BBD), scuba-diving scientists collected active BBD mat samples from infected coral surfaces and BBD mat samples that had been moved 0.3 to 1 m from the reef. Upon removal from the ocean, their samples were stabilized in RNAlater for eventual RNA-arbitrarily primed PCR (RAP-PCR) and RT-PCR analyses. These experiments are providing important clues to the global problem of BBD migration as well as the infection and destruction of coral reefs by BBD. To learn more, see Appl Environ Microbiol (2004) 70: 3687-94.


Environmental Virus-- Laos People's Democratic Republic

To find ways of improving diagnostic testing of classical swine fever (hog cholera), researchers simulated collection and transport conditions in tropical ambient temperatures for virus-positive spleen samples in RNAlater or a glycerol/saline buffer. In both widely used diagnostic tests (RT-PCR and antigen-capture ELISA), viral detection was more sensitive in samples preserved with RNAlater. To learn more, see J Virol Methods (2004) 118: 33-7.


back to top


Ordering Information
Cat# Product Name Size 7020 RNAlater 100 ml 7021 RNAlater 500 ml 7022 RNAlater 50 x 1.5 ml 7023 RNAlater 20 x 5 ml 7024 RNAlater 250 ml
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
2. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
3. RNAlater FAQs: Preserving RNA Before Isolation
4. Your Data: RNAlater Stories
5. RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater
6. Genomic DNA Preparation from RNAlater Preserved Tissues
7. New Tools for Making and Processing Protein Microarrays.
8. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
9. Delivering siRNAs to Difficult Cell Types
10. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
11. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/10/2017)... , Oct. 10, 2017 International research firm Parks ... Strategy, will speak at the TMA 2017 Annual Meeting , October ... trends in the residential home security market and how smart safety and ... Parks ... "The residential ...
(Date:10/9/2017)... 2017  BioTech Holdings announced today identification and ... ProCell stem cell therapy prevents limb loss in ... demonstrated that treatment with ProCell resulted in more ... compared to standard bone marrow stem cell administration.  ... reduction of therapeutic effect.  ...
(Date:10/9/2017)... Antonio, Texas (PRWEB) , ... ... ... new study published on October 5, 2017, in the medical journal, Epilepsia, ... equivalence with the gold standard, video EEG, in detecting generalized tonic-clonic seizures ...
(Date:10/6/2017)... , ... October 06, 2017 , ... ... within the healthcare and technology sector at their fourth annual Conference where founders, ... 30 inspiring speakers and the ELEVATE pitch competition showcasing early stage digital health ...
Breaking Biology Technology:
(Date:4/3/2017)... , April 3, 2017  Data ... precision engineering platform, detected a statistically significant ... product prior to treatment and objective response ... the potential to predict whether cancer patients ... to treatment, as well as to improve ...
(Date:3/30/2017)... The research team of The Hong Kong Polytechnic University (PolyU) ... ground breaking 3D fingerprint minutiae recovery and matching technology, pushing contactless ... use in identification, crime investigation, immigration control, security of access and ... ... A research team led by Dr Ajay Kumar ...
(Date:3/29/2017)... the health IT company that operates the largest health ... today announced a Series B investment from BlueCross BlueShield ... investment and acquisition accelerates higi,s strategy to create the ... activities through the collection and workflow integration of ambient ... secures data today on behalf of over 36 million ...
Breaking Biology News(10 mins):