.3 mL/min and the injection volume was 5 L.

Detection was performed on the TSQ Quantum mass spectrometer equipped with the ESI source (Thermo Finnigan, San Jose, CA, USA).

The general MS conditions were as follows:
Source: ESI
Ion Polarity: Positive
Spray Voltage: 4600 V
Sheath / Auxiliary Gas: Nitrogen
Sheath Gas Pressure: 75 arb units
Auxiliary Gas Pressure: 25 arb units
Ion Transfer Capillary Temperature: 360 C
Scan Type: SRM
Collision Gas: Argon
Collision Gas Pressure: 1.5 mTorr


Figure 4. Calibration curve for cabergoline in plasma under unit resolution conditions covering 1 x 10⁵ orders of linear dynamic range (50 fg to 5 ng on column), R > 0.999 using 1/x weighted regression.

The cabergoline SRM conditions were as follows:

Parent Mass: 452 m/z
Product Mass: 381 m/z
Scan Width: 0.7 u
Scan Time: 0.50 s
Collision Energy: 19 eV
Q1 Peak Width: 0.70 u FWHM
Q3 Peak Width: 0.70 u FWHM The pergolide SRM conditions were as follows:

Parent Mass: 315 m/z
Product Mass: 208 m/z
Scan Width: 0.7 u
Scan Time: 0.50 s
Collision Energy: 27 eV
Q1 Peak Width: 0.70 u FWHM
Q3 Peak Width: 0.70 u FWHM


Table 1. Precision and accuracy in the LC/ESI/SRM analysis of cabergoline in plasma under unit resolution conditions. n >=5 samples at each calibration level.

Discussion
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