Navigation Links
Pwo DNA Polymerase

erial and if after PCR single DNA molecules are analyzed, PCR artifacts can be a significant problem. Fidelity of DNA polymerization is for instance important for:
  • cloning of PCR products
  • study of allelic polymorphism in individual RNA transcripts (1, 2)
  • characterisation of the allelic stage of single cells (3) or single DNA molecules (4, 5)
  • characterisation of rare mutations in tissue (6)
  • characterisation of a population of cells in culture
Operating parameters
  • Thermal stability: Pwo DNA Polymerase is stable for >2 h at 95C
  • Divalent ion requirement: Mg2+ (standard concentration, 2.00 mM)
  • dNTP concentration: at least 200 M for each dNTP
    Caution: Lower deoxynucleotide concentrations can increase fidelity but can also activate the 3'5' exonuclease proofreading activity, which might degrade prime
Experimental results Key advantages
  • Maximum PCR fidelity, because the proofreading activity of Pwo DNA Polymerase excises misincorporated nucleotides
  • High PCR yield, because the polymerase is very resistant to the high denaturing temperatures used in PCR
  • Simplifies cloning of PCR products because it generates blunt-ended products which can be directly used f
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Pfu DNA Polymerase as a Hot Start Alternative
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
6. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
7. High-Fidelity PCR with a Novel Polymerase Mixture
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
10. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
11. HotStarTaq DNA Polymerase
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Pwo DNA Polymerase

(Date:1/22/2015)... 22, 2015 Shimadzu Scientific Instruments ... multipurpose UV-visible spectrophotometer. Offering wavelength scanning from 190 ... ideal for applications in a variety of industries, ... The user-friendly UV-1280 enables intuitive operation, while the ...
(Date:1/22/2015)...  Transwestern | RBJ today announces the firm brokered a long-term ... a leading biopharmaceutical company, at Two Ledgemont Center in ... Richards , president, and Brian Cohen , senior vice ... building at 95 Hayden Ave. Photo - ...
(Date:1/22/2015)... Protocol Networks brings independent technology consulting ... brand-neutral, independent consultants, Protocol Networks has recently announced its ... the years, his company has attracted several clients in ... as well as others. With the success of these ...
(Date:1/22/2015)... 2015 zFlo Motion , a US ... an exciting product from a new partner. STT-Systems ... the past 15 years, with offerings in the optical-motion capture, ... IMU system (inertial measurement unit), iSen, is opening eyes around ...
Breaking Biology Technology:Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 23D Motion Capture Just Got Easy 2
... LONDON , February 4 GlaxoSmithKline ... return to,sales growth for the first time since 2007. , ... also announced,a reduction in the investment into treatments for pain and depression and ... "Now obviously I don,t like to see anybody leave the organisation ...
... Global ... ... a unique opportunity today to engage, innovate and deliver sustainable solutions to the growing global ... CEO Forum of the Delhi Sustainable Development Summit 2010 in India. , , ...
... , Research Superiority Award to Enable Recruitment of Stellar ... (TcSUH-ARH) , HOUSTON , Feb. 3 ... Award from the Texas Emerging Technology Fund (ETF), announced today by ... The University of Houston ,s Texas Center for Superconductivity (TcSUH) ...
Cached Biology Technology:Sustainability Is A Business Imperative 2UH Superconductivity Center Receives Texas Emerging Technology Fund Award 2UH Superconductivity Center Receives Texas Emerging Technology Fund Award 3UH Superconductivity Center Receives Texas Emerging Technology Fund Award 4UH Superconductivity Center Receives Texas Emerging Technology Fund Award 5UH Superconductivity Center Receives Texas Emerging Technology Fund Award 6UH Superconductivity Center Receives Texas Emerging Technology Fund Award 7
(Date:1/22/2015)... OXFORD, Conn. , Jan. 20, 2015 NXT-ID, Inc. (NASDAQ: ... growing mobile commerce market, reports on the recent success of the Wocket™ ... Wocket smart wallet was named as one of the ... of the "5 Best Products Launched At CES So Far" by Newseveryday.com ...
(Date:1/22/2015)... Research and Markets ( http://www.researchandmarkets.com/research/wxdglm/global_wearable ) ... Wearable Technologies Market and Applications, Opportunities, Industry Analysis, ... 2014-2020" report to their offering. , ... can be worn on the user,s body and ...
(Date:12/22/2014)... Conn. , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile commerce market, ... Apollo Robbins for the 2015 International CES debut ... Apollo Robbins will be at the NXT-ID booth January ...
Breaking Biology News(10 mins):CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3Global Wearable Technologies Market and Applications, Opportunities, Industry Analysis, Segmentation, Size, Growth, Share, Overview, Trends and Forecast 2014-2020 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... to join the pantheon of superstars in the materials ... size, shape and physical identity, vanadium dioxide can now ... researchers with the U.S. Department of Energy (DOE),s Lawrence ... robotic torsional muscle/motor made from vanadium dioxide that for ...
... peer into a tidepool anywhere along the northeastern U.S. coast, ... They,re most likely common Atlantic slipper shells, a species of ... ubiquitous snails, and discovered that how their larvae swim is ... cranny along the coast. And to how the snails may ...
... YORK , Dec. 19, 2013 /PRNewswire-iReach/ -- HGH.us.com, a ... factual information about HGH and anti aging, has ... will include information about the latest anti aging therapies. The ... aging HGH supplements, weight loss trends, diets, and current special ...
Cached Biology News:A micro-muscular breakthrough 2A micro-muscular breakthrough 3Oh, the places you'll go -- if you're an Atlantic slipper shell 2HGH Supplements Information Website HGH.us.com Announces New Discounts on Sytropin and Genf20 Plus 2HGH Supplements Information Website HGH.us.com Announces New Discounts on Sytropin and Genf20 Plus 3
... the translation boosting Cap 1 structure. mScript™ ... 40% to 80% capped using co-transcriptional systems) ... mScript™ also features the Cap 1 structure, ... to 50% more efficient in in vivo ...
...
Request Info...
... pPIC9K and pPIC3.5K vectors carry the kanamycin ... Reagent in Pichia. Spontaneous generation of multiple ... to increased levels of Geneticin Reagent. Pichia ... screened for their level of resistance to ...
Biology Products: