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PCR Grade Water

ows no detectable degradation of the DNA fragments. Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: 5 g of MS2 RNA are incubated with various amouts of Water, PCR Grade in 50 l test buffer at 37C for 1 h. Up to 20 l Water, PCR Grade shows no detectable RNAse activity. Amplification function test: Each lot of Water, PCR Grade is function tested in PCR. Routinely, PCR is performed using DNA for an 0.5 kb fragment. A clear defined band from 0.01 ng DNA should be clearly visible. Notice to purchase

This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.


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