Navigation Links
PCR Grade Water

Cat. No. 3 315 932, 25 ml (25 vials of 1 ml)
Cat. No. 3 315 959, 25 ml (1 vials of 25 ml)
Cat. No. 3 315 843, 100 ml (4 vials of 25 ml)) Description Specially purified, double distilled, deionized, auto-claved water Free of detectable endonucleases, ribonucleases and nicking activity Routinely function checked in PCR pH at 20C: 8.3 Application This product is especially tested and qualified for use in all PCR or RT-PCR reactions, in conjunction with conventional thermocyclers or with the LightCycler Instrument available from Roche Applied Science. All LightCycler Kits available from Roche Applied Sci-ence and some of the Amplification Products available from Roche Applied Science contain this Water, PCR Grade. Storage and Stability Store a working solution for a short period of time
(e.g. 1 week) at 2 - 8 C Store stock solutions for a longer period of time
at 15 to 25 C Quality Control Test buffer: 10 mM Tris/HCl, pH 7.5 (37C), 10 mM MgCl 2 , 1 mM DTE Absence of endonucleases: 1 g DNA is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable degradation of the DNA. Absence of endonucleases: 1 g DNA, cleaved by Eco RI and Hind III (MWM III) is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade sh ows no detectable degradation of the DNA fragments. Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: 5 g of MS2 RNA are incubated with various amouts of Water, PCR Grade in 50 l test buffer at 37C for 1 h. Up to 20 l Water, PCR Grade shows no detectable RNAse activity. Amplification function test: Each lot of Water, PCR Grade is function tested in PCR. Routinely, PCR is performed using DNA for an 0.5 kb fragment. A clear defined band from 0.01 ng DNA should be clearly visible. Notice to purchase

This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. PCR Grade Deoxynucleotides
2. Enjoy a Greater Sense of Security with PCR-Grade Water
3. PCR Grade Deoxynucleotides Additional power to perfect your PCR experiments
4. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Which Water to Use?
9. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
10. Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/26/2016)... 2016  Agriculture nutrients are in the news these ... is running their nitrate removal system on overtime ... and coastal regions nationwide are painting the water ... issue. NECi Superior Enzymes, a biotechnology company ... a new, easy to use device that fits in ...
(Date:5/26/2016)... YORK , May 26, 2016 ... investors see value in this space. Today,s pre-market research on ... equities: Radius Health Inc. (NASDAQ: RDUS ), Cerus ... ARWR ), and Five Prime Therapeutics Inc. (NASDAQ: ... stock technical briefings at: http://www.activewallst.com/ ...
(Date:5/26/2016)... ... May 26, 2016 , ... FireflySci has been ... leading laboratories all over the globe. Their cute firefly logo has been spreading ... cuvettes, FireflySci makes spectrophotometer calibration standards that never require recalibration. These revolutionary ...
(Date:5/25/2016)... ... May 25, 2016 , ... The ... fracture-specific plating options designed to address fractures of the distal tibia and fibula. ... The Acumed Ankle Plating System 3 is composed of seven plate families that ...
Breaking Biology Technology:
(Date:3/22/2016)... , PROVO and ... -- Newborn Screening Ontario (NSO), which operates the highest ... for molecular testing, and Tute Genomics and UNIConnect, leaders ... technology respectively, today announced the launch of a project ... sequencing (NGS) testing panel. NSO has ...
(Date:3/17/2016)... 17, 2016 ABI Research, the leader ... global biometrics market will reach more than $30 ... from 2015. Consumer electronics, particularly smartphones, continue to ... anticipated to reach two billion shipments by 2021 ... Pavlakis , Research Analyst at ABI Research. "Surveillance ...
(Date:3/14/2016)... Florida , March 14, 2016 ... the growing mobile commerce market, announces the airing of a ... channels starting the week of March 21 st .  The ... CNBC, including its popular Squawk on the Street show. ... focused on the growing mobile commerce market, announces the airing ...
Breaking Biology News(10 mins):