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PCR Grade Water

Cat. No. 3 315 932, 25 ml (25 vials of 1 ml)
Cat. No. 3 315 959, 25 ml (1 vials of 25 ml)
Cat. No. 3 315 843, 100 ml (4 vials of 25 ml)) Description Specially purified, double distilled, deionized, auto-claved water Free of detectable endonucleases, ribonucleases and nicking activity Routinely function checked in PCR pH at 20C: 8.3 Application This product is especially tested and qualified for use in all PCR or RT-PCR reactions, in conjunction with conventional thermocyclers or with the LightCycler Instrument available from Roche Applied Science. All LightCycler Kits available from Roche Applied Sci-ence and some of the Amplification Products available from Roche Applied Science contain this Water, PCR Grade. Storage and Stability Store a working solution for a short period of time
(e.g. 1 week) at 2 - 8 C Store stock solutions for a longer period of time
at 15 to 25 C Quality Control Test buffer: 10 mM Tris/HCl, pH 7.5 (37C), 10 mM MgCl 2 , 1 mM DTE Absence of endonucleases: 1 g DNA is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable degradation of the DNA. Absence of endonucleases: 1 g DNA, cleaved by Eco RI and Hind III (MWM III) is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade sh ows no detectable degradation of the DNA fragments. Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: 5 g of MS2 RNA are incubated with various amouts of Water, PCR Grade in 50 l test buffer at 37C for 1 h. Up to 20 l Water, PCR Grade shows no detectable RNAse activity. Amplification function test: Each lot of Water, PCR Grade is function tested in PCR. Routinely, PCR is performed using DNA for an 0.5 kb fragment. A clear defined band from 0.01 ng DNA should be clearly visible. Notice to purchase

This product is optimized for use in the Polymerase Chain Reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche). No license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.


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