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PCR Grade Water

Cat. No. 3 315 932, 25 ml (25 vials of 1 ml)
Cat. No. 3 315 959, 25 ml (1 vials of 25 ml)
Cat. No. 3 315 843, 100 ml (4 vials of 25 ml)) Description Specially purified, double distilled, deionized, auto-claved water Free of detectable endonucleases, ribonucleases and nicking activity Routinely function checked in PCR pH at 20C: 8.3 Application This product is especially tested and qualified for use in all PCR or RT-PCR reactions, in conjunction with conventional thermocyclers or with the LightCycler Instrument available from Roche Applied Science. All LightCycler Kits available from Roche Applied Sci-ence and some of the Amplification Products available from Roche Applied Science contain this Water, PCR Grade. Storage and Stability Store a working solution for a short period of time
(e.g. 1 week) at 2 - 8 C Store stock solutions for a longer period of time
at 15 to 25 C Quality Control Test buffer: 10 mM Tris/HCl, pH 7.5 (37C), 10 mM MgCl 2 , 1 mM DTE Absence of endonucleases: 1 g DNA is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade shows no detectable degradation of the DNA. Absence of endonucleases: 1 g DNA, cleaved by Eco RI and Hind III (MWM III) is incubated with various amounts of Water, PCR Grade in 50 l test buffer at 65C for 16 h. Up to 20 l Water, PCR grade sh
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