Navigation Links
Optimized Imaging of Protein Gels Stained with Coomassie,,,Brilliant Blue Dye

New optical filter for Eagle Eye II still video system

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

Michelle Cayouette Jane Moores
Stratagene Cloning Systems, Inc.

Stratagene currently provides an optical bandpass filter with all Eagle Eye II still video systems that is optimized for visualizing ethidium bromide fluorescence. However, a single filter specifically designed for ethidium bromide may be inadequate for use with other fluorophores or stains. In this report, we describe an optical filter designed for use with protein gels stained with Coomassie brilliant blue dye. The Coomassie blue bandpass filter enhances contrast and sensitivity for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was further increased when Coomassie blue-stained gels were imaged using a combination of the Coomassie blue-optimized filter and overhead illumination with the gel resting on top of a piece of white acrylonitrile butadiene styrene (ABS) plastic. The addition of the Coomassie blue bandpass filter expands the line of optical filters to the Eagle Eye II system optimized for specific applications.

Optical filters are placed in front of a camera lens to isolate or enhance the contrast between specific wavelengths of light. Stratagenes line of optical filters for the Eagle Eye II still video system currently consists of two filters. The bandpass filter, provided as part of the Eagle Eye II still video system, is designed to maximally overlap the emission spectrum of ethidium bromide, a fluorophore that emits orange light upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows orange fluorescent light to pass to the charge-coupled camera (CCD) camera, excluding all non-orange wavelengths of the spectrum. The other
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
2. Optimized Dengue RT-PCR
3. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
4. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
5. Imaging Chemiluminescent Western Blots
6. Imaging and Analysis of DIGE Product Information Sheet, Rev B
7. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
8. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
9. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
10. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
11. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:9/1/2015)... Sept. 1, 2015 The American Brain Tumor ... for its Discovery Research Grant program. LOIs are due ... noon CDT. Discovery Grants are one-year, $50,000 ... potential to change current diagnostic or treatment paradigms for ... encourage novel research at its earliest stages, in the ...
(Date:9/1/2015)... YORK , Sept. 1, 2015 /PRNewswire-USNewswire/ ... of cancer death in American men. One ... cancer and 233,000 new cases are diagnosed annually. ... experts are using a powerful new technique, ... small and large cancer lesions, treating only the ...
(Date:9/1/2015)... ... 2015 , ... In her thought provoking debut novel ‘ Ebola Vaccine Wars ... faced with an Ebola pandemic that is on the cusp of wiping out humanity. ... world of counterterrorism, and who continues to appear in this series of books by ...
(Date:9/1/2015)... ... September 01, 2015 , ... ... , Equine cervical facet joint problems can be caused by injuries and ... cause impingement of nerve roots or of the spinal cord itself. This can ...
Breaking Biology Technology:American Brain Tumor Association Seeks Innovative Concepts For Discovery Research Grants 2September Is Prostate Cancer Awareness Month 2September Is Prostate Cancer Awareness Month 3September Is Prostate Cancer Awareness Month 4Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 2Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 3VetStem Biopharma Announces New Clinical Research Program for Equine Cervical Facet Joint Disease 2VetStem Biopharma Announces New Clinical Research Program for Equine Cervical Facet Joint Disease 3
... Incorporated,(Nasdaq: SPEX ) reported a net loss for the three months ended March 31,2008 ... on the company,s on-going Phase 3 clinical trial,in the use of Naturlose for the treatment ... million and reflected the,start-up costs for the trial., , ... ...
... their 2008 Annual,Meeting, shareholders of Spherix Incorporated (Nasdaq: SPEX ) ... Douglas T. Brown, Senior VP and Manager of Corporate Banking Government ... Cox, Jr., Principal, Asset Protection Company -- Claire L. Kruger, ... Spherix -- Gilbert V. Levin, Ph.D., Director of Science and ...
... Executive Chairman Set Sights on Expanding,Therapeutic Horizons of ... BOSTON, SAN DIEGO, SAN FRANCISCO and SEATTLE, May ... joined Fate Therapeutics as,president and CEO and executive ... internationally recognized stem cell scientists,clinicians and investors dedicated ...
Cached Biology Technology:Spherix Reports 1st Quarter Earnings 2Spherix Holds Annual Shareholders Meeting 2Fate Therapeutics Taps Successful Biotech CEOs Paul Grayson and John Mendlein for Leadership Team 2Fate Therapeutics Taps Successful Biotech CEOs Paul Grayson and John Mendlein for Leadership Team 3Fate Therapeutics Taps Successful Biotech CEOs Paul Grayson and John Mendlein for Leadership Team 4Fate Therapeutics Taps Successful Biotech CEOs Paul Grayson and John Mendlein for Leadership Team 5Fate Therapeutics Taps Successful Biotech CEOs Paul Grayson and John Mendlein for Leadership Team 6
(Date:9/1/2015)... MOUNTAIN VIEW, Calif. , Sept. 1, 2015 ... interaction with travelers during airport passenger screening. This ... which the passenger screening process will become unintrusive. ... and pre-clearance based on voluntary background investigations will ... analysis from Frost & Sullivan, Global Airport ...
(Date:8/25/2015)... BELLEVUE, Wash. , Aug. 25, 2015 ... systems, announced today it will unveil its "Guardian S" ... at the National Tactical Officers Association Conference on August ... Guardian S is the first-ever commercially available energetically autonomous ... research and in-field trials and is protected by more ...
(Date:8/20/2015)... CT ., Aug. 20, 2015 NXT-ID, ... a biometric authentication company focused on the growing mobile ... announces it has joined The Smart Card Alliance and ... Smart Card Alliance and the Smart Card Alliance Latin ... visibility to industry peers and thought leaders promoting adoption ...
Breaking Biology News(10 mins):The Future of Airport Security: Passenger Screening Transitioning Into an Invisible Process 2The Future of Airport Security: Passenger Screening Transitioning Into an Invisible Process 3The Future of Airport Security: Passenger Screening Transitioning Into an Invisible Process 4Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4
... biosensor microchip that could significantly speed up the ... highly sensitive "nanosensors," analyze how proteins bind to ... effectiveness and possible side effects of a potential ... nanosensors can simultaneously and continuously monitor thousands of ...
... oil spill caused social disruption and psychological stress among ... the Exxon Valdez spill and the impacts are likely ... "Just ask the residents of Cordova today whether they ... Ritchie, assistant director for research of the University of ...
... study by researchers at the Virginia Institute of Marine ... does kill large numbers of copepodstiny crustaceans that are ... studyby VIMS graduate student Samantha Bickel, VIMS professor Kam ... the March issue of the Journal of Experimental ...
Cached Biology News:New biosensor microchip could speed up drug development, Stanford researchers say 2New biosensor microchip could speed up drug development, Stanford researchers say 3Gulf oil spill similar to Exxon Valdez in initial social and mental impacts, study finds 2VIMS study: Propeller turbulence may affect marine food webs 2VIMS study: Propeller turbulence may affect marine food webs 3
... This CLS number is ... to easily match Cornings product ... yet, please order under the ... contact customer service for assistance. ...
... Low Speed Shaker ,Product Information ... shaker is a high quality, general purpose shaker ... after the other models in the line, the ... easy-access control panel. Electronic controls are utilized for ...
... a chromogenic medium for the detection, ... (except S. thyphi) and total coliforms ... takes a maximum of 24 hours, ... sterilization during preparation and performs a ...
... ActiveSight, the leading provider of X-ray ... offers fragment-based screening services to its ... involves the binding of drug-like fragments ... protein drug targets, allowing scientists to ...
Biology Products: