Navigation Links
Optimized Imaging of Protein Gels Stained with Coomassie,,,Brilliant Blue Dye

New optical filter for Eagle Eye II still video system

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

Michelle Cayouette Jane Moores
Stratagene Cloning Systems, Inc.

Stratagene currently provides an optical bandpass filter with all Eagle Eye II still video systems that is optimized for visualizing ethidium bromide fluorescence. However, a single filter specifically designed for ethidium bromide may be inadequate for use with other fluorophores or stains. In this report, we describe an optical filter designed for use with protein gels stained with Coomassie brilliant blue dye. The Coomassie blue bandpass filter enhances contrast and sensitivity for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was further increased when Coomassie blue-stained gels were imaged using a combination of the Coomassie blue-optimized filter and overhead illumination with the gel resting on top of a piece of white acrylonitrile butadiene styrene (ABS) plastic. The addition of the Coomassie blue bandpass filter expands the line of optical filters to the Eagle Eye II system optimized for specific applications.

Optical filters are placed in front of a camera lens to isolate or enhance the contrast between specific wavelengths of light. Stratagenes line of optical filters for the Eagle Eye II still video system currently consists of two filters. The bandpass filter, provided as part of the Eagle Eye II still video system, is designed to maximally overlap the emission spectrum of ethidium bromide, a fluorophore that emits orange light upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows orange fluorescent light to pass to the charge-coupled camera (CCD) camera, excluding all non-orange wavelengths of the spectrum. The other
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
2. Optimized Dengue RT-PCR
3. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
4. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
5. Imaging Chemiluminescent Western Blots
6. Imaging and Analysis of DIGE Product Information Sheet, Rev B
7. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
8. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
9. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
10. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
11. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:10/20/2014)... 2014 Convey Computer , the ... from Iowa State University won first place in the ... Convey HC-2ex, the team’s solution achieved the highest overall ... the second place finisher. , Experts from all segments ... month long challenge, using a variety of design tools, ...
(Date:10/20/2014)...  GenVec, Inc. (NASDAQ: GNVC ) today announced ... its board of directors effective on October 24, 2014.   Dr. ... as its chairman from June 2006 to November 2013.  During ... Governance and Audit Committees of the board.  ... dedicated service to GenVec, and its stockholders," said Wayne ...
(Date:10/20/2014)... OR (PRWEB) October 20, 2014 Local ... participate in an investigational study of donor stem cells ... in stem cell therapy and has performed clinical stem ... this study is to determine if a single injection ... affected joints can help reduce pain and inflammation in ...
(Date:10/19/2014)... 19, 2014 The Asia-Pacific Bromine ... in Asia-Pacific with analysis and forecast of revenue. ... Pacific Bromine Market report, to get an idea ... a glimpse of the segmentation in the Asia-Pacific ... and figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , ...
Breaking Biology Technology:Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 2Team from Iowa State Wins 2014 MemoCODE Design Contest Using Convey HC-2ex 3Zola P. Horovitz To Retire From GenVec Board 2Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3
... Pharmaceutical Institute, the leading provider of specialized ... and a subsidiary of Campbell Alliance, today announced ... Excellence® Account Management Suite of e-courses.  The ... managed care account managers by addressing high-priority topics ...
... pairs the individual molecules that make up DNA ... medicine. Being able to isolate, study and sequence these ... therapies and treatments based on each patient,s individual genetic ... molecules like DNA base pairs, which are just two ...
... (OTC Bulletin Board: BIOR ), producer of ... disposal, announced today the Canadian Intellectual Property Office has ... Streamway System "for disposing of liquid surgical waste for ... the Company in April of 2011. Chad ...
Cached Biology Technology:Account Management Training Suite Delivers Two Key Performance Dimensions 2Particle trap paves way for personalized medicine 2BioDrain Medical, Inc. Granted Canadian Patent for its Streamway® Surgical Fluid Management System 2BioDrain Medical, Inc. Granted Canadian Patent for its Streamway® Surgical Fluid Management System 3
(Date:10/18/2014)... for evaluation of suspected genetic conditions, whole-exome sequencing ... including detection of a number of rare genetic ... to a study appearing in JAMA . ... the American Society of Human Genetics annual meeting. ... regions of thousands of genes simultaneously using next-generation ...
(Date:10/17/2014)... Researchers from the University of Copenhagen have shown for ... the lungs of Cystic fibrosis patients, giving them the ... growth in chronic infections. , The study also discovered ... fibrosis (CF) patients was halted or slowed down by ... consumed all the oxygen and helped "suffocate" the bacteria, ...
(Date:10/16/2014)... a new synthesis of recent research findings to inform ... the two states. , The Ecology and Management ... A Synthesis of the Relevant Biophysical Science and Implications ... by the U.S. Forest Service,s Pacific Northwest Research Station, ... for a synthesis of the large body of scientific ...
Breaking Biology News(10 mins):Whole-exome sequencing shows potential as diagnostic tool 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2New report synthesizes best available science on management of moist mixed-conifer forests 2
... gene responsible for the single most common cause of ... for the first time, a scientist told the annual ... Nice, France. Ms Melissa Thys, from the Department of ... finding may be a step towards new treatments for ...
... familiar with potato late blight, the plant disease responsible ... has the potential to be as devastating to economies ... The American Phytopathological Society (APS). , According to ... Plant Sciences Department at the University of Wyoming, Laramie, ...
... could not maintain their existence without thousands of proteins ... can cause disease, the study of protein structure and ... treatments for numerous disorders. For example, the discovery of ... development of a treatment based on insulin injections. Yet, ...
Cached Biology News:Gene responsible for common hearing loss identified for first time 2Potato wart eyed as risk to potato production 2Weizmann Institute scientists develop a general 'control switch' for protein activity 2Weizmann Institute scientists develop a general 'control switch' for protein activity 3
Sf21 Insect Cells (Live) in Max-XP Medium...
... The BioGenex Super ... System is a novel ... proprietary non-biotin polymeric technology. ... high signal to noise ...
Artisan™ Wash Solution (x 50) Additional Information: EU regulatory status: CE US regulatory status: IVD ...
MiniCycler thermal cycler with Hot Bonnet heated lid, holds 25x0.2ml tubes...
Biology Products: