Navigation Links
Optimized Imaging of Protein Gels Stained with Coomassie,,,Brilliant Blue Dye

New optical filter for Eagle Eye II still video system

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

Michelle Cayouette Jane Moores
Stratagene Cloning Systems, Inc.

Stratagene currently provides an optical bandpass filter with all Eagle Eye II still video systems that is optimized for visualizing ethidium bromide fluorescence. However, a single filter specifically designed for ethidium bromide may be inadequate for use with other fluorophores or stains. In this report, we describe an optical filter designed for use with protein gels stained with Coomassie brilliant blue dye. The Coomassie blue bandpass filter enhances contrast and sensitivity for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was further increased when Coomassie blue-stained gels were imaged using a combination of the Coomassie blue-optimized filter and overhead illumination with the gel resting on top of a piece of white acrylonitrile butadiene styrene (ABS) plastic. The addition of the Coomassie blue bandpass filter expands the line of optical filters to the Eagle Eye II system optimized for specific applications.

Optical filters are placed in front of a camera lens to isolate or enhance the contrast between specific wavelengths of light. Stratagenes line of optical filters for the Eagle Eye II still video system currently consists of two filters. The bandpass filter, provided as part of the Eagle Eye II still video system, is designed to maximally overlap the emission spectrum of ethidium bromide, a fluorophore that emits orange light upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows orange fluorescent light to pass to the charge-coupled camera (CCD) camera, excluding all non-orange wavelengths of the spectrum. The other
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
2. Optimized Dengue RT-PCR
3. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
4. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
5. Imaging Chemiluminescent Western Blots
6. Imaging and Analysis of DIGE Product Information Sheet, Rev B
7. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
8. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
9. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
10. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
11. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:8/26/2014)... what they believe is the thinnest-possible semiconductor, a ... only three atoms thick. , The University of ... single-layer semiconductor materials can be connected in an ... result could be the basis for next-generation flexible ... and solar technologies. , "Heterojunctions are fundamental elements ...
(Date:8/26/2014)... , Aug. 26, 2014 An analysis by ... of all Mers CoV virus strains has ... back to 2012.  This conservation and sharing of specific ... Synthetic Mers CoV Blocker-Vaccine™ candidate, as it did for the ... both of which have been found to be effective ...
(Date:8/26/2014)... added to the growing case for graphene being ... thing in the high-tech world by the two-dimensional ... of researchers led by a scientist with the ... Laboratory (Berkeley Lab) has reported the first experimental ... materials. The recorded charge transfer time clocked in ...
(Date:8/26/2014)... Ga. , Aug. 26, 2014  ViveBio, ... biological sample collection, stabilization and transportation, announced today ... Laboratory Procurement Services, LLC (LPS) of ... of products in the United States ... new class of ambient temperature dried biological specimen ...
Breaking Biology Technology:Scientists craft atomically seamless, thinnest-possible semiconductor junctions 2Competition for graphene 2ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2
... Transgenomic, Inc. (OTC/BB: TBIO) today reported financial results for ... business update. Second Quarter Financial ResultsNet sales ... increase of 50 percent compared with $5.1 million for the ... 59 percent of net sales, compared with gross profit of ...
... BETHESDA, Md., Aug. 11, 2011 The American College ... new ACMG Online Live Learning Center , which ... health care professionals. This valuable, on-demand resource brings the ... live learning offerings such as the Genetics Review Course ...
... GNVC ) today announced that its Board of ... through the adoption of a new Rights Agreement. The new ... 2011, which is the date that GenVec,s existing Rights Agreement ... in connection with the expiration of the existing Rights Agreement ...
Cached Biology Technology:Transgenomic Reports Second Quarter Financial Results 2Transgenomic Reports Second Quarter Financial Results 3Transgenomic Reports Second Quarter Financial Results 4Transgenomic Reports Second Quarter Financial Results 5Transgenomic Reports Second Quarter Financial Results 6Transgenomic Reports Second Quarter Financial Results 7Transgenomic Reports Second Quarter Financial Results 8Transgenomic Reports Second Quarter Financial Results 9ACMG Announces New Online Live Learning Center: Will Bring Genetics and Genomics Education to Varied Health Professionals 2GenVec Renews Stockholder Rights Plan 2GenVec Renews Stockholder Rights Plan 3
(Date:8/27/2014)... basic research, a growing focus on translating discoveries ... have earned the Herbert Irving Comprehensive Cancer Center ... NewYork-Presbyterian Hospital an $18 million, five-year Cancer Center ... The grant renews the center,s status as one ... New York City and one of only three ...
(Date:8/27/2014)... trans-fats have largely succeeded in sidelining the use ... for its potential contributions to Americans, health conditions. ... the best way to assuage the nation,s insatiable ... Chemical & Engineering News (C&EN), the weekly ... In the article, Stephen Ritter, a senior correspondent ...
(Date:8/27/2014)... thought to be associated with outcomes after the ... of the patient, mechanism of injury, nerve injured, ... operation technique, and repair materials. However, despite numerous ... nerve injuries, there is no agreement regarding the ... dose-effect relationship of the predictors has not been ...
Breaking Biology News(10 mins):Herbert Irving Comprehensive Cancer Center awarded $18 million grant 2Herbert Irving Comprehensive Cancer Center awarded $18 million grant 3Herbert Irving Comprehensive Cancer Center awarded $18 million grant 4
... sciences professor Harris Lewin is a recipient of the ... with James R. Cook, of Washington State University. ... recipients in agriculture, chemistry, physics, mathematics, medicine and the ... significant discoveries" in the field of animal agriculture. ...
... from the Walter and Eliza Hall Institute have begun ... a key gene required for the process. The ... blood stem cells, unique ability to self-renew could give ... tissue repair, transplantation and other therapeutic applications. Professor ...
... This release is available in Spanish . ... refrigerators that an estimated 20 percent are lost to spoilage, ... with USDA,s Agricultural Research Service (ARS) are working with colleagues ... The research also may lead to tomatoes that taste better ...
Cached Biology News:Illinois professor awarded 2011 Wolf Prize in Agriculture 2Erg gene key to blood stem cell 'self-renewal' 2Finding a way to extend tomato shelf-life 2
ANTI S. ENT TOX BC2D...
Porcine Serum US Origin 6 Months or Older...
... PlusOne Silver Staining Kit, Protein, ... Heukeshoven and Dernick (1), the kit ... gel electrophoresis systems.Silver Staining Kit, Protein ... of proteins in polyacrylamide gels in ...
All serum is collected from adult animals aged 6 months or older. Certified U.S. orgin. Pricing: $35/unit for 1 - 10 units...
Biology Products: