Navigation Links
Optimized Imaging of Protein Gels Stained with Coomassie,,,Brilliant Blue Dye

New optical filter for Eagle Eye II still video system

Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye

Michelle Cayouette Jane Moores
Stratagene Cloning Systems, Inc.

Stratagene currently provides an optical bandpass filter with all Eagle Eye II still video systems that is optimized for visualizing ethidium bromide fluorescence. However, a single filter specifically designed for ethidium bromide may be inadequate for use with other fluorophores or stains. In this report, we describe an optical filter designed for use with protein gels stained with Coomassie brilliant blue dye. The Coomassie blue bandpass filter enhances contrast and sensitivity for stained protein gels when compared to the ethidium bromide-optimized filter. Sensitivity was further increased when Coomassie blue-stained gels were imaged using a combination of the Coomassie blue-optimized filter and overhead illumination with the gel resting on top of a piece of white acrylonitrile butadiene styrene (ABS) plastic. The addition of the Coomassie blue bandpass filter expands the line of optical filters to the Eagle Eye II system optimized for specific applications.

Optical filters are placed in front of a camera lens to isolate or enhance the contrast between specific wavelengths of light. Stratagenes line of optical filters for the Eagle Eye II still video system currently consists of two filters. The bandpass filter, provided as part of the Eagle Eye II still video system, is designed to maximally overlap the emission spectrum of ethidium bromide, a fluorophore that emits orange light upon excitation with ultraviolet light. This ethidium bromide-optimized filter preferentially allows orange fluorescent light to pass to the charge-coupled camera (CCD) camera, excluding all non-orange wavelengths of the spectrum. The other
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
2. Optimized Dengue RT-PCR
3. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
4. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
5. Imaging Chemiluminescent Western Blots
6. Imaging and Analysis of DIGE Product Information Sheet, Rev B
7. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
8. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
9. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
10. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
11. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:7/7/2015)... 2015  SME,s Advanced Manufacturing Media is proud to ... issue, celebrating young men and women who have demonstrated ... issue of Manufacturing Engineering magazine ... their accomplishments. Among the standouts: Christopher Tyler , ... optimizing the topology of flapping wing vehicles; Kathryn ...
(Date:7/7/2015)... ... July 07, 2015 , ... uBiome, the leading microbial genomics company, ... microbiome sampling kits and full 16S laboratory analysis. The goal is to fast-track a ... also have access to their own dashboard, enabling them to view and compare the ...
(Date:7/7/2015)... ... , ... Since its inception in 2004 PhUSE has become ... conference for European Statistical Programmers, has evolved in the past eleven years to ... Data Managers and Biostatisticians all over the world, share, contribute and advance at ...
(Date:7/7/2015)... Pensacola, FL (PRWEB) , ... July 07, 2015 ... ... trials, ActiGraph’s CentrePoint Study Admin system was developed to solve many ... collection and analysis, particularly within studies that involve multiple clinical sites and/or remote ...
Breaking Biology Technology:SME's Advanced Manufacturing Media Recognizes "30 Under 30" Future Leaders of Manufacturing 2SME's Advanced Manufacturing Media Recognizes "30 Under 30" Future Leaders of Manufacturing 3SME's Advanced Manufacturing Media Recognizes "30 Under 30" Future Leaders of Manufacturing 4SME's Advanced Manufacturing Media Recognizes "30 Under 30" Future Leaders of Manufacturing 5uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 2uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 3PhUSE Delighted to Welcome Member Number 5.000 2ActiGraph Launches CentrePoint Cloud-based Study Management Software Platform 2ActiGraph Launches CentrePoint Cloud-based Study Management Software Platform 3
... Cardium Therapeutics (Amex:,CXM) announced today that Richard A. ... is an early pioneer of coronary stent,technology and is ... stent, the Palmaz-Schatz stent. Dr. Schatz is Research Director ... at,Scripps Clinic in La Jolla, California. He is also ...
... ... in Rockville, MD, ... NVAX ), the State of Maryland and Montgomery County ... the company,s headquarters in Rockville, Maryland. The 5,000,square-foot, $5 million ...
... "Anatomy of a Deal - BioMS Medical and Eli ... - BioMS Medical Corp (TSX: MS),a leading developer in ... Kevin Giese, President and CEO, BioMS Medical and Buu ... will take part in a speciality panel session at ...
Cached Biology Technology:Richard A. Schatz, M.D. Joins Cardium's Scientific Advisory Board 2Richard A. Schatz, M.D. Joins Cardium's Scientific Advisory Board 3Richard A. Schatz, M.D. Joins Cardium's Scientific Advisory Board 4Novavax Opens Its First U.S. Vaccine Plant 2Novavax Opens Its First U.S. Vaccine Plant 3Novavax Opens Its First U.S. Vaccine Plant 4BioMS Medical to Participate in Speciality Panel Session at BioFinance 2008 2BioMS Medical to Participate in Speciality Panel Session at BioFinance 2008 3
(Date:6/23/2015)... 23, 2015   Valencell, a leader ... of a recent study that illustrates its PerformTek ... during activity. In a study conducted at Valencell,s ... with the Apple Watch against a chest strap ... during activity. The study demonstrated that Valencell,s biometric ...
(Date:6/18/2015)... , June 18, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market announces that ... featured segment on "Money on the Mark", scheduled to air ... June 20 th . The ... be from 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO ...
(Date:6/17/2015)... 2015  Synaptics Inc. (NASDAQ: SYNA ), ... that Xiaomi, one of the world,s largest mobile ... ® family of capacitive touchscreen solutions and ... for its latest smartphones, the Xiaomi Mi Note ... in-cell display solutions and DDICs in discrete display ...
Breaking Biology News(10 mins):Valencell Delivers Breakthrough Heart Rate Accuracy on the Wrist 2Valencell Delivers Breakthrough Heart Rate Accuracy on the Wrist 3NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3
... will use a $120,000 grant from the Foundation for AIDS ... cells it attacks have evolved together over time. The goal ... When HIV infiltrates cells, the virus hijacks its host,s ... has been recently found that the retrovirus hijacks about 1,000 ...
... by researchers at the Montreal Neurological Institute (MNI), ... provides vital insights into the neurological basis of ... some instances develop various addictions when undergoing medical ... 25) issue of the scientific journal Neuron ...
... new statistical model could be used to predict an ... the Children,s Hospital Informatics Program (CHIP). Using genetic information ... predictive model could estimate an individual,s overall risk of ... -- with 86 percent accuracy. The findings are reported ...
Cached Biology News:Evolutionary biologist will study HIV with grant from AIDS research foundation 2Addiction: Insights from Parkinson's disease 2Predicting risk of stroke from one's genetic blueprint 2
... any scanner into a gel densitometer. Although ... the basic operation simply consists of converting ... into pixel density / intensity values through ... Turn your scanner into a high speed ...
... Signets FAST RED Chromogen System consists of ... RED reacts in the presence of alkaline ... background noise. The FAST RED System ... ,The System Contains: , , Vial A: ...
Mouse polyclonal antibody to QDPR - quinoid dihydropteridine reductase...
... silver stain kits are optimized for ... Bio-Rad Dodeca stainers. This silver stain ... proteins in polyacrylamide gels and are ... sensitizer concentrate, silver reagent concentrate, background ...
Biology Products: