Navigation Links
Nocardia corallina

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.526 01/2002 Microorganism Nocardia corallina Cell type Bacteria, gram positive Molecules injected Plasmid DNA (in TE buffer) Growth medium N2 medium (20 g/l glucose, 2 g/l yeast extract, 4 g/l beef extract, 6 g/l tryptone, 2 g/l NaCl, 10 g/l glycine) Washing solution Distilled water Electroporation solution 0.3 M sucrose, 15% glycerol Outgrowth medium N2 medium (without antibiotics) Cuvette 2 mm gap width Reference Yao W., et al 1994 Current Microbiology 29 223-227 Making electrocompetent cells:

1. Inoculate a fresh overnight culture of bacteria into N2 medium. Grow cells at 28 C to an O.D.600 of 0.7. Harvest by centrifugation.. 2. Wash twice with _ volume of distilled water. 3. Resuspend in a final volume of 1/20 of the original volume of electroporation solution.

Electroporation of cel ls:

  1. Add 2 l (0.1 g) plasmid DNA to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
  4. Add 1 ml N2 medium and incubate for 2 hours at 30 C.
  5. Plate cells on selective YEME medium with a 3 ml YEME soft agar (0.4%) overlayment.
Expected Results: Transformation efficiency up to 2.8 x 103 transformants/g of DNA.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Nocardia asteroides
2. Nocardia corallina
3. Nocardia asteroides
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/16/2017)... 16, 2017  Eurofins Genomics today announced the expansion ... customers to receive their primers in a shorter turnaround ... quality found with other providers. Express oligos are available ... at no additional fee. Researchers use ... including DNA sequencing, genotyping, site-directed mutagenesis, and cloning. Often, ...
(Date:1/13/2017)... , Jan 13, 2017 Research and Markets has ... to their offering. ... global biopolymers market to grow at a CAGR of 16.83% during the ... the growth prospects of the global biopolymers market for 2017-2021. To calculate ... of sales of biopolymer products. The report also includes a a discussion ...
(Date:1/12/2017)... ... January 12, 2017 , ... ... INTERFUSE® IS A SUCCESS , VTI, Vertebral Technologies, Inc., announces the successful ... laterally expandable device. Since September 2016, VTI (Vertebral Technologies, Inc.) has partnered ...
(Date:1/12/2017)... , ... January 12, 2017 , ... ... targeted treatments, 26-year-old Lisa Rosendahl’s doctors gave her only a few months to ... new drug combination that has stabilized Rosendahl’s disease and increased both the quantity ...
Breaking Biology Technology:
(Date:12/22/2016)... 2016  As part of its longstanding mission to improve ... company, recently released its latest children,s book, titled ... on the topics of inheritance and variation of traits that ... in elementary school classrooms in the US. ... Ariana Killoran , whose previous book with 23andMe, ...
(Date:12/20/2016)... 20, 2016   Valencell , the leading ... STMicroelectronics (NYSE: STM), a global semiconductor leader ... announced today the launch of a new, highly ... that includes ST,s compact SensorTile turnkey ... biometric sensor system. Together, SensorTile and Benchmark deliver ...
(Date:12/16/2016)... Research and Markets has announced the addition of the "Biometric ... to their offering. ... The biometric vehicle access system market, in terms of value, ... to 2021. The market is estimated to be USD 442.7 Million ... The growth of the biometric vehicle access system market is fueled ...
Breaking Biology News(10 mins):